Comparative transcriptome analysis of molecular mechanism underlying gray-to-red body color formation in red crucian carp (Carassius auratus, red var.)

Zhang, Yongqin; Liu, Jinhui; Peng, Liangyue; Ren, Li; Zhang, Huiqin; Zou, Lijun; Liu, Wenbin; Xiao, Yamei

doi:10.1007/s10695-017-0379-7

Cited by 72 publications

(51 citation statements)

References 46 publications

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“…Also, the lower expression levels of tyr and tyrp‐1 genes under prolonged light treatment, which was reflected by high RGB and pale pink colour of fish, suggest that the prolonged light possibly caused harmful effects on melanin production. Similar results were obtained in Carassius auratus whereby tyrp‐1 gene decreased as the colour changed from grey to red (Zhang et al, ). Moreover, the mutation of tyrp‐1 gene in D. rerio caused the death of some melanophores (Krauss, Geiger‐Rudolph, Koch, Nusslein‐Volhard, & Irion, ).…”

Section: Discussionsupporting

confidence: 80%

“…In this study, we found that artificial photoperiod manipulation affected the contents of tyrosinase and cysteine. Based on colour observation after the experiment, these results indicate that tyrosinase and cysteine determine the melanin content in fish as suggested by Schwahn, Xu, Herrin, Bales, and Medrano, () and Zhang et al (). Generally, the synthesis of pheomelanin is facilitated by cysteine, which induces red/yellow colour (Jiang et al, ; Watson et al, ; Zhu et al, ).…”

Section: Discussionsupporting

confidence: 76%

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Effects of photoperiod on growth performance and melanogenesis pathway for skin pigmentation of Malaysian red tilapia

Atuganile

Zhu

et al. 2020

Aquac Res

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Farming of red tilapia is increasing rapidly. However, its commercial farming development is challenged by lack of clear information on genetic basis for skin colour and pigmentation differences due to environmental changes. This study investigated the effects of photoperiod (light:dark, L:D) on the growth and skin colour variation of Malaysian red tilapia. A total of 180 fish weighing 150.48 ± 0.44 g were reared under natural photoperiod (13L:11D, control), prolonged lightness (24L:0D) and prolonged darkness (0L:24D) in three replicates for 78 days. The weight gain of fish cultured under both prolonged light and darkness were significantly higher than fish under natural photoperiod. The tyrosinase level in ventral skin was significantly higher for fish cultured under prolonged darkness condition than in the other two photoperiod regimes. Contrary, the cysteine level in the dorsal skin was significantly higher in the fish cultured under natural photoperiod than in prolonged light and darkness. The relative mRNA expressions of SRY‐related HMG‐Box 10 (sox 10), tyrosine (tyr), tyrosine‐related protein 1 (tyrp‐1) and solute carrier family 7 member 11 (slc7a11) genes were significantly higher in ventral skin of fish under prolonged darkness than the other two photoperiods. This study demonstrates that photoperiod has an impact on melanogenesis and growth of red tilapia. Understanding the effects of photoperiod on genetic basis of red tilapia will help in selective breeding programme of the important economic traits for the development of commercial red tilapia farming.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 76%

Effects of photoperiod on growth performance and melanogenesis pathway for skin pigmentation of Malaysian red tilapia

Atuganile

Zhu

et al. 2020

Aquac Res

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“…Zhu et al () conducted a transcriptome analylsis of three different coloured varieties red tilapia and found that several key DEGs were related to pigment synthesis, that is, tyr, tyrp1, silv, sox10, slc24a5, cbs and slc7a11. A study of skin transcriptomes for red crucian carp at different developmental stages identified a number of DEGs associated with the melanogenesis, tyrosine metabolism and pteridine metabolism (Zhang, Liu, Peng, et al, ; Zhang, Liu, Fu, et al, ). In another study, to further explore the genetic mechanisms underlying body colour formation in crucian carp, Zhang et al (Zhang, Liu, Peng, et al, ; Zhang, Liu, Fu, et al, ) performed skin transcriptome analysis of red crucian carp and white crucian carp and suggested that genes related to MAPK, Wnt, the cell cycle, endocytosis signalling pathways and variations in melanogenesis might be responsible for the colour variation in this species.…”

