Comparative study on the hemocytes of subtropical oysters Saccostrea kegaki (Torigoe &amp; Inaba, 1981), Ostrea circumpicta (Pilsbry, 1904), and Hyotissa hyotis (Linnaeus, 1758) in Jeju Island, Korea: Morphology and functional aspects

Hong, Hye Kyoung; Kang, Hyun-Sil; Le, Thanh Cuong; Choi, Kwang-Sik

doi:10.1016/j.fsi.2013.09.022

Cited by 27 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some bivalves also have small hyalinocytes with a large nucleus to cytoplasm ratio sometimes referred to as blast-like cells or hemoblasts. These cells have already been observed in many bivalve species including Tapes philippinarum (Cima et al, 2000), Saccostrea glomerata (Aladaileh et al, 2007), Crassostrea ariakensis (Donaghy et al, 2009), Saccostrea kegaki, Ostrea circumpicta, Hyotissa hyotis (Hong et al, 2013). Their presence in a variety of bivalve species, low immune function capacity (Donaghy et al, 2009), positive reading to the mammalian stem cell molecular marker anti-CD34 (in the case of T. philippinarum) (Cima et al, 2000), and the ability to divide (as found in T. philippinarum) (Matozzo et al, 2008) all provide evidence to suggest blast-like cells may actually stem cells that mature into the other cell types.…”

Section: Introductionmentioning

confidence: 73%

“…The function of this cell type in bivalves is unknown although its morphology may be the result of a granulocyte going through degranulation. This cell type has been reported in the mussel Anodonta cygnea (Salimi et al, 2009) oysters S. kegaki, O. circumpicta, H. hyotis, C. gigas (Chang et al, 2005Hong et al, 2013), the cockle Cerastoderma edule (Russell-Pinto et al, 1994), and the clam Scapharca inaequivalvis (Holden et al, 1994). The serous cell is rounded and has a light brown to almost black coloration to it when viewed unstained (Cheng, 1981;Haszprunar, 1996).…”

Section: Introductionmentioning

confidence: 74%

“…The observations made on the hemocytes of the freshwater gastropod Planorbarius corneus support this theory (Franceschi et al, 1991). We chose to label these cells with a more general term (small hyalinocytes) since functional studies will be needed to determine whether they have the relatively low immune function capability that is characteristic of blast-like cells (Hong et al, 2013;Kuchel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, the number of bivalve species covered in the hemocyte literature has continued to increase over the years. Bivalve species recently added to this literature base include Callista chione (Matozzo and Bailo, 2015), Bathymodiolus japonicas, B. platifrons, B. septemdierum (Tame et al, 2015), S. kegaki, O. circumpicta, H. hyotis (Hong et al, 2013), and A. cygnea (Salimi et al, 2009). The current study contributes to this effort by classifying the hemocytes of E. directus, and providing a detailed morphology of them using both transmission electron microscopy (TEM) and light microscopy.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Preziosi

Bowden

2016

Micron

View full text Add to dashboard Cite

The Atlantic jackknife clam, Ensis directus, is currently being researched as a potential species for aquaculture operations in Maine. The goal of this study was to describe the hemocytes of this species for the first time and provide a morphological classification scheme. We viewed hemocytes under light microscopy (using Hemacolor, neutral red, and Pappenheim's stains) as well as transmission electron microscopy (TEM). The 2 main types of hemocytes found were granulocytes and hyalinocytes (agranular cells). The granulocytes were subdivided into large and small granulocytes while the hyalinocytes were subdivided into large and small hyalinocytes. The large hemocytes had both a larger diameter and smaller nucleus to cell diameter ratio than their smaller counterparts. A rare cell type, the vesicular cell, was also observed and it possessed many vesicles but few or no granules. Using TEM, granulocytes were found to contain both electron-lucent and electron-dense granules of various sizes. These numerous granules were the only structures that took up the neutral red stain. Hyalinocytes had few of these granules relative to granulocytes. Large hyalinocytes had both various organelles and large vesicles in their abundant cytoplasm while small hyalinocytes had little room for organelles in their scant cytoplasm. Total hemocyte counts averaged 1.96×10(6) cells mL(-1) while differential hemocyte counts averaged 11% for small hyalinocytes, 12% for large hyalinocytes, 59% for small granulocytes, and 18% for large granulocytes. The results of this study provide a starting point for future studies on E. directus immune function.

show abstract

Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Preziosi

Bowden

2016

Micron

View full text Add to dashboard Cite

show abstract

“…The light microscopic analysis revealed that M. virgata had three distinct types of hemocytes in the hemolymph, as in other marine bivalves [Mytilus coruscus (Yang et al, 2015), Crassostrea gigas (Donaghy et al, 2010), C. ariakensis (Donaghy et al, 2009b), C. nippona (Hong et al, 2014), Saccostrea kegaki (Hong et al, 2013), S. glomerata (Aladaileh et al, 2007), Ostrea circumpicta (Hong et al, 2013), Hyotissa hyotis (Hong et al, 2013), Anodonta cygnea (Jamili et al, 2009)], including granulocytes, hyalinocytes, and blast-like cells (Figure 3). The granulocytes contained numerous granules in the cytoplasm, and several long pseudopodia were attached to the surface.…”

Section: Hemocyte Typesmentioning

confidence: 81%

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hong

Kim

et al. 2021

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

In the rocky intertidal environment, the frequency and duration of heatwaves have increased over the last decade, possibly due to global climate change. Heatwaves often result in lethal or sub-lethal disturbances in benthic animals by changing their metabolic activities. In this study, we investigated the impacts of extreme heatwave stress on the hemocyte functions of Mytilisepta virgata and subsequent mortality to gain a better understanding of the potential causes and consequences of mass mortality events in this mussel during summer. We discriminated three types of hemocytes in the hemolymph, granulocytes, hyalinocytes, and blast-like cells, using flow cytometry and revealed that granulocytes were the major hemocyte involved in cellular defensive activities, such as phagocytosis and reactive oxygen species (ROS) production. For the experiment, mussels were exposed to a 40°C air temperature for 12 h per day over 5 days under laboratory conditions as a simulated semi-diurnal tidal cycle. Mortality began to occur within 3 days after beginning the experiment, and all mussels had died by the end of the experiment. Flow cytometry indicated that the mussels exposed to high air temperatures produced significantly more ROS than did the control mussels within 2 days after the onset of the experiment, which may have caused oxidative stress. Such high levels of ROS in the hemolymph increased DNA damage in hemocytes after 3 days of exposure and decreased the phagocytosis of hemocytes 4 days after the experiment began. The observed mortality and decline in immune capacity suggested that an extreme heat event occurring in the rocky intertidal ecosystem during summer could exert sublethal to lethal impacts on macrobenthic animals.

show abstract

Hematology of Invertebrates

Arnold¹

2022

Schalm's Veterinary Hematology

View full text Add to dashboard Cite

Comparative study on the hemocytes of subtropical oysters Saccostrea kegaki (Torigoe & Inaba, 1981), Ostrea circumpicta (Pilsbry, 1904), and Hyotissa hyotis (Linnaeus, 1758) in Jeju Island, Korea: Morphology and functional aspects

Cited by 27 publications

References 35 publications

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hematology of Invertebrates

Contact Info

Product

Resources

About