Comparative toxicity evaluation of flower-shaped and spherical gold nanoparticles on human endothelial cells

Sultana, Sadequa; Djaker, Nadia; Boca, Sanda; Salerno, Milena; Charnaux, Nathalie; Aştilean, Simion; Hlawaty, Hanna; Chapelle, Marc Lamy de la

doi:10.1088/0957-4484/26/5/055101

Cited by 59 publications

(42 citation statements)

References 42 publications

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“…These data clearly suggest an original behavior of HIV‐1 Tat and gemcitabine in the nanoconjugated form retains its specific targeting ability expressing cancer cells in vitro . Because of their size (<10 nm), AuNPs showed more effect on cell proliferation at high concentration compared to complexes (size ≈ 50 nm), as already demonstrated by the authors in a previous work . All nanoparticles were “free” endotoxin and suitable for further nanomedicine applications.…”

Section: Resultssupporting

confidence: 77%

HIV‐1 Tat Peptide‐Gemcitabine Gold (III)‐PEGylated Complex—Nanoflowers: A Sleek Thermosensitive Hybrid Nanocarrier as Prospective Anticancer

Liu

Jiang

et al. 2018

Part & Part Syst Charact

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This paper focuses on the synthesis of novel metal‐drugs nanoparticles based onto gold‐gemcitabine complexes relevant for biomedical applications. Gold (HAuCl4) is mixed with antitumor compounds (gemcitabine (GEM)) and dicarboxylic acid–terminated polyethylene‐glycol (PEG), which plays a double role as surfactants and reducing agent. The proposed methodology is fast and reproducible and leads the formation of hybrid‐metal nanoflowers called GEM ION‐PEG‐AuNPs, in which drug solubility and efficiency are improved. The emerging role of GEM ION PEG‐AuNPs in the hyperthermic treatment was applied in pancreatic cancer cells under specific experimental conditions. For this purpose GEM ION‐PEG‐AuNPs are decorated with HIV‐1 Tat peptide, a potent trans‐activator of transcription from the viral promoter and is essential for viral replication containing an activation domain and a nucleic acid‐binding domain. All products are evaluated by various spectroscopic techniques (Raman and UV–vis) and transmission electron microscopy (TEM). This study, revealing an original chemical approach, will employ an important position in the field of nanomedicine, with novel perspectives that will be proposed for the development of new drugs and active targeting in cancer therapy.

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Section: Resultssupporting

confidence: 77%

HIV‐1 Tat Peptide‐Gemcitabine Gold (III)‐PEGylated Complex—Nanoflowers: A Sleek Thermosensitive Hybrid Nanocarrier as Prospective Anticancer

Liu

Jiang

et al. 2018

Part & Part Syst Charact

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“…Our TEM analysis indicated that the pAuNPs could be detected at the cell peripheries and in the cytoplasm of VSMCs at an early stage of treatment ( Figure 2B), suggesting that the pAuNPs had entered VSMCs. According to a previous report 40 and the models suggested by Sultana et al, 41 internalized AuNPs can be transported, transformed, and ultimately liberated into the cytoplasm, leading to local accumulation and alteration of physiological functions of cells. Therefore, we can conclude that the endocytosed pAuNPs actually compromise VSMC functions.…”

