Comparative transcriptome analysis of differentially expressed genes and pathways in Procambarus clarkii (Louisiana crawfish) at different acute temperature stress

Lu, Luo; Huang, Jianhua; Liu, Dongliang; Jiang, Shigui; Zhou, Fa-Lin; Jiang, Song; Yang, Qian; Li, Yundong; Li, Tao; Tan, Li-Qi; Yang, Lishi

doi:10.1016/j.ygeno.2022.110415

Cited by 10 publications

(2 citation statements)

References 58 publications

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“…Te heat shock proteins are classic molecular chaperones or stress proteins distributed ubiquitously in tissues. Tey were proven to protect cellular functions and structures from many types of stress and maintain cellular homeostasis [24][25][26]. HSP90 can prevent the aggregation of heatdenatured proteins and unstable proteins in vitro, usually combined with HSP70 and HSP40 [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Effect of Acute Salinity Stress on Metabolism, Antioxidant Status, and Histological Structure of Procambarus clarkii

Liu²,

Huang

et al. 2023

Aquaculture Research

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Procambarus clarkii is a well-known invasive species with a strong environmental tolerance that has spread worldwide. Crayfish were exposed to five different salinities (0, 6, 12, 18, and 24 parts per thousand (ppt)) to determine how salinity affects physiological and histological responses. The metabolism-related enzymes pyruvate kinase (PK) and fatty acid synthase (FAS) activities of the hepatopancreas and gill tissues significantly decreased with an increase of salinity (6, 12, 18, 24 ppt), except the increase of FAS activity at 6 ppt. Salinity caused an immune disorder, as displayed by the decreased activities of lysozyme (LSZ) and superoxide dismutase (SOD). In contrast, catalase (CAT) activities showed increased activity. The heat shock protein 90 (HSP90) concentration significantly increased at 6 ppt and then significantly decreased at 24 ppt in the hepatopancreas and gill tissues. A HE section revealed that salinity stress influenced the tissue structures. High salinity (24 ppt) damaged the hepatopancreas and deformed the gills. In conclusion, P. clarkii is resistant to acute exposure to low salinities and suffers physiological damage when exposed to hyperosmotic salinities. Our study provides a valuable reference to analyse the adaptation mechanisms of crayfish in response to salinity.

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Section: Discussionmentioning

confidence: 99%

Effect of Acute Salinity Stress on Metabolism, Antioxidant Status, and Histological Structure of Procambarus clarkii

Liu²,

Huang

et al. 2023

Aquaculture Research

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“…The transcriptional plasticity that controls gene expression in organisms is remarkably flexible, constantly reconfiguring itself to respond and adapt to perturbations [13]. Increasing investigations of phenotypic plasticity have revealed that numerous aquatic ectotherms can minimize acute thermal damage through multiple cellular processes, including the involvement of molecular chaperones, cell signaling, immune responses, cytoskeletal organization, energy metabolism, and protein turnover [15][16][17][18]. However, most studies only provided a generalized snapshot of molecular responses by comparing samples between two statuses (normal temperature vs. high temperature) at a single time point.…”

Section: Introductionmentioning

confidence: 99%

Time-Course and Tissue-Specific Molecular Responses to Acute Thermal Stress in Japanese Mantis Shrimp Oratosquilla oratoria

Zhang

Sha

Cheng

2023

IJMS

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Current understanding of adaptability to high temperatures is increasingly important as extreme weather events that can trigger immediate physiological stress in organisms have occurred more frequently. Here, we tracked transcriptomic responses of gills, hepatopancreas, and muscle to acute thermal exposure at 30 °C for 0.5, 6, and 12 h in an economically important crustacean, Oratosquilla oratoria, to gain a preliminary understanding of the tissue-specific and dynamic physiological regulation process under acute heat stress. The unique physiological responses of muscle, hepatopancreas, and gills to acute thermal stress were associated with protein degradation, lipid transport, and energy metabolism in O. oratoria, respectively. Functional enrichment analysis of differentially expressed transcripts and heat-responsive gene clusters revealed a biphasic protective responsiveness of O. oratoria developed from the early responses of signal transduction, immunity, and cytoskeleton reorganization to the responses dominated by protein turnover and energy metabolism at the mid-late stages under acute heat stress. Noteworthy, trend analysis revealed a consistently upregulated expression pattern of high molecular weight heat shock protein (HSP) family members (HSP60, HSP70, and HSP90) during the entire thermal exposure process, highlighting their importance for maintaining heat resistance in O. oratoria. Documenting the whole process of transcriptional responses at fine temporal resolution will contribute to a far-reaching comprehension of plastic responses to acute heat stress in crustaceans, which is critical in the context of a changing climate.

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Transcriptomic analyses provide new insights into immune response of the Procambarus clarkii intestines to Aeromonas hydrophila challenge

Liu,

Bian,

Jiang

et al. 2024

Aquaculture Reports

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Comparative transcriptome analysis of differentially expressed genes and pathways in Procambarus clarkii (Louisiana crawfish) at different acute temperature stress

Cited by 10 publications

References 58 publications

Effect of Acute Salinity Stress on Metabolism, Antioxidant Status, and Histological Structure of Procambarus clarkii

Effect of Acute Salinity Stress on Metabolism, Antioxidant Status, and Histological Structure of Procambarus clarkii

Time-Course and Tissue-Specific Molecular Responses to Acute Thermal Stress in Japanese Mantis Shrimp Oratosquilla oratoria

Transcriptomic analyses provide new insights into immune response of the Procambarus clarkii intestines to Aeromonas hydrophila challenge

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