Comparative Survival of Human Spermatozoa in Media Supplemented With Polyvinyl Alcohol or Human Serum Albumin

Javed, Murid; Geisman, M; Shaikh, Mhejabeen; Ruberto, C; Valle, A.P. Del

doi:10.1016/s0015-0282(00)01450-3

Cited by 2 publications

(1 citation statement)

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“…Similar effects were also observed in hamster spermatozoa by adding PVA into sperm in in vitro culture media without any effect on sperm acrosome reaction, in vitro fertilization or embryo development (Bavister, ). Javed, Geisman, Shaikh, Ruberto, and Valle () used PVA as a substitute for human serum albumin (HSA) in human sperm cryopreservation extender. The PVA provided comparable sperm survival and kinematics, proved to be non‐toxic to human spermatozoa and could be a suitable replacement for HSA.…”

Section: Discussionmentioning

confidence: 99%

Use of polyvinyl alcohol as a chemically defined compound in egg yolk‐free extender for dog sperm cryopreservation

Nabeel

Jeon

2019

Reprod Domestic Animals

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The objectives of this study were to investigate the effects of polyvinyl alcohol (PVA) as a chemically defined compound in egg yolk (EY)‐free extender by determining the appropriate concentration of PVA and the effect of pH adjustment in EY‐free PVA extenders on dog spermatozoa. Spermatozoa (1 × 108 cells/ml) were frozen with EY‐free extenders supplemented with 0 (control), 0.025, 0.05, 0.1, 0.2 or 0.3 g/100 ml PVA. Sperm progressive motility (PM) was assessed immediately after thawing (IAT) and post‐thaw incubation (PTI), while viability, acrosome integrity and reactive oxygen species (ROS) levels were evaluated after PTI. Additionally, spermatozoa were frozen using EY‐free PVA extenders before pH adjustment (6.45) and after adjustment of pH (6.85). Viability, PM, ROS and gene expression (BCL2 and SMCP) were assessed. Supplementation with 0.05 g/100 ml or more PVA significantly increased PM compared to the control group in the IAT and PTI. Post‐thaw incubation significantly increased sperm motility in all groups. The acrosome integrity in all PVA groups was higher (p < .05) than the control without an effect on ROS and viability. Adjustment of the pH to 6.85 improved (p < .05) sperm PM compared to the non‐adjusted groups without affecting viability, ROS or expression of BCL2 and SMCP. We suggest that PVA supplementation in EY‐free Tris extenders can effectively protect dog spermatozoa during freezing and can maintain higher motility and acrosome integrity. Adjustment of pH in EY‐free PVA extenders can improve post‐thaw sperm motility. Therefore, PVA can be used as a compound in EY‐free extender for the cryopreservation of dog spermatozoa.

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Section: Discussionmentioning

confidence: 99%