Comparative study on the use of solid media: Löwenstein–Jensen and Ogawa in the determination of anti-tuberculosis drug susceptibility

Tanoue, Shota; Mitarai, Satoshi; Shishido, Harumi

doi:10.1054/tube.2002.0323

Cited by 15 publications

(12 citation statements)

References 7 publications

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“…However, we may indirectly and roughly make an estimate using the proportion of sputum positive smears (confidence on this data is high as microscopy diagnosis was externally audited and considered to be 100% accurate for three years in a row) and considering the time to culture positivity data. Literature data evaluating the quality of direct sputum smear examination estimate that an excellent quality of direct examination generates around 65% microscopically confirmed cases out of all bacteriologically confirmed cases (Tanoue et al, 2002). This figure is close to the 70.3% value for our study group; a major sensitivity loss would have significantly increased the proportion of microscopically confirmed cases.…”

Section: Discussionsupporting

confidence: 83%

Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note

Cernomaz¹,

Peter²,

Adina-Catinca³

et al. 2015

Afr. J. Microbiol. Res.

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Mycobacteria culture remains the cornerstone of tuberculosis diagnosis. Naturally contaminated samples need pre-inoculation processing but some economically challenged medical facilities may benefit from a simpler and cheaper sputum decontamination procedure. The aim of this study was to test a simple decontamination method lacking a centrifugation step to be used in conjunction with the culture on Löwenstein-Jensen medium. A total of 7446 sputum samples collected from 3229 patients were microscopically examined and then cultured on Löwestein-Jensen medium using a simplified Petroff method. All positive cultures were confirmed by direct microscopic examination and biochemical identification. Culture and microscopic status and time to positivity were recorded. Mean and median times to culture and contamination rate were similar as compared to classical Löwenstein-Jensen culture method. Overall results suggest that the described modified of Petroff method may be used with adequate results in resource poor settings as the method does not require an aerosol safe centrifuge and relies on cheap, stable and readily available reagents.

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Section: Discussionsupporting

confidence: 83%

Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note

Cernomaz¹,

Peter²,

Adina-Catinca³

et al. 2015

Afr. J. Microbiol. Res.

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“…It is possible that Ogawa media is less sensitive than standard Löwenstein-Jensen media. However, there are several studies that showed that the sensitivities of Ogawa and Lö wenstein-Jensen media are similar [26][27][28]. In addition, Ogawa media is suitable for use in a TB endemic area because it is inexpensive and easy to prepare.…”

Section: Discussionmentioning

confidence: 99%

Nested PCR in lung tissue for diagnosis of pulmonary tuberculosis

Kang¹,

Kwon²,

Yoon³

et al. 2009

Eur Respir J

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Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB.Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies, followed by nested TB PCR on formalin-fixed paraffin-embedded lung tissue specimens. The diagnostic yield and clinical impact of nested PCR were investigated.Of the 223 patients studied, 142 were diagnosed with TB. Microbiologically confirmed TB was identified in 71 patients. Compared to culture results, the sensitivity, specificity, positive predictive value and negative predictive value of nested PCR were 85%, 99%, 98% and 88%, respectively. Nested PCR was more sensitive than acid-fast bacilli smear of respiratory specimens and histopathological findings. The PCR results provided an early diagnosis and initiation of treatment for TB. However, negative PCR results did not lead to discontinuation of unnecessary TB treatment in patients on medication.In conclusion, nested PCR on lung tissue specimens is a useful diagnostic test for pulmonary TB in patients with an unusual presentation.

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“…The in vitro antitubercular activity was screened against M. tuberculosis using egg base Lo¨wenstein-Jensen and Ogawa media (Tanoue et al, 2002), by measuring the growth of M. tuberculosis (H 37 RV) after 3 and 6 weeks. Cultures were incubated at 37°C in ambient air for up to 6 weeks.…”

Section: In Vitro Antitubercular Activitymentioning

confidence: 99%

Potent in vitro and in vivo antitubercular activity of certain newly synthesized indophenazine 1,3,5-trisubstituted pyrazoline derivatives bearing benzofuran

Manna

Agrawal

2010

Med Chem Res

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Fourteen newly synthesized derivatives of indophenazine 1,3,5-trisubstituted pyrazoline bearing benzofuran were prepared from benzofuran chalcones with indophenazine hydrazide through cycloaddition reaction. All the compounds were screened for their in vitro and in vivo antitubercular activity against drug resistant and multidrug-resistant Mycobacterium tuberculosis H 37 RV. The MIC 50 and MIC 90 were estimated and compared with rifampicin and gatifloxacin standard drugs. Nitro group containing at ortho 5j, meta 5e, furan ring containing 5m and ortho 5i, para 5h chloro containing compounds were exhibited significant in vitro, in vivo antitubercular activity against standard drugs.Keywords In vitro and in vivo antitubercular activity Á Multidrug-resistant M. tuberculosis Á Indophenazine pyrazoline Á Benzofuran

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Comparative study on the use of solid media: Löwenstein–Jensen and Ogawa in the determination of anti-tuberculosis drug susceptibility

Cited by 15 publications

References 7 publications

Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note

Alternative technique for culturing sputum for mycobacteria isolation: Feasibility, performance and effect on laboratory quality assessment - A technical note

Nested PCR in lung tissue for diagnosis of pulmonary tuberculosis

Potent in vitro and in vivo antitubercular activity of certain newly synthesized indophenazine 1,3,5-trisubstituted pyrazoline derivatives bearing benzofuran

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