Comparative study on the utilization of crystalline methionine and methionine hydroxy analogue calcium by Pacific white shrimp (Litopenaeus vannamei Boone)

Abstract

The objective of this study was to compare the supplemental effects of crystalline DLmethionine (DL-Met) and methionine hydroxy analogue calcium (MHA-Ca) on growth performance of Pacific white shrimp. Eight isoproteinic (355.3 ± 2.0 g/kg diet) and isolipidic (70.0 ± 2.2 g/kg diet) diets were prepared as positive diet (20% fish meal), negative diet (15% fish meal) and DL-Met, MHA-Ca-supplemented diets with the supplementation of 0.03%, 0.06%, 0.09% DL-Met and 0.04%, 0.07%, 0.1% MHA-Ca in negative diet respectively. Pacific white shrimp (0.92 ± 0.03 g) were fed one of the eight diets for 49 days. The results showed that dietary DL-Met did not affect weight gain (WG) and feed conversion ratio (FCR) (p > 0.05), but the supplementation of 0.1% MHA-Ca significantly increased WG, protein and lipid retention, and reduced FCR (p < 0.05) when compared to the negative group, and reached the similar levels as the positive control. The total free amino acids (TFAA) in haemolymph of MHA-Ca groups and PC, NC group peaked at the 3rd hr after feeding, but the peaking time of DL-Met groups was advanced to the 2nd hr. Activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in haemolymph of all MHA-Ca groups peaked at the 2nd hr, but DL-Met groups peaked at the 1st hr after feeding. The above results indicated that the supplementation of 0.1% MHA-Ca in a low fish meal diet could improve the growth performance and feed utilization of Pacific white shrimp, but dietary DL-Met did not significantly affect the growth.

Optimal dietary methionine requirement for juvenile sea cucumber Apostichopus japonicus selenka

“…It is a substrate for protein synthesis; meanwhile, it also serves as the major methyl group donor for synthesis of polyamines, methylation of phospholipids, and DNA and RNA intermediates in vivo (Loy & Lundy, 2019). Usually, methionine was the most limiting amino acid in aquatic diet, especially in a feed with high proportion of legume protein (Chen et al., 2018). Many studies have been reported on the methionine requirements of marine aquatic species, which illustrated that dietary optimal methionine varied from 0.72% ( Litopenaeus vannamei ) (Felipe et al., 2016) to 2.58% ( Pseudosciaena crocea R.) (Yu et al., 2013).…”

Application of meta‐analysis towards understanding the effect of adding a methionine hydroxy analogue in the diet on growth performance and feed utilization of fish and shrimp

“…Jian carp and grass carp both belong to carp). The complete data set included 249 effect sizes ( n = 54 for FW, n = 67 for WG, n = 43 for PER, n = 52 for FCR, n = 33 for FE) from 23 studies that included eight different fish species including carp (Shen et al 2007; Jia 2010; Li 2010; Xiao et al 2011; Shan et al 2015; Pan et al 2016), channel catfish (Zhao et al 2017), red drum (Goff & Gatlin 2004), sea bass (Keembiyehetty & Gatlin III 1995; Keembiyehetty & Gatlin 1997; Kelly 2005; Kelly et al 2006; Savolainen & Gatlin III 2010; Zhang et al 2017), rainbow trout (Poston 1986; Cheng et al 2003), Nile tilapia (Zhao et al 2010), large yellow croaker (Ma et al 2016), and turbot (Ma et al 2013; Hu et al 2015), and one shrimp species, the Pacific white shrimp (Forster & Dominy 2006; Browdy et al 2012; Chen et al 2018).…”

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei)

“…Studies have demonstrated that methionine deficiency may lead to oxidative stress, liver damage and growth retardation in animals ( Bin et al, 2017 ; Lee et al, 2019 ). Chen et al (2018) reported that methionine deficiency led to lower weight gain rate, protein retention efficiency and higher feed conversion rate of L. vannamei when 25% fishmeal was replaced with soybean protein concentrate. As such, to cover the methionine requirements of animals, the exploration of methionine supplementation under low-fishmeal aquafeeds is being extensively researched.…”

“…The application effects of crystalline methionine and analogue products are still controversial in aquaculture. Some studies have shown that these methionine products are similar in their ability to satisfy the qualitative requirements of aquatic species without adverse effects ( Goff and Gatlin, 2004 ; Forster and Dominy, 2006 ; Zhou et al, 2018 ), while others have indicated that analogue products supplementation was not comparable with crystalline methionine ( Keembiyehetty and Gatlin, 1995 ; Zhao et al, 2017 ; Chen et al, 2018 ).…”