Section: Discussionmentioning

confidence: 91%

Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells

Lo¹,

Ma²,

Shieh³

et al. 2018

IJN

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IntroductionVascular smooth muscle cells (VSMCs) play an important role in the development and progression of atherosclerosis and vascular injuries in terms of proliferation and migration. Therefore, the aim of this study was to investigate the anti-migratory and proliferative effects of naked gold nanoparticles (AuNPs) on VSMCs.Materials and methodsOne set of physically synthesized AuNPs (pAuNPs) and three sets of chemically synthesized AuNPs (cAuNPs) were tested.Results and discussionAmong them, the pAuNPs were found to significantly and markedly inhibit platelet-derived growth factor (PDGF)-induced VSMC migration. Transmission electron microscopy revealed that the pAuNPs were ingested and aggregated in the cytoplasm at an early stage of treatment, while the viability of VSMCs was not affected within 24 hours of treatment. The pAuNP treatment enhanced cellular mitochondrial activity but inhibited basal and PDGF-induced VSMC proliferation, as determined by MTT, WST-1, and BrdU cell proliferation assays. Furthermore, the pAuNPs did not interfere with PDGF signaling or matrix metalloproteinase-2 expression/activity. Unlike the cAuNPs, the pAuNPs could markedly reduce VSMC adhesion to collagen, which was supported by the findings that the pAuNPs could inhibit collagen-induced tyrosine protein and focal adhesion kinase (FAK) phosphorylation and actin cytoskeleton reorganization during cell adhesion. The in vitro effects of the pAuNPs were confirmed in the in vivo rat balloon-injured carotid artery model by diminishing the proliferating VSMCs.ConclusionTaken together, the present study provides the first evidence that naked pAuNPs can reduce VSMC migration and compromise cell adhesion by affecting FAK and tyrosine-protein activation. The pAuNPs also have an inhibitory effect on PDGF-induced VSMC proliferation and can reduce proliferating/migrating VSMC expression in vivo.

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“…There have therefore been studies focusing on the cytotoxic potential of NPs themselves. These papers indicate that AuNPs have different toxicities in cells depending on concentration and shape, and spherical AuNPs at low concentrations have no or weak cytotoxicity against endothelial cells (19)(20)(21)(22). Indeed, cytotoxicity has been identified to be influenced by NP properties including size and surface chemistry (19,20).…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles enhance X‑ray irradiation‑induced apoptosis in head and neck squamous cell carcinoma in�vitro

et al. 2018

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Enhancing the antitumor effect of radiation, while reducing damage to organs, is a significant challenge in radiation therapy for head and neck malignancies. One promising radiosensitizer is gold. The present study aimed to determine whether gold nanoparticles (AuNPs) have the potential to enhance the effects of X-ray irradiation on head and neck cancer cells. The human head and neck carcinoma cell line HSC-3 was used. Total cell number and the levels of cell proliferation and apoptosis were compared between control cells and cells treated with 5-nm AuNPs alone at four concentrations (0.1, 0.4, 1.0 and 10.0 nM), X-ray irradiation alone at three doses (2, 4 and 8 Gy), or a combination of 4 Gy X-ray irradiation and 1.0 nM AuNPs. Analysis of variance and Tukey-Kramer testing were performed to compare the different groups. The total number of cells significantly decreased following 4 and 8 Gy X-ray irradiation, compared with in the control group (control vs. 4Gy, P=2.19x10-4 ; control vs. 8Gy, P=1.28x10-6). The combination of 4 Gy X-ray irradiation and 1.0 nM AuNPs significantly reduced the total number of cells compared with 4 Gy X-ray irradiation alone (P=2.95x10-4). Cell proliferation was not affected by AuNP treatment alone, 4 Gy X-ray irradiation alone or the combination of X-ray irradiation and AuNPs. The combination of 4 Gy irradiation and 1.0 nM AuNPs significantly increased the number of apoptotic cells compared with 4 Gy irradiation alone (P=0.0261). In conclusion, AuNPs combined with X-ray irradiation enhanced the cytotoxic effect on human head and neck cancer cells in vitro, through the induction of apoptosis, but not inhibition of cell proliferation.

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Comparative toxicity evaluation of flower-shaped and spherical gold nanoparticles on human endothelial cells

Cited by 59 publications

References 42 publications

HIV‐1 Tat Peptide‐Gemcitabine Gold (III)‐PEGylated Complex—Nanoflowers: A Sleek Thermosensitive Hybrid Nanocarrier as Prospective Anticancer

HIV‐1 Tat Peptide‐Gemcitabine Gold (III)‐PEGylated Complex—Nanoflowers: A Sleek Thermosensitive Hybrid Nanocarrier as Prospective Anticancer

Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells

Gold nanoparticles enhance X‑ray irradiation‑induced apoptosis in head and neck squamous cell carcinoma in�vitro

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