Comparative study on the mechanical property of silk thread from cocoons of Bombyx mori L.

Iizuka, Eisaku; Hachimori, Akira; K, Abe; Sunohara, M; Hiraide, Y; Ueyama, Akihiko; Kamo, Kazuhiko; Fujiwara, Takayuki; F, Nakamura; Uno, Tomohide

doi:10.3233/bir-1983-20504

Cited by 8 publications

(3 citation statements)

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“…hesperus were used to obtain various composite silkworm/spider silk fibres using B . mori as a platform, which has a similar mechanism for the spinning process to that of silk formation in the spider 40 . The amino-acid composition is highly similar between the silkworm and spider silk proteins, which consist of glycine-rich and alanine-rich proteins 10 , 41 .…”

Section: Discussionmentioning

confidence: 99%

Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin

You

et al. 2018

Sci Rep

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Spider dragline silk is a remarkable material that shows excellent mechanical properties, diverse applications, biocompatibility and biodegradability. Transgenic silkworm technology was used to obtain four types of chimeric silkworm/spider (termed composite) silk fibres, including different lengths of recombinant Major ampullate Spidroin1 (re-MaSp1) or recombinant Major ampullate Spidroin2 (re-MaSp2) from the black widow spider, Latrodectus hesperus. The results showed that the overall mechanical properties of composite silk fibres improved as the re-MaSp1 chain length increased, and there were significant linear relationships between the mechanical properties and the re-MaSp1 chain length (p < 0.01). Additionally, a stronger tensile strength was observed for the composite silk fibres that included re-MaSp1, which only contained one type of repetitive motif, (GA)n/An, to provide tensile strength, compared with the silk fibres that includedre-MaSp2, which has the same protein chain length as re-MaSp1 but contains multiple types of repetitive motifs, GPGXX and (GA)n/An. Therefore, the results indicated that the nature of various repetitive motifs in the primary structure played an important role in imparting excellent mechanical properties to the protein-based silk fibres. A silk protein with a single type of repetitive motif and sufficiently long chains was determined to be an additional indispensable factor. Thus, this study forms a foundation for designing and optimizing the structure of re-silk protein using a heterologous expression system.

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Section: Discussionmentioning

confidence: 99%

Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin

You

et al. 2018

Sci Rep

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“…Of all natural silk-producing animals, mulberry silkworms (Bombyx mori) are of the most economic importance, because it is possible to rear them in captivity. After the eggs laid by adult moths hatch, the caterpillars are fed fresh mulberry leaves for a month until they are mature enough for metamorphosis into a moth, which requires the construction of a protective cocoon of silk [45][46][47][48]. Once the cocoon is complete, silk farmers kill the caterpillar via exposure to hot water/steam, and the silk can be harvested.…”

Section: Silkworm Silkmentioning

confidence: 99%

Polymeric materials based on silk proteins

Hardy

Römer

Scheibel

2008

Polymer

466

373

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“…The amorphous phase is composed of hydrogen-bonded polypeptide chain segments rich in glycine residues such as GGX motifs that adopt still illdefined, but more disordered, secondary structures such as 3 1 -helices, β-spirals, turns and/or random coils, 55,[68][69][70][71][72] and that surprisingly seem to be only very slightly oriented. 70,73 A similar structural pattern is also observed for B. mori silk, although the crystallinity [74][75][76][77][78][79] and the proportion of β-sheets 70,73 are higher.…”

Section: Relation Between Primary and Secondary Structuresmentioning

confidence: 61%

Spider silk as a blueprint for greener materials: a review

Lefèvre

Auger

2016

International Materials Reviews

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Spider silk exhibits remarkable properties, especially its well-known tensile performances. They rely on a complex nanostructured hierarchical organization that researches progressively elucidate. Spider silk encompasses a vast range of fibers that exhibit diverse and captivating physical and biological characteristics. The full understanding of the relation between structure and properties may lead in the future to the design of a variety of high-performance, tailored materials and devices. Reknown for being produced in mild and begnin conditions, this outstanding biological material constitutes one the more representative example of biomimetism. In addition, silk's structure is produced with limited means, i.e. low energy and relatively simple renewable constituents (silk proteins). Then, if successfully controlled and adequately transposed in biomaterials, some properties of natural silk could lead to innovative green materials that may contribute to reduce the ecological footprint of societies. In fact, striking recent advanced applications made with B. mori silk suggest that spider silk-based materials may lead to advanced resistant and functional materials, then becoming among the most promising subject of study in material science. However, several challenges have to be overcome, especially our ability to produce native-like silk, to control biomaterials' structure and properties, and to minimize their ecological footprint. This paper reviews the characteristics of spider silk that make it so attractive and that may (or may not) contribute to reduce ecological footprint of materials and the challenges in producing innovative spider silk-based materials. First, from a biomimetic perspective, the structure and models that explain the tensile resistance of natural silk are presented, followed by the state of knowledge regarding natural silk spinning process and synthetic production methods. Biocompatibility (biosafety and biofunctionality) as well as biodegradability issues are then addressed. Finally, examples of applications are reviewed. Features that may lead to the design of green materials are emphasized throughout.

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Comparative study on the mechanical property of silk thread from cocoons of Bombyx mori L.

Cited by 8 publications

References 0 publications

Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin

Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin

Polymeric materials based on silk proteins

Spider silk as a blueprint for greener materials: a review

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