Comparative Susceptibility of Eight Mosquito Species to Sindbis Virus *

B, Schiefer; Smith, James

doi:10.4269/ajtmh.1974.23.131

Cited by 10 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, SINV has been isolated from Aedine, Mansonia and Anopheles mosquito species in nature [ 5 , 6 ] and demonstrated to replicate in Aedes aegypti and Ae. albopictus in the laboratory setting [ 7 ]. Replication of arboviruses in the mosquito host is essential for virus persistence, and a horizontal cycle is the primary mechanism of Alphavirus maintenance in nature [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts

Ciano

Saredy

Bowers

2014

Viruses

View full text Add to dashboard Cite

Variants of the prototype Alphavirus, Sindbis (SINV), were used in per os infections of adult female mosquitoes to investigate arbovirus interaction with the salivary gland (SG). Infection of Aedine mosquitoes with AR339, a heparan sulfate proteoglycan (HSPG)-dependent variant, resulted in gross pathology in the SG lateral lobes while infection with TR339, a HSPG-independent variant, resulted in minimal SG pathology. HSPG was detected in the internal ducts of the SG lateral lobes by immunolabeling but not in the median lobe, or beyond the triad structure and external ducts. Reports that human lactoferrin interacts with HSPG, suggested an interference with virus attachment to receptors on vertebrate cells. Pre-incubation of Aedes albopictus cultured C7-10 cells with bovine lactoferrin (bLF) followed by adsorption of SINV resulted in earlier and greater intensity of cytopathic response to TR339 compared with AR339. Following pre-treatment of C7-10 cells with bLF, plaques from tissue culture-adapted high-titer SINVTaV-GFP-TC were observed at 48 h post-infection (p.i.), while plaques from low-titer SINVTaV-GFP-TC were not observed until 120 h p.i. Confocal optics detected this reporter virus at 30 days p.i. in the SG proximal lateral lobe, a region of HSPG-immunolocalization. Altogether these data suggest an association between SINV and HSPG in the host mosquito.

show abstract

Section: Introductionmentioning

confidence: 99%

Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts

Ciano

Saredy

Bowers

2014

Viruses

View full text Add to dashboard Cite

show abstract

“…In addition to these major arboviruses, Cx. tritaeniorhynchus has also been implicated (through laboratory experiments or field studies), as a competent or potential vector of: Sindbis virus (29) and Getah virus (10,30) (Family: Togaviridae , Genus: Alphavirus ); Bagaza virus (31,32) and Tembusu virus (33) (Genus: Flavivirus ); Batai (Chittoor) virus (34), Manzanilla virus (35), Cat Que virus (36), and Akabane virus (37) (Family: Peribunyaviridae , Genus: Orthobunyavirus ); Banna virus (38) (Family: Reoviridae , Genus: Seadornavirus ); Mengovirus (Cardiovirus A) (8) (Family: Picornaviridae , Genus: Cardiovirus ); Chandipura virus (39) (Family: Rhabdoviridae , Genus: Vesiculovirus ); and the filarial nematode parasite Dirofilaria immitis (40).…”

Section: Introductionmentioning

confidence: 99%

Genetic and microbial diversity of the invasive mosquito vector speciesCulex tritaeniorhynchusacross its extensive inter-continental geographic range

