Comparative Transcriptome Analysis between Cytoplasmic Male-sterile Line and Its Maintainer During the Floral Bud Development of Pepper

Lv, Junheng; Liu, Zhoubin; Liu, Yuhua; Ou, Lijun; Deng, Minghua; Wang, Jing; Song, Jingshuang; Ma, Yanqing; Chen, Wenchao; Zhang, Zhuqing; Dai, Xiang; Zou, Xuexiao

doi:10.1016/j.hpj.2020.01.004

Cited by 18 publications

(14 citation statements)

References 48 publications

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“…In the S1 stage, only 42 DEGs were identified. While in the S2 and S3 stages, the number of DEGs (691 and 709), respectively, increased rapidly, which was similar to previous results for eggplant anthers [66] and pepper buds [62]. This shows that the change in gene expression is not obviously caused by male sterility in the early stage of the anther development, and that male sterility mainly affects the gene expression level in the S2 and S3 stages.…”

Section: Male Sterility Occurs Alongside Gene Expression Level Changes In the Middle And Late Stages Of Anther Developmentsupporting

confidence: 89%

“…All primers used in the present study were designed using Primer-Blast software (NCBI, Bethesda, MD, USA) and synthesized by Sangon Biotech (Shanghai, China). The pepper β-actin gene [62] was used as an internal control to normalize the expression data. The primer pairs used are listed in Table S1.…”

Section: Quantitative Real-time Pcr and Candidate Genes Amplificationmentioning

confidence: 99%

“…Mitochondria play an important role in energy production. Mitochondrial deficiencies may lead to failure in acquiring sufficient energy for normal pollen development [62]. In some studies, the sterility-related genes in plant mitochondria, such as orf138, orf79, atp4, and atp6 [8], cause abnormal energy supplies and eventually leading CMS.…”

Section: Mitochondrial Genes Involved In Energy Production Are Related To Cmsmentioning

confidence: 99%

“…Peppers have small anthers, especially in their early stages of development, whi makes it difficult to obtain the anther samples separately for RNA extraction across all t developmental stages. Therefore, the pepper floral buds have been used in previous stu ies for transcriptome analysis [62,63]. In wheat and rice, RNA stabilization solutions ha been used to collect anthers and pollen grains, and qualified RNA has been extracted [6 Therefore, the RNA stabilization solution (RNALater) was used to collect the pepper a thers and extract the RNA successfully in the present study.…”

Section: The Anther Was the Best Organ For Studying Male Sterility In Peppermentioning

confidence: 99%

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Comparative Transcriptome Analysis of the Anthers from the Cytoplasmic Male-Sterile Pepper Line HZ1A and Its Maintainer Line HZ1B

et al. 2021

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Cytoplasmic male-sterility (CMS) is important for the utilization of crop heterosis and study of the molecular mechanisms involved in CMS could improve breeding programs. In the present study, anthers of the pepper CMS line HZ1A and its maintainer line HZ1B were collected from stages S1, S2, and S3 for transcriptome sequencing. A total of 47.95 million clean reads were obtained, and the reads were assembled into 31,603 unigenes. We obtained 42 (27 up-regulated and 15 down-regulated), 691 (346 up-regulated and 345 down-regulated), and 709 (281 up-regulated and 428 down-regulated) differentially expressed genes (DEGs) in stages S1, S2, and S3, respectively. Through Gene Ontology (GO) analysis, the DEGs were found to be composed of 46 functional groups. Two GO terms involved in photosynthesis, photosynthesis (GO:0015986) and photosystem I (GO:0009522), may be related to CMS. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, oxidative phosphorylation (ko00190) and phenylpropanoid biosynthesis (ko00940) were significantly enriched in the S1 and S2 stages, respectively. Through the analysis of 104 lipid metabolism-related DEGs, four significantly enriched KEGG pathways may help to regulate male sterility during anther development. The mitochondrial genes orf470 and atp6 were identified as candidate genes of male sterility for the CMS line HZ1A. Overall, the results will provide insights into the molecular mechanisms of pepper CMS.