Section: Discussionmentioning

confidence: 99%

“…Among these, we mainly focused on the cGMP‐PKG signalling pathway because it could affect pteridine metabolism. The yellow to reddish pteridine pigments of xanthophores are synthesized from guanosine triphosphate (GTP) through the pteridine synthesis pathway, and the GTP (which can be hydrolysed to form cGMP) is essential to signal transduction in second‐messenger mechanisms (Zhang, Liu, Fu, et al, ; Zhang, Liu, Peng, et al, ). Therefore, the DEGs involved in the cGMP‐PKG signalling pathway, including upregulated genes (ADCY2, KCNMB2, PLCB3 and PPP3CA) and downregulated genes (CAC1S, ATP1A2, ATP2A1, ATP2A2, MYLK3, MYLK4, PRKG1 and PRKG2) in Y, could likely become candidate genes for colour variation.…”

Section: Discussionmentioning

confidence: 99%

Comparative skin transcriptome between common carp and the variety Jinbian carp (Cyprinus carpiov. jinbian)

et al. 2019

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Jinbian carp, a variety of common carp (Cyprinus carpio v. jinbian) with black skin colour and two yellow stripes on both sides of its dorsal fin, has become a popular economic fish species as an edible delicacy and for its unique coloration. However, the genetic background of Jinbian carp is not well documented and the molecular mechanism of the yellow or black colour variation of the skin of this fish is not yet clear. Therefore, it is essential to understand the molecular mechanism of colour variation using advanced technology. In this study, using wild and Jinbian common carp as experimental materials, we performed RNA sequencing transcriptomic analysis with regard to the yellow and black skin colour in these fish. As a result, we identified 162 differentially expressed genes (DEGs) between yellow (Y) and black (B) skin derived from the same Jinbian carp and 2,475 DEGs between yellow skin in Jinbian carp (Y) and black skin in wild common carp (W). Gene functional analysis revealed that most of DEGs were involved in pigmentation‐related and metabolic pathways, among which we mainly focused on the cGMP‐PKG signalling pathway and adrenergic signalling in the cardiomyocyte pathway. In addition, our transcriptome results suggested that genes associated with cysteine metabolism, including scavenger receptor cysteine‐rich type 1 protein M130 (CACNA1E), cysteine N‐palmitoyltransferase HHAT‐like protein (HHATL) and soluble scavenger receptor cysteine‐rich domain‐containing protein (SSC5D) could serve as candidate genes for colour variation in Jinbian carp. In conclusion, our results identify candidate genes and potential pathways involved in colour variation and provide useful genetic information for further breeding of Jinbian carp.

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“…The crucian carp Carassius auratus is a member of the family Cyprinidae (Teleostei) [1]. It is one of the important fish species in aquaculture, which is distributed widely in both Europe and Asia [2,3].…”

Section: Introductionmentioning

confidence: 99%

Skin transcriptome analysis of the genes involved in mucous immunity and discovery of SSRs and SNPs in Crucian carp Carassius auratus

wang¹,

Hu²,

Lv³

et al. 2019

Preprint

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Fish skin is first line of defence against attachment and penetration of pathogens. Crucian carp (Carassius auratus) is one of the major freshwater species and important food fish in China, yet its molecular mechanism of skin immune response remains unclear. In this study, a de novo transcriptome assembly of crucian carp skin was performed by using Illumina Hiseq 2000 platform. A total of 49,154,776 unigenes were assembled, among which 60,824 (25.16%) unigenes were annotated against the NCBI database. Gene ontology and KEGG mapping assigned the terms to 37,103 (39.15%), 9,337 (23.23%) unigenes, repectively. The best represented KEGG categories were immune system (8,871, 20.50%) and signal transduction (7,805, 18.04%), and several mucin proteins (MUC1, MUC2, MUC5B, MUC5AC, MUC18, etc.) as the main membrane-bound and secreted components of the mucosal layer, implying the differentiation of immune responses in the crucian carp skin. Moreover, the results revealed that potential 28,928 SSRs and 249,964 SNPs were detected in crucian carp. This is the first report on transcriptome analysis in the skin of C. auratus, which contribute to understanding the molecular mechanisms of mucous immunogenectics response and epidermal mucus secretion of the skin in fish.

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Comparative transcriptome analysis of molecular mechanism underlying gray-to-red body color formation in red crucian carp (Carassius auratus, red var.)

Cited by 72 publications

References 46 publications

Effects of photoperiod on growth performance and melanogenesis pathway for skin pigmentation of Malaysian red tilapia

Effects of photoperiod on growth performance and melanogenesis pathway for skin pigmentation of Malaysian red tilapia

Comparative skin transcriptome between common carp and the variety Jinbian carp (Cyprinus carpiov. jinbian)

Skin transcriptome analysis of the genes involved in mucous immunity and discovery of SSRs and SNPs in Crucian carp Carassius auratus

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