Jeffries

Tantely

Kadriaj

et al. 2022

Preprint

View full text Add to dashboard Cite

Culex (Cx.) tritaeniorhynchus is a mosquito species with an extensive and expanding inter-continental geographic distribution, currently reported in over 50 countries, across Asia, Africa, the Middle East, Europe and now Australia. It is an important vector of medical and veterinary concern, capable of transmitting multiple arboviruses which cause significant morbidity and mortality in human and animal populations. In regions endemic for Japanese encephalitis virus (JEV) in Asia, Cx. tritaeniorhynchus is considered the major vector and this species has also been shown to contribute to the transmission of several other significant zoonotic arboviruses, including Rift Valley fever virus and West Nile virus. Significant variation in vectorial capacity can occur between different vector populations. Obtaining knowledge of a species from across its geographic range is crucial to understanding its significance for pathogen transmission across diverse environments and localities. Vectorial capacity can be influenced by factors including the mosquito genetic background, composition of the microbiota associated with the mosquito and the co-infection of human or animal pathogens. In addition to enhancing information on vector surveillance and potential risks for pathogen transmission, determining the genetic and microbial diversity of distinct populations of a vector species is also critical for the development and application of effective control strategies. In this study, multiple geographically dispersed populations of Cx. tritaeniorhynchus from countries within Europe, Africa, Eurasia and Asia were sampled. Molecular analysis demonstrated a high level of genetic and microbial diversity within and between populations, including genetic divergence in the mosquito CO1 gene, as well as diverse microbiomes identified by 16S rRNA gene amplicon sequencing. Evidence for the detection of the endosymbiotic bacteria Wolbachia in some populations was confirmed using Wolbachia-specific PCR detection and sequencing of Wolbachia MLST genes; in addition to PCR-based detection of insect-specific viruses. Laboratory vector competence showed Cx. tritaeniorhynchus from a Greek population are likely to be competent vectors of JEV. This study expands understanding of the diversity of Cx. tritaeniorhynchus across its inter-continental range, highlights the need for a greater focus on this invasive vector species and helps to inform potential future directions for development of vector control strategies.

show abstract

“…The mosquito Aedes ( Ae. ) aegypti , the Yellow Fever mosquito, well-known as an arbovirus-transmitting vector, is a vector of the Alphavirus Sindbis in the laboratory setting [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

The Alphavirus Sindbis Infects Enteroendocrine Cells in the Midgut of Aedes aegypti

Ahearn¹,

Saredy

Bowers

2020

Viruses

View full text Add to dashboard Cite

Transit of the arthropod-borne-virus (arbovirus) Sindbis (SINV) throughout adult female mosquitoes initiates with its attachment to the gut lumen, entry and amplification in midgut cells, followed by dissemination into the hemolymph. Free-mated adult females, aged day 5–7, were proffered a viremic blood suspension via sausage casings containing SINV-TaV-Green Fluorescent Protein (GFP) at a final titer of 106 PFU/mL. Midguts (MGs) from fully engorged mosquitoes were resected on days 5 and 7 post-bloodmeal, and immunolabeled using FMRFamide antibody against enteroendocrine cells (ECs) with a TX-Red secondary antibody. Following immunolabeling, the organs were investigated via laser confocal microscopy to identify the distribution of GFP and TX-Red. Infection using this reporter virus was observed as multiple GFP expression foci along the posterior midgut (PMG) epithelium and ECs were observed as TX-Red labeled cells scattered along the entire length of the MG. Our results demonstrated that SINVGFP did infect ECs, as indicated by the overlapping GFP and TX-Red channels shown as yellow in merged images. We propose that ECs may be involved in the SINV infection pathway in the mosquito MG. Due to the unique role that ECs have in the exocytosis of secretory granules from the MG and the apical-basolateral position of ECs in the PMG monolayer, we speculate that these cells may assist as a mechanism for arboviruses to cross the gut barriers. These findings suggest that MG ECs are involved in arbovirus infection of the invertebrate host.

show abstract

Comparative Susceptibility of Eight Mosquito Species to Sindbis Virus *

Cited by 10 publications

References 0 publications

Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts

Heparan Sulfate Proteoglycan: An Arbovirus Attachment Factor Integral to Mosquito Salivary Gland Ducts

Genetic and microbial diversity of the invasive mosquito vector speciesCulex tritaeniorhynchusacross its extensive inter-continental geographic range

The Alphavirus Sindbis Infects Enteroendocrine Cells in the Midgut of Aedes aegypti

Contact Info

Product

Resources

About