Comparative susceptibility of carp fingerlings to Lernaea cyprinacea infection

Hemaprasanth,; Singh, Ravinder; Raghavendra, A.; Nimmagadda, Sridhar; Raghunath, M.R.; Ambekar, Jalindar D.

doi:10.1016/j.vetpar.2010.12.029

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…Objective histopathological findings in our study confirmed that the invasion of the parasitic crustacean L. cyprinacea is capable of causing a wide range of microscopic lesions, including hemorrhage and the aggregation and infiltration of inflammatory cells in the host, which were consistent with the findings of Singh et al 65 , Hemaprasanth et al 66 , Pala et al 17 , and Furtado et al 67 . Furthermore, parasitic granulomatous inflammation was observed, which agreed with the findings of Furtado et al 67 .…”

Section: Discussionsupporting

confidence: 91%

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

Rahmati-Holasoo,

Marandi,

Shokrpoor

et al. 2023

Sci Rep

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Parasitic infestations are one of the most concerning problems limiting ornamental fish farming. In addition to the direct economic losses due to the major mortalities, parasites may significantly negatively impact the body shape, coloration, growth rate, and health condition of the fish. The results of the study highlight the importance of comprehensive parasitological analysis in the diagnosis and treatment of parasitic infections in ornamental fish farms. The presence of multiple parasites in the affected fish emphasizes the need for effective biosecurity measures, such as extending the quarantine period for newly imported fish, closely monitoring fish populations, and implementing isolation units to prevent the spread of infections. By implementing these preventative measures, ornamental fish farmers can reduce the risk of parasitic infections and ensure the health and well-being of their fish populations. This, in turn, can lead to increased profitability and sustainability for their business. Overall, the current study aimed to conduct a clinical, histopathological, and phylogenetic analysis of the epibiont ciliated protozoan Epistylis wuhanensis and the copepod crustacean Lernaea cyprinacea in a freshwater ornamental fish farm in Iran. Furthermore, it provides valuable insights into the prevalence and impact of parasitic infections in ornamental fish farms and underscores the need for continued research and the development of effective preventative measures to address this issue. A total of 60 symptomatic freshwater ornamental fish, including 30 guppy (Poecilia reticulata) and 30 sailfin molly (Poecilia latipinna), were packed in polyethylene bags filled with oxygenated pond water and transported to the Ornamental Fish Clinic, Faculty of Veterinary Medicine, University of Tehran, for parasitological analysis. Following the clinical examination, histopathological analysis was performed on 10% NBF (neutral buffered formalin)-fixed samples from affected tissues, including the skin, skeletal muscle, and liver, to identify any pathological changes associated with the parasitic infections. Furthermore, the DNA was extracted from the 99% ethanol-fixed samples using a commercial DNA extraction tissue kit (SinaPure DNA, Iran), and PCR was performed using Peri18S-F1 (5′-ACC TGG TTG ATC CTG CCA GT-3′) and Peri18S-R1 (5′-TGC AGG TTC ACC TAC GGA AA-3′) (first reaction), and Peri18S-F2 (5′-CCG CGG TAA TTC CAG CTC-3′) and Peri18S-R2 (5′-GAT CCC CTA ACT TTC GTT CTT GA-3′) (second round) primers for the identified parasites. Finally, the PCR products were sequenced using Sanger dideoxy sequencing methods, and the resulting sequences were compared to sequences in the BLAST search program to provide a comprehensive picture of the current parasite-based disorder. The crustacean L. cyprinacea and the epibiont sessilid E. wuhanensis were identified in the examined ornamental guppy (6/30) and sailfin molly (6/30), with an overall parasitic prevalence of 20.00% (12/60). Ciliates were found in all tissue lesions but not in fish without lesions. A great number of the ciliated protozoan E. wuhanensis were found attached to the integumentary area of L. cyprinacea. Microscopically, oval to round granulomatous lesions were observed in cutaneous and skeletal muscles. Lymphoplasmacytic dermatitis and myositis were also observed. The crustacean L. cyprinacea serves as a mechanical vector for E. wuhanensis infection and spreads the disease in ornamental fish farming operations. For the first time in Iran, we successfully presented diagnostic morphological and molecular data on sessilids isolated from L. cyprinacea. Based on the findings of the current study, such parasitic infections may cause significant economic losses following invasion of the integument area of fish, eventually leading to death, if treatment is neglected or inadequate. Furthermore, the findings of the analysis were used to develop effective diagnostic approaches for the affected fish, as well as recommendations for improved health conditions to prevent future outbreaks of parasitic infections. However, further research is needed to determine the precise mechanisms of crustacean attachment and host-crustacean-peritrich protozoan interactions. Furthermore, the direct and indirect effects of various environmental factors on the emergence and spread of the current disease should be considered.

