Comparative study using Papanicolaou stain and silver-stained nucleolar organizer region counts in exfoliative smear of oral mucosa in bidi smokers and nonsmokers

Mhaske, Sheetal Prabhakar; Pattanshetti, Kirti; Jagtap, Kiran; Debta, Priyanka; Misurya, Archana L; Patel, Jaladhi H

doi:10.4103/jispcd.jispcd_65_18

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The slides were immersed in 1Mol hydrochloric acid (HCl) at 60°C for 5–6 min, followed by placement in 1Mol HCl at room temperature for 1 min and were then immersed in Schiff reagent for 30 min and washed under running tap water for 10 min. [ 16 17 18 19 20 21 22 23 ]…”

Section: Ethodologymentioning

confidence: 99%

Genotoxic Effect of Various forms of Tobacco on Oral Buccal Mucosa and Nuclear Changes as a biomarker

Devadoss

Raveendranath

Kathiresan

et al. 2021

Journal of Pharmacy and Bioallied Sciences

View full text Add to dashboard Cite

Aim: The present study aims to assess the genotoxic effect of various forms of tobacco users on buccal mucosa and nuclear changes as biomarkers. Materials and Methods: The study involves 150 cases, they were divided into three groups (two study groups and one control group). The buccal cytological smears were taken from three groups: Group I – 50 smokers, Group II – 50 nonsmokers (smokeless tobacco), and Group III – 50 control group. The buccal cells were transferred into a test tube containing Tris-ethylenediaminetetraacetic acid buffer (pH = 7) and was centrifuged (Remi® 1500 revolution/min [rpm]). Cell suspensions were transferred to the slides and fixed. The slides were stained using PAP and Feulgen stain. The MN and other nuclear abnormalities were studied and compared. Results: Nonsmokers (smokeless tobacco) had significantly increased frequency of all nuclear anomalies compared to smokers and healthy controls. Binucleation, karyorrhexis, micronuclei (MN), karyolysis, broken egg nuclei, and prominent nucleoli in nonsmokers (smokeless tobacco) and condensed chromatin in smokers were the most frequent anomalies. Binucleation and karyorrhexis were significantly more frequent in nonsmokers (smokeless tobacco) compared to smokers. The other nuclear abnormalities were not statistically significant in smokers and nonsmokers (smokeless tobacco). Conclusion: Numerous studies have stated that MN and other nuclear anomalies were present in higher frequency in smokers and nonsmokers. In our study, we found binucleation and karyorrhexis were statistically significant in nonsmokers (smokeless tobacco) compared to smokers. The other nuclear anomalies showed insignificant results. In order to further validate the significance of this study, a larger sample size has to be studied. On comparing the staining efficacy of smokers and nonsmokers using PAP and Feulgen stain, both the stains showed positive results. In the present study, DNA-specific Feulgen stain shows better staining of nuclear anomalies compared to DNA nonspecific PAP stain, which was found to be statistically significant.

show abstract

Section: Ethodologymentioning

confidence: 99%

Genotoxic Effect of Various forms of Tobacco on Oral Buccal Mucosa and Nuclear Changes as a biomarker

Devadoss

Raveendranath

Kathiresan

et al. 2021

Journal of Pharmacy and Bioallied Sciences

View full text Add to dashboard Cite

show abstract

Modern view on mechanisms of epithelium differentiation of the oral mucosa in normal and pathological processes

Hasiuk¹,

Bozhyk²,

Radchuk³

2021

Acta stomatologica Naissi

View full text Add to dashboard Cite

Background:In everyday clinical practice, patients who seek dental care in case of oral mucosa diseases are one of the most difficult categories of patients due to difficulties in diagnosis and treatment. Materials and methods: The review and analysis of scientific and medical literature based on the Scopus, Web of Science, MedLine, PubMed, NCBI databases, the study of which does not exceed 5 years, including literature reviews and the results of clinical trials. Results: Changes in the oral mucosa can be clearly specific. Clinicians can make the correct diagnosis and determine the tactics of treatment by appearance. However, in most cases the diagnosis of diseases with this anatomical location is complicated, because the clinical picture is nonspecific and often burdened with additional local and general adverse factors. A detailed clinical examination and additional research methods are required to establish the correct diagnosis. Diagnosis of oral mucosa diseases is based on a careful assessment of clinical and laboratory data. Conclusions: Taking into account the prevalence of diseases of the oral mucosa, of particular interest is the question of the course of the process of differentiation of the epithelium of various anatomical zones in the norm and the mechanisms of restructuring of the cellular composition in pathological processes, with the cops of objectifying the diagnosis, predicting the course of the disease and pathogenetically substantiated treatment.

show abstract

Comparative study using Papanicolaou stain and silver-stained nucleolar organizer region counts in exfoliative smear of oral mucosa in bidi smokers and nonsmokers

Cited by 2 publications

References 12 publications

Genotoxic Effect of Various forms of Tobacco on Oral Buccal Mucosa and Nuclear Changes as a biomarker

Genotoxic Effect of Various forms of Tobacco on Oral Buccal Mucosa and Nuclear Changes as a biomarker

Modern view on mechanisms of epithelium differentiation of the oral mucosa in normal and pathological processes

Contact Info

Product

Resources

About