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Section: Male Sterility Occurs Alongside Gene Expression Level Changes In the Middle And Late Stages Of Anther Developmentsupporting

confidence: 89%

Section: Quantitative Real-time Pcr and Candidate Genes Amplificationmentioning

confidence: 99%

Section: Mitochondrial Genes Involved In Energy Production Are Related To Cmsmentioning

confidence: 99%

Section: The Anther Was the Best Organ For Studying Male Sterility In Peppermentioning

confidence: 99%

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Comparative Transcriptome Analysis of the Anthers from the Cytoplasmic Male-Sterile Pepper Line HZ1A and Its Maintainer Line HZ1B

et al. 2021

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“…In recent years, many studies have used transcriptome sequencing to investigate male sterility, including studies in pepper (Lv et al, 2020), Catalpa bungei (Mao et al, 2017), maize (Xue et al, 2019), andsoybean (Li et al, 2019). However, no study has investigated male sterility of Prunus sibirica using transcriptome sequencing.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis reveals candidate genes for male sterility in Prunus sibirica

Chen

Zhang

et al. 2021

PeerJ

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Background The phenomenon of male sterility widely occurs in Prunus sibirica and has a serious negative impact on yield. We identified the key stage and cause of male sterility and found differentially expressed genes related to male sterility in Prunus sibirica, and we analyzed the expression pattern of these genes. This work aimed to provide valuable reference and theoretical basis for the study of reproductive development and the mechanisms of male sterility in Prunus sibirica. Method The microstructures of male sterile flower buds and male fertile flower buds were observed by paraffin section. Transcriptome sequencing was used to screen genes related to male sterility in Prunus sibirica. Quantitative real-time PCR analysis was performed to verify the transcriptome data. Results Anther development was divided into the sporogenous cell stage, tetrad stage, microspore stage, and pollen maturity stage. Compared with male fertile flower buds, in the microspore stage, the pollen sac wall tissue in the male sterile flower buds showed no signs of degeneration. In the pollen maturity stage, the tapetum and middle layer were not fully degraded, and anther development stopped. Therefore, the microspore stage was the key stage for anther abortion , and the pollen maturity stage was the post stage for anther abortion. A total of 4,108 differentially expressed genes were identified by transcriptome analysis. Among them, 1,899 were up-regulated, and 2,209 were down-regulated in the transcriptome of male sterile flower buds. We found that “protein kinase activity”, “apoptosis process”, “calcium binding”, “cell death”, “cytochrome c oxidase activity”, “aspartate peptidase activity”, “cysteine peptidase activity” and other biological pathways such as “starch and sucrose metabolism” and “proteasome” were closely related to male sterility in Prunus sibirica. A total of 331 key genes were preliminarily screened. Conclusion The occurrence of male sterility in Prunus sibirica involved many biological processes and metabolic pathways. According to the results of microstructure observations, related physiological indexes determination and transcriptome analysis, we reveal that the occurrence of male sterility in Prunus sibirica may be caused by abnormal metabolic processes such as the release of cytochrome c in the male sterile flower buds, the imbalance of the antioxidant system being destroyed, and the inability of macromolecular substances such as starch to be converted into soluble small molecules at the correct stage of reproductive development, resulting in energy loss. As a result, the tapetum cannot be fully degraded, thereby blocking anther development, which eventually led to the formation of male sterility.

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Potential candidate genes and pathways related to cytoplasmic male sterility in Dianthus spiculifolius as revealed by transcriptome analysis

et al. 2023

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Comparative Transcriptome Analysis between Cytoplasmic Male-sterile Line and Its Maintainer During the Floral Bud Development of Pepper

Cited by 18 publications

References 48 publications

Comparative Transcriptome Analysis of the Anthers from the Cytoplasmic Male-Sterile Pepper Line HZ1A and Its Maintainer Line HZ1B

Comparative Transcriptome Analysis of the Anthers from the Cytoplasmic Male-Sterile Pepper Line HZ1A and Its Maintainer Line HZ1B

Transcriptomic analysis reveals candidate genes for male sterility in Prunus sibirica

Potential candidate genes and pathways related to cytoplasmic male sterility in Dianthus spiculifolius as revealed by transcriptome analysis

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