In this study, single-layer and three-layer nickel-based coatings were fabricated on 316L SS by laser additive manufacturing. The phase characterization, microstructure observation, and microhardness analysis of the coatings were carried out by X-ray diffraction (XRD), scanning electron microscope (SEM), and microhardness tester. And the wear resistance of the coatings was analyzed through dry sliding friction and wear test. The results show that the cross-section microstructure of the three-layer nickel-based coating is different from that of the single-layer one under the influence of heat accumulation; the dendrite structure in the central region of the former is equiaxial dendrite, while that of the latter still remains large columnar dendrites. The existence of solid solution phase γ-(Fe, Ni) and hard phases of Ni17Si3, Cr5B3, Ni3B in the coating significantly improve the wear resistance of the coating, and the microhardness is nearly 2.5 times higher than that of the substrate. However, the average microhardness of multilayer cladding coating is about 48 HV0.2 higher than that of the single-layer cladding coating. In addition, the fine surface structure of the three-layer nickel-based coating improves the wear resistance of the coating, making this coating with the best wear resistance.