Comparative study on the structural characterization and α-glucosidase inhibitory activity of polysaccharide fractions extracted from Sargassum fusiforme at different pH conditions

Zheng, Qianwen; Jia, Rui-Bo; Ou, Zhirong; Li, Zhao-Rong; Zhao, Ming; Luo, Da; Lin, Lianzhu

doi:10.1016/j.ijbiomac.2021.11.103

Cited by 42 publications

(21 citation statements)

References 44 publications

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“…As a common analytical tool in the primary structure identification of polysaccharides, FT‐IR can be used to analyze the glycosidic bond type, functional group type, and sugar ring configuration of polysaccharides (Zheng et al., 2021). As can be seen from Figure 3a, all the three fractions polysaccharides have similar general spectral characteristics except some variations in the characteristic bands.…”

Section: Resultsmentioning

confidence: 99%

“…This rusult suggested that VL8‐EPS50 and VL8‐EPS70 might have higher bioactivity. Zeta potential is a key indicator of the attraction and repulsion between macromolecules and can be used to evaluate the stability of a substance (Zheng et al., 2021). The higher the absolute value of zeta potential, the higher the stability of the solution system (X. Yang et al., 2022).…”

Section: Resultsmentioning

confidence: 99%

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Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Liu

Mao

Zhang

et al. 2022

Journal of Food Science

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In this study, gradient ethanol precipitation method was applied to obtain the extracellular polysaccharides of Lactobacillus paracasei VL8 (VL8‐EPS). The yields, physicochemical properties, and immunomodulatory effects of VL8‐EPS obtained by precipitation at different ethanol concentrations (30%, 50%, and 70%, v/v) were compared. The results showed that VL8‐EPSs were high molecular weight sulfated heteropolysaccharides, composed mainly of glucose and galactose, and the alteration of ethanol concentration had an effect on their chemical compositions, molecular weight distributions, monosaccharide composition, and surface structure, while the primary structure remained the same. Among the three polysaccharide fractions, VL8‐EPS50 displayed better immunomodulatory activities compared with VL8‐EPS30 and VL8‐EPS70. VL8‐EPS50 was found to exert immunomodulatory effects by enhancing the phagocytic activity of RAW264.7 cells and to promote their secretion of more nitric oxide; it also showed stronger thermal and solution stability. In summary, there was a correlation between the structural characteristics of polysaccharides and their immunomodulatory activity, and VL8‐EPS50 was preferentially used for in vivo immunomodulatory activity. Practical ApplicationThis study opens up the source of raw materials for functional foods, which can provide some theoretical basis for the research and development of extracellular polysaccharides of lactic acid bacteria and promote their application in the future development of food industry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Liu

Mao

Zhang

et al. 2022

Journal of Food Science

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“…A polysaccharide fraction, AXA-1, isolated from wheat bran showed a potential non-competitive mode of inhibitory effects against the α-glucosidase enzyme [ 123 ]. Zheng et al [ 125 ] investigated the α-glucosidase inhibitory activity of several polysaccharides extracted from Sargassum fusiforme at different pH conditions. According to the study, SEP-7-40, which has relatively high levels of xylose and galacturonic acid and low molecular weight, exhibits a considerable inhibitory effect (IC 50 = 0.304 mg/mL).…”

Section: Plant-derived Bioactive Compounds As Potential α-Glucosidase...mentioning

confidence: 99%

Recent Updates on Phytoconstituent Alpha-Glucosidase Inhibitors: An Approach towards the Treatment of Type Two Diabetes

Kashtoh

Baek

2022

Plants

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Diabetes is a common metabolic disorder marked by unusually high plasma glucose levels, which can lead to serious consequences such as retinopathy, diabetic neuropathy and cardiovascular disease. One of the most efficient ways to reduce postprandial hyperglycemia (PPHG) in diabetes mellitus, especially insulin-independent diabetes mellitus, is to lower the amount of glucose that is absorbed by inhibiting carbohydrate hydrolyzing enzymes in the digestive system, such as α-glucosidase and α-amylase. α-Glucosidase is a crucial enzyme that catalyzes the final stage of carbohydrate digestion. As a result, α-glucosidase inhibitors can slow D-glucose release from complex carbohydrates and delay glucose absorption, resulting in lower postprandial plasma glucose levels and control of PPHG. Many attempts have been made in recent years to uncover efficient α-glucosidase inhibitors from natural sources to build a physiologic functional diet or lead compound for diabetes treatment. Many phytoconstituent α-glucosidase inhibitors have been identified from plants, including alkaloids, flavonoids, anthocyanins, terpenoids, phenolic compounds, glycosides and others. The current review focuses on the most recent updates on different traditional/medicinal plant extracts and isolated compounds’ biological activity that can help in the development of potent therapeutic medications with greater efficacy and safety for the treatment of type 2 diabetes or to avoid PPHG. For this purpose, we provide a summary of the latest scientific literature findings on plant extracts as well as plant-derived bioactive compounds as potential α-glucosidase inhibitors with hypoglycemic effects. Moreover, the review elucidates structural insights of the key drug target, α-glucosidase enzymes, and its interaction with different inhibitors.

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“…The relationship between M w and the elution volume is built according to a previously reported study. 33 Preliminary calibration is conducted using dextrans with various molecular weights (5,12,50,150,210, and 410 kDa) as references. The M w of the sample is estimated by the calibration equation established above.…”

Section: Measurement Of Ultraviolet (Uv) Spectramentioning

confidence: 99%

“…MMF can ameliorate the symptoms of dyspepsia by regulating the homeostasis of the brain–gut–microbiota axis, treating gastrointestinal inflammation, and improving intestinal microflora disturbances. − Digestive enzymes and lactic acids in MMF are considered to be bioactive constituents of MMF with only a few studies focusing on the function of polysaccharides in MMF . In recent decades, natural polysaccharides in Chinese herbs have received considerable attention because of their promising health-promoting functions, such as anti-tumor, anti-inflammatory, antioxidant, anti-diabetic, radiation-protecting, antiviral, hypolipidemic, and immunomodulatory activities . Therefore, research examining the polysaccharides in MMF should facilitate the development and application of MMF.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products

et al. 2022

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Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata). The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage. Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated. Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains. Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher. These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity. This research provides a guide for the selective application of Massa Medicata Fermentata.

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Comparative study on the structural characterization and α-glucosidase inhibitory activity of polysaccharide fractions extracted from Sargassum fusiforme at different pH conditions

Cited by 42 publications

References 44 publications

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Recent Updates on Phytoconstituent Alpha-Glucosidase Inhibitors: An Approach towards the Treatment of Type Two Diabetes

Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products

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