Comparative Susceptibility of Rabbits, Rats, Mice and Pigs to Infection with Aujeszky Disease Virus (ADV) in the Development of an Efficacy Test for ADV Vaccines

Jakubik, J.

doi:10.1111/j.1439-0450.1977.tb01051.x

Cited by 9 publications

(4 citation statements)

References 2 publications

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“…However, 33.3% (2/6) of the pigs did not develop a protective level of antibodies, indicating that pigs need to be administered the virus twice or more. It has been reported that the oral route required more Aujeszky diseases virus vaccine than the intranasal route [ 22 ]. Additionally, the presence of antibodies against RABV has been confirmed in wild boar living in Slovenia where anti-rabies vaccination occurred in 2005-2006 [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

A recombinant rabies virus (ERAGS) for use in a bait vaccine for swine

Yang

Kim

Choi

et al. 2016

Clin Exp Vaccine Res

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PurposeRabies viruses (RABV) circulating worldwide in various carnivores occasionally cause fatal encephalitis in swine. In this study, the safety and immunogenicity of a recombinant rabies virus, the ERAGS strain constructed with a reverse genetics system, was evaluated in domestic pigs.Materials and MethodsGrowing pigs were administered 1 mL (108.0 FAID50/mL) of the ERAGS strain via intramuscular (IM) or oral routes and were observed for 4 weeks' post-inoculation. Three sows were also inoculated with 1 mL of the ERAGS strain via the IM route. The safety and immunogenicity in swine were evaluated using daily observation and a virus-neutralizing assay (VNA). Fluorescent antibody tests (FAT) for the RABV antigen and reverse transcriptase-polymerase chain reaction (RT-PCR) assays for the detection of the nucleocapsid (N) gene of RABV were conducted with brain tissues from the sows after necropsy.ResultsThe growing pigs and sows administered the ERAGS strain did not exhibit any clinical sign of rabies during the test period test and did develop VNA titers. The growing pigs inoculated with the ERAGS strain via the IM route showed higher VNA titers than did those receiving oral administration. FAT and RT-PCR assays were unable to detect RABV in several tissues, including brain samples from the sows.ConclusionOur results suggest that the ERAGS strain was safe in growing pigs and sows and induced moderate VNA titers in pigs.

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Section: Discussionmentioning

confidence: 99%

A recombinant rabies virus (ERAGS) for use in a bait vaccine for swine

Yang

Kim

Choi

et al. 2016

Clin Exp Vaccine Res

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“…In addition, pigs vaccinated three times attained 100% seropositivity earlier (by 7 weeks after vaccination) than did pigs vaccinated twice. It was earlier reported that large quantities of ADV were necessary to adequately infect animals and that the oral route required more vaccine than did the intranasal route [ 17 ]. It may be that a large quantity of ADV is required to induce an adequate immune response in domestic pigs.…”

Section: Discussionmentioning

confidence: 99%

An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs

Yang

Kim

Choi

et al. 2016

Clin Exp Vaccine Res

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PurposeAujeszky's disease (AD) is an economically important disease affecting both wild and domestic pigs of the species Sus scrofa. A previous study yielded serological evidence of AD in Korean wild boars, which could spread AD to other animals. A new Aujeszky's disease virus (ADV) bait vaccine is required to prevent AD outbreaks in swine. In the present study, we investigated the safety and immunogenicity of a gE-deleted marker vaccine, strain YS-400, in young domestic pigs.Materials and MethodsThe YS-400 strain was propagated in Vero cells, and the trial ADV bait vaccine (a vaccine blister in a matrix including an attractant) was prepared. Pigs were orally immunized with the vaccine (2 mL, 107.5 TCID50/mL) delivered using a syringe or in the bait vaccine. The animals were observed for 9 weeks after vaccination, and immunogenicity was assessed using a virus neutralization (VN) test and enzyme linked immunosorbent assay.ResultsThe YS-400 strain was non-pathogenic to pigs when given orally and induced high VN titers (1:32-1:128) 6 weeks post-administration. Of the pigs given the ADV bait vaccine twice or three times, 40% were seropositive by 2 weeks, and 100% were seropositive by 7 weeks after the first dose. Pigs that consumed the AD bait vaccine three times developed VN titers that were slightly higher than those of pigs given the vaccine twice.ConclusionDomestic pigs given the trial ADV bait vaccine exhibited no adverse effects and developed high VN titers against ADV, indicating that the YS-400 strain is safe and can prevent ADV infection in domestic pigs.

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“…Although AD does not occur in laboratory animals under natural conditions, they are highly susceptible to experimental infection [ 111 ]. Rabbits were found to be the most sensitive species [ 112 ] and have been used for diagnostic purposes [ 113 ]. When subcutaneously inoculated, rabbits exhibit neurological deficits, hyperactivity, intense pruritus and self-mutilation at the site of inoculation [ 114 ].…”

Section: Aujeszky’s Disease In Mammalsmentioning

confidence: 99%

Comparative Pathology of Pseudorabies in Different Naturally and Experimentally Infected Species—A Review

Sehl

Teifke

2020

Pathogens

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The pseudorabies virus (PRV) is an alphaherpesvirus and the causative agent of Aujeszky’s disease (AD). PRV infects a wide range of animal species including swine as the natural host as well as ruminants, carnivores, rodents and lagomorphs. In these species, except for the pig, PRV infection causes acute, severe disease, characterized by insatiable itching, and is always lethal. Horses, chickens and non-human primates have been shown to be largely resistant to PRV infection, while disease in humans is still controversial. PRV is a pantropic virus, which preferably invades neural tissue, but also infects epithelia of various organs, whereupon multisystemic lesions may result. Although AD is mainly associated with severe pruritus, also known as “mad itch”, there are notable differences regarding infection route, clinical signs, viral distribution and lesion patterns in different animal species. In this comprehensive review, we will present clinico-pathologic findings from different species, which have been either shown to be susceptible to PRV infection or have been tested experimentally.

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Comparative Susceptibility of Rabbits, Rats, Mice and Pigs to Infection with Aujeszky Disease Virus (ADV) in the Development of an Efficacy Test for ADV Vaccines

Cited by 9 publications

References 2 publications

A recombinant rabies virus (ERAGS) for use in a bait vaccine for swine

A recombinant rabies virus (ERAGS) for use in a bait vaccine for swine

An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs

Comparative Pathology of Pseudorabies in Different Naturally and Experimentally Infected Species—A Review

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