Comparative Study on Total Lipid Determination using Soxhlet, Roese-Gottlieb, Bligh &amp; Dyer, and Modified Bligh &amp; Dyer Extraction Methods

Manirakiza, P.; Covaci, Adrian; Schepens, Paul

doi:10.1006/jfca.2000.0972

Cited by 301 publications

(169 citation statements)

References 10 publications

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“…The total intracellular lipids were determined by the Soxhlet method (21). Fat was extracted using a Büchi B-811 universal extraction system (Büchi Labortechnic AG, Flawil, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Rakicka

Mirończuk

Tomaszewska-Hetman

et al. 2017

Food Technol. Biotechnol.

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SummaryThis study demonstrates the potential applicability of the UV mutant Yarrowia lipolytica MK1 for the valorisation of glycerol and erythritol production in a chemostat culture. The aim of this research is to investigate the optimal C:N ratio in the feeding medium in order to enhance erythritol production. The highest erythritol concentration, at 113.1 g/L with a volumetric erythritol production rate of 1.1 g/(L·h) and a yield of 0.57 g/g, was obtained in the feeding medium with a C:N ratio of 80:1. Moreover, no residual glycerol was observed in the culture broth during cultivation. The chemical composition of the biomass was analysed. The contents of lysine and threonine in the biomass protein amino acid profi le were higher than those required by the FAO/WHO for fodder yeast.

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Section: Methodsmentioning

confidence: 99%

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Rakicka

Mirończuk

Tomaszewska-Hetman

et al. 2017

Food Technol. Biotechnol.

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show abstract

“…The mixture was evaporated with rotary evaporator and 100 µL internal standard (Pentachloronitrobenzene) having a concentration of 0.5 µg/L was added. The final solution was pre-concentrated to 1 mL with a minivap evaporator under pure nitrogen gas and taken into a 2 ml amber glass vial and kept in refrigerator at 4°C before the analysis with GC-MS [27].…”

Section: Analytical Proceduresmentioning

confidence: 99%

Determination of Organochlorine Pesticides in Sediments Using Gas Chromatography and Mass Spectrometry

Km¹,

Gokmen²,

G³

2017

J Anal Bioanal Tech

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“…Milk fat matter was extracted from the thawed samples by the method described by Bligh & Dyer [27] modified by Manirakiza et al [28]. Briefly, 1 mL of each sample are transferred in a 15 mL Falcon test tube and 3.75 mL of a chloroform: methanol 1:2 solution are added.…”

Section: Milk Fat Determination and Fatty Acid Profilesmentioning

confidence: 99%

A Survey on Mono-, Polyunsaturated Fatty Acids, Desaturase Indices and Atherogenic Index in the Milk Fat of Local Breeds (Cabannina, Varzese and Valdostana) Reared in Northern Italy

Faustini¹,

Lm²,

Munari³

et al. 2016

JDVAR

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Comparative Study on Total Lipid Determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and Modified Bligh & Dyer Extraction Methods

Cited by 301 publications

References 10 publications

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Determination of Organochlorine Pesticides in Sediments Using Gas Chromatography and Mass Spectrometry

A Survey on Mono-, Polyunsaturated Fatty Acids, Desaturase Indices and Atherogenic Index in the Milk Fat of Local Breeds (Cabannina, Varzese and Valdostana) Reared in Northern Italy

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