Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes

Adashev, Vladimir E; Bazylev, Sergei S.; Potashnikova, Daria; Godneeva, Baira; Шацких, А. С.; Olenkina, Oxana M.; Olenina, Ludmila V.; Kotov, A. A.

doi:10.1016/j.ejcb.2022.151246

Cited by 5 publications

(4 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aubergine is part of the argonaut family in Drosophila and is involved in gene silencing in the germline 34 indicating a potential involvement in MSCI starting in spermatocyte cells. Additionally, the RacGAP1/tumbleweed gene (AGAP008912), expressed in the ring canals of pre-meiotic germ cells to permit cytoplasmic sharing [35][36][37] , showed enrichment in the GSCs (Log2FC: 2.83, p = 0.005), Primary spermatogonia (Log2FC: 2.61, p < 0.0001) and Spermatogonia/Primary spermatocyte (Log2FC: 1.90, p < 0.0001) cell clusters, con rming their pre-meiotic cell state.…”

Section: Resultsmentioning

confidence: 99%

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Page

Galizi

Taxiarchi

et al. 2023

Preprint

View full text Add to dashboard Cite

Understanding of the Anopheles gambiae germline has been essential to acquiring effective genetic control strategies targeting this malaria mosquito vector. This includes targeting germline genes to induce sterility or using regulatory sequences to drive transgene expression for applications such as gene drive. However, only very few germline regulatory elements have been characterised with the majority showing leaky expression. This has been shown to significantly reduce the efficiency of current genetic control strategies, which rely on regulatory elements that are more tightly restricted in their spatial and/or temporal expression. Additionally, meiotic silencing of the sex chromosomes during spermatogenesis limits the flexibility of transgene expression for design of novel genetic control strategies. In this work, we build on our previous study that dissected gametogenesis into four distinct cell populations. We used single-cell RNA sequencing to further discriminate these populations and define distinct germline cell-types. In doing so, we revealed an overexpression of X-linked genes in the germline stem cells (GSCs) and were able to pinpoint the onset of meiotic silencing of the X chromosome in the spermatogonia/primary spermatocytes. This study provides a comprehensive dataset to identify genes expressed at specific stages of spermatogenesis, widening the toolkit for genetic control of malaria mosquitoes.

show abstract

Section: Resultsmentioning

confidence: 99%

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Page

Galizi

Taxiarchi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…These genes, related to the “mitotic cell cycle process”, exhibited analogous expression patterns in the testes of both species ( Table S10 ). Intriguingly, this list includes homologs of Bub1 kinase [ 52 ] and tumbleweed [ 53 ], each with well-established roles in D. melanogaster spermatogenesis.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori

Kakino

Mon

Ebihara

et al. 2023

Insects

View full text Add to dashboard Cite

Spermatogenesis is an important process in reproduction and is conserved across species, but in Bombyx mori, it shows peculiarities, such as the maintenance of spermatogonia by apical cells and fertilization by dimorphic spermatozoa. In this study, we attempted to characterize the genes expressed in the testis of B. mori, focusing on aspects of expression patterns and gene function by transcriptome comparisons between different tissues, internal testis regions, and Drosophila melanogaster. The transcriptome analysis of 12 tissues of B. mori, including those of testis, revealed the widespread gene expression of 20,962 genes and 1705 testis-specific genes. A comparative analysis of the stem region (SR) and differentiated regions (DR) of the testis revealed 4554 and 3980 specific-enriched genes, respectively. In addition, comparisons with D. melanogaster testis transcriptome revealed homologs of 1204 SR and 389 DR specific-enriched genes that were similarly expressed in equivalent regions of Drosophila testis. Moreover, gene ontology (GO) enrichment analysis was performed for SR-specific enriched genes and DR-specific enriched genes, and the GO terms of several biological processes were enriched, confirming previous findings. This study advances our understanding of spermatogenesis in B. mori and provides an important basis for future research, filling a knowledge gap between fly and mammalian studies.

show abstract

“…Aubergine is part of the argonaut family in Drosophila and is involved in gene silencing in the germline 36 indicating a potential involvement in MSCI starting in spermatocyte cells. Additionally, the RacGAP1/tumbleweed gene (AGAP008912), expressed in the ring canals of premeiotic germ cells to permit cytoplasmic sharing 37 , 38 , showed enrichment in the GSCs (Log2FC: 2.83, p = 0.005), Primary spermatogonia (Log2FC: 2.61, p = 1.12E-15) and Late spermatogonia/Primary spermatocyte (Log2FC: 1.90, p = 1.97E-26) cell clusters, confirming their premeiotic cell state.…”

Section: Resultsmentioning

confidence: 99%

Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome

et al. 2023

View full text Add to dashboard Cite

Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using regulatory sequences to drive transgene expression for applications such as gene drive. However, only very few germline-specific regulatory elements have been characterised with the majority showing leaky expression. This has been shown to considerably reduce the efficiency of current genetic control strategies, which rely on regulatory elements with more tightly restricted spatial and/or temporal expression. Meiotic silencing of the sex chromosomes limits the flexibility of transgene expression to develop effective sex-linked genetic control strategies. Here, we build on our previous study, dissecting gametogenesis into four distinct cell populations, using single-cell RNA sequencing to define eight distinct cell clusters and associated germline cell–types using available marker genes. We reveal overexpression of X-linked genes in a distinct cluster of pre-meiotic cells and document the onset of meiotic silencing of the X chromosome in a subcluster of cells in the latter stages of spermatogenesis. This study provides a comprehensive dataset, characterising the expression of distinct cell types through spermatogenesis and widening the toolkit for genetic control of malaria mosquitoes.

show abstract

Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes

Cited by 5 publications

References 91 publications

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori

Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome

Contact Info

Product

Resources

About