Comparative transcriptome analysis of pheromone biosynthesis-related gene expressions in Plutella xylostella (L.)

Hyun, Wook; Jung, Chung Ryul; Hwang, Yoon-Jeong; Lee, Dae‐Weon

doi:10.1016/j.aspen.2017.09.008

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…To explore the molecular mechanism of α‐linolenic acid synthesis in G. pyloalis pupae, we constructed the pupal transcriptome dataset for G. pyloalis and identified two GpylFASs and five GpylDesats from it. The number of FAS genes identified in G. pyloalis was similar to that present in other insects, such as P. xylostella (four FAS genes), 31 Colaphellus bowringi (two FAS genes) 32 and Agrotis ipsilon (one FAS gene) 33 . On the contrary, the number of Desat genes in G. pyloalis was quite different from other insect species, such as S. litura (18 Desat genes), 30 Maruca vitrata (20 Desat genes) 34 and Agrotis segetum (10 Desat genes) 35 .…”

Section: Discussionsupporting

confidence: 67%

Fatty acid synthases and desaturases are essential for the biosynthesis of α‐linolenic acid and metamorphosis in a major mulberry pest, Glyphodes pyloalis walker (Lepidoptera: Pyralidae)

Wang

Song

Hwarari

et al. 2022

Pest Management Science

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BACKGROUND: ⊍-linolenic acid is an essential unsaturated fatty acid in organisms. However, there is a large gap between ⊍-linolenic acid accumulation and its synthesis mechanism in insects. Fatty acid synthases (FASs) and desaturases (Desats) are vital enzymes required for the synthesis of unsaturated fatty acids.RESULTS: The pupae of Glyphodes pyloalis (Lepidoptera: Pyralidae), which is a destructive pest of mulberry trees, contain the highest level of ⊍-linolenic acid compared to other life-history stages. To further explore the synthesis mechanism of ⊍-linolenic acid in G. pyloalis pupae, we constructed a pupal transcriptome dataset and identified 106 genes related to fatty acid metabolism from it. Among these, two fatty acid synthases (GpylFAS) and five desaturases (GpylDesat) were identified. A qRT-PCR validation revealed that GpylFAS1 and GpylDesat1, 2, 3, 5 were expressed highest at pupal stages. Furthermore, the content of ⊍-linolenic acid decreased significantly after silencing GpylFAS1 and GpylDesat5, respectively. Besides, knocking down Gpyl-FAS1 or GpylDesat5 resulted in more malformed pupae and adults, as well as lower emergence rates. Meanwhile, silencing Gpyl-FAS1 or GpylDesat5 affected the expressions of the other GpylFASs and GpylDesats.CONCLUSION: The present results illustrate the pivotal function of FASs and Desats in ⊍-linolenic acid biosynthesis and metamorphosis in insects. Our research also broadens the sources of unsaturated fatty acids, especially for ⊍-linolenic acid from insects, and provides novel insights for the management of mulberry insect pests from the perspective of utilization rather than control.

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Section: Discussionsupporting

confidence: 67%

Fatty acid synthases and desaturases are essential for the biosynthesis of α‐linolenic acid and metamorphosis in a major mulberry pest, Glyphodes pyloalis walker (Lepidoptera: Pyralidae)

Wang

Song

Hwarari

et al. 2022

Pest Management Science

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“…The desaturases are expressed at the highest level whereas the next enzyme in the pathway, the fatty-acid reductase is expressed at a lower level. This phenomenon has also been found in transcriptome studies of other moth pheromone glands [25–30, 34–38]. The next enzymes in the pathway are either an alcohol dehydrogenase in those moths that produce aldehydes or an acetyltransferase in those moths that produce acetate esters; neither of which has been identified at the molecular level.…”

Section: Discussionsupporting

confidence: 53%

Pheromone gland transcriptome of the pink bollworm moth, Pectinophora gossypiella: Comparison between a laboratory and field population

et al. 2019

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The pink bollworm, Pectinophora gossypiella , is a world-wide pest of cotton and in some parts of the cotton growing region is controlled by the mating disruption technique using synthetic sex pheromone. The sex pheromone consists of two compounds, ( Z , Z )- and ( Z , E )-7,11-hexadecadienyl acetates, in about a 50:50 ratio. However, recently, a population with sex pheromone compound ratios of about 62:38 were found in cotton fields that use mating disruption in Israel. To investigate how the change developed, we compared the pheromone gland transcriptomes between a reference laboratory population and a population obtained from an Israeli cotton field utilizing mating disruption. We analyzed four biological replicates from each population and found transcripts encoding 17 desaturases, 8 reductases, and 17 candidate acetyltransferases in both populations, which could be involved in sex pheromone biosynthesis. The expression abundance of some genes between the two populations was different. Some desaturases and candidate acetyltransferases were found to have mutated in one of the populations. The differentially expressed genes play potential roles in sex pheromone biosynthesis and could be involved in causing altered female sex pheromone ratios in the field population.

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“…Pheromones play an important role in mating by promoting mate attraction and selection, alertness, defences and aggregation [48][49][50]. Carboxylases are involved in the production and biosynthesis of pheromones [51,52], therefore, the upregulation of carboxylases (ACC-2) in mated females within 7 days may attract males to increase the success rate of mating.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females

Huo

Liu

Yang

et al. 2020

Insects

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Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up- and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

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Comparative transcriptome analysis of pheromone biosynthesis-related gene expressions in Plutella xylostella (L.)

Cited by 9 publications

References 35 publications

Fatty acid synthases and desaturases are essential for the biosynthesis of α‐linolenic acid and metamorphosis in a major mulberry pest, Glyphodes pyloalis walker (Lepidoptera: Pyralidae)

Fatty acid synthases and desaturases are essential for the biosynthesis of α‐linolenic acid and metamorphosis in a major mulberry pest, Glyphodes pyloalis walker (Lepidoptera: Pyralidae)

Pheromone gland transcriptome of the pink bollworm moth, Pectinophora gossypiella: Comparison between a laboratory and field population

Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females

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