Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

Abstract

Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.

Genetic approaches to dissect plant nonhost resistance mechanisms

“…With the development of the next‐generation sequencing technologies, candidate genes involved in NHR can be more efficiently identified through (pan)genomics and comparative transcriptomics studies (Gangurde et al, 2021 ; Iven et al, 2012 ; Tufan et al, 2009 ). When such gene expression information is coupled with VIGS and CRISPR, the reverse genetics approach for identification of genes involved in NHR can become more efficient.…”

Genome-Wide Characterization of Ascorbate Peroxidase Gene Family in Peanut (Arachis hypogea L.) Revealed Their Crucial Role in Growth and Multiple Stress Tolerance

“…Cultivated peanut is widely known as an essential oilseed, protein-enrich food crop worldwide and retains important breeding traits during domestication (Zhuang et al, 2019;Bohra et al, 2022). Even so, peanut production is still substantially influenced by numerous biotic and abiotic factors (Agarwal et al, 2018;Gangurde et al, 2020Gangurde et al, , 2021Kumar et al, 2020;Shasidhar et al, 2020;Sinha et al, 2020;Jadhav et al, 2021;Pandey et al, 2021;Soni et al, 2021;Aravind et al, 2022;Bomireddy et al, 2022;Liu et al, 2022;Patel et al, 2022). When plants are exposed to diverse abiotic and biotic factors, APX enzyme as a primary marker can quickly eliminate unnecessary H 2 O 2 (i.e., ROS scavenging) from plant cells by adjusting several physiological and biochemical activities to safeguard cells from the noxiousness of overproduction of ROS (Das and Roychoudhury, 2014;Mittler, 2017;Hasanuzzaman et al, 2020Hasanuzzaman et al, , 2021.…”

“…Cultivated peanut/groundnut (A. hypogaea L.), an allotetraploid crop, is one of the most valuable and economic oilseed food crops globally (Agarwal et al, 2018;Bertioli et al, 2019;Chen X. et al, 2019;Zhuang et al, 2019). This crop is being widely cultivated in the tropical and subtropical regions globally; however, several abiotic and biotic factors significantly affect its growth and production, including many important agronomic traits (Agarwal et al, 2018;Gangurde et al, 2020Gangurde et al, , 2021Kumar et al, 2020;Pandey et al, 2020;Shasidhar et al, 2020;Sinha et al, 2020;Jadhav et al, 2021;Soni et al, 2021;Aravind et al, 2022;Bomireddy et al, 2022;Liu et al, 2022;Patel et al, 2022). Therefore, it is vital to identify new potential genes associated with multiple stress tolerance and trait improvement in peanut for better protein-rich food supply, particularly in Asian and African countries.…”

Genome-wide identification of germin-like proteins in peanut (Arachis hypogea L.) and expression analysis under different abiotic stresses

“…Peanut is now a resource-rich legume crop with massive transcriptome data ( Clevenger et al., 2016 ; Sinha et al., 2020 ; Gangurde et al., 2021 ). Trait dissection and candidate gene discovery with linkage and association mapping attempted for several traits, for instance, yield traits ( Gangurde et al., 2020 ; Pandey et al., 2020 ; Jadhav et al., 2021 ), fresh seed dormancy ( Gangurde et al., 2020 ; Kumar et al., 2020 ; Bomireddy et al., 2022 ), disease resistance ( Dodia et al., 2019 ; Shasidhar et al., 2020 ), and aflatoxin contamination ( Pandey et al., 2019 ; Khan et al., 2020 ; Soni et al., 2020 ).…”