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Section: Discussionsupporting

confidence: 91%

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

Rahmati-Holasoo,

Marandi,

Shokrpoor

et al. 2023

Sci Rep

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“…In central Argentina, the spread of the copepod is related to the fishing season, when thousands of small fish are sold as bait and transported to different environments (Mancini et al 2008a). Thus larval stages can be transported away through water resulting in spread of L. cyprinacea (Mancini et al 2008a, Hemaprasanth et al 2011. No parasitological studies have been done upstream Los Barreales Reservoir; therefore further studies are needed to determine the current distribution of L. cyprinacea in Neuquén River Basin.…”

Section: Discussionmentioning

confidence: 99%

The invasive copepod Lernaea cyprinacea Linnaeus, 1758 (Copepoda, Cyclopoida, Lernaeidae): first record for Neuquén River,Patagonia, Argentina

Waicheim¹,

Blasetti²,

Rauque³

et al. 2017

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We report the first record of the copepod Lernaea cyprinacea (Linnaeus, 1758) parasitizing Percichthys trucha (Cuvier & Valenciennes, 1840) in the Neuquén River (Patagonia, Argentina). One specimen of P. trucha was found parasitized by two females of L. cyprinacea. Our record of L. cyprinacea in Neuquén River represents an increase of the known distribution range for this invasive species, which is apparently moving upstream in the Negro River Basin. This is the first record of L. cyprinacea in Neuquén Province.

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“…They can manifest differently, in particular, pathological changes in the body can lead to death, latent disease, growth and development delay and decreased productivity of fish (Vovk, 2002;Rastiannasab et al, 2016;Golzio et al, 2017). One of the reasons hampering the development of fish farming and increasing fish productivity is ectoparasitic because of these fish diseases (Hemaprasanth et al, 2011;Loboiko, 2012;Benovics et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The natural forage base of ponds and its influence on the development of ectoparasitic invasions in the fish farms of the Lviv region, Ukraine

Yurii¹,

Yevheniia²,

Bohdan³

et al. 2020

Egypt. J. of Aquatic Biolo. and Fish.

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Comparative susceptibility of carp fingerlings to Lernaea cyprinacea infection

Cited by 11 publications

References 16 publications

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

The invasive copepod Lernaea cyprinacea Linnaeus, 1758 (Copepoda, Cyclopoida, Lernaeidae): first record for Neuquén River,Patagonia, Argentina

The natural forage base of ponds and its influence on the development of ectoparasitic invasions in the fish farms of the Lviv region, Ukraine

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Comparative susceptibility of carp fingerlings to Lernaea cyprinacea infection

Cited by 11 publications

References 16 publications

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

Clinico-histopathological and phylogenetic analysis of protozoan epibiont Epistylis wuhanensis associated with crustacean parasite Lernaea cyprinacea from ornamental fish in Iran

The invasive copepod Lernaea cyprinacea Linnaeus, 1758&nbsp;(Copepoda, Cyclopoida, Lernaeidae): first record for Neuqu&eacute;n River,Patagonia, Argentina

The natural forage base of ponds and its influence on the development of ectoparasitic invasions in the fish farms of the Lviv region, Ukraine

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The invasive copepod Lernaea cyprinacea Linnaeus, 1758 (Copepoda, Cyclopoida, Lernaeidae): first record for Neuquén River,Patagonia, Argentina