Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

Abstract

Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach. In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0-8 h, 400 rpm during 8-12 h, and 600 rpm during 12-20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0-8 h and at 5% during 8-20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model. Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA production was achieved.

Biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus using oxygen vector and optimum impeller tip speed

“…However, a 30% increase of HA biosynthesis was obtained by adding 5% (v/v) n-dodecane while using S. zooepidemicus WSH-24 (5). Liu et al (22) used 3% (v/v) perfluorodecalin as the oxygen vector in their culture to enhance the HA concentration from 5.50 g HA/l to 6.60 g HA/l. The present work using n-hexadecane as an oxygen vector demonstrated an enhanced HA concentration together with its molecular weight.…”

“…Rols et al (14) have elucidated the mechanism of enhanced oxygen transfer using oxygen vectors. About 30% (w/v) increase in HA biosynthesis was obtained by adding 5% (v/v) n-dodecane (5) and 3% (v/v) perfluorodecalin (22).…”

Hyaluronic acid production by Streptococcus zooepidemicus in marine by-products media from mussel processing wastewaters and tuna peptone viscera

“…Although several strategies have been reported for increasing microbial HA, including pH-gradient stress [ 8 ], continuous culture [ 9 ], lysozyme or hyaluronidase addition [ 10 , 11 ], agitation and aeration conditions [ 12 - 14 ], medium optimisation [ 15 ], the type of bioreactor [ 16 ], effect of aminoacids and mineral salts [ 17 , 18 ] and fed-batch operation [ 19 ], there is almost no studies of new sources of sugars and proteins from organic waste materials in order to reduce both production costs and pollution problems. More than 80% of these costs are due to these nutrients (sugars and proteins) and commercial formulations are not an economical resource for industrial production of HA.…”

Chondroitin Sulfate, Hyaluronic Acid and Chitin/Chitosan Production Using Marine Waste Sources: Characteristics, Applications and Eco-Friendly Processes: A Review

“…Several culture variables have been studied and optimized such as lysozyme or hyaluronidase addition [91,92], agitation and aeration conditions [93,94,95], the type of bioreactor [96], effect of pH-gradient stress [97], continuous culture [98], medium optimization [99] and fed-batch operation [100]. Since no marine microorganisms have been discovered for HA production, the only marine approach for this bioproduction is derived from the substitution of commercial broths by alternative nutrients generated in the marine foodstuff manufacturing.…”