Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Abstract

Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.

Partial replacement of seawater with crude salt in giant freshwater prawn Macrobrachium rosenbergii hatchery operation

“…Our result is completely in agreement with Shuhaimi‐Othman et al (2011) in terms of the relationship between Cu concentration with survival and production, as Cu is a functional part of respiratory protein hemocyanin. High concentrations of Zn and Pb act as osmoregulatory inhibitors in freshwater prawns PL, the lack of substantial influence of Zn and Pb suggests crude salt as a suitable option for larvae rearing (Liu et al, 2021). Cd is a non‐essential and toxic heavy metal for both human and aquatic organisms, but our result demonstrates a significant relationship with the rearing cycle, survival rate and production, and so it deserves further deep assessment (Idrus et al, 2021).…”

Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress

“…The hepatopancreas is a vital metabolic and digestive organ of crustaceans (Wang et al 2022). The changes in hepatopancreatic structure are signi cantly related to discrepancies in physiological status (Liu et al 2021). As the breathing organ of crustaceans, the gills are in direct contact with the external environment (Bechmann et al 2019).…”

Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

“…By using next‐generation sequencing technologies combined with advanced bioinformatic tools, researchers are able to analyze transcriptome‐wide responses to environmental stressors in both model and non‐model species (Feldmeyer et al, 2011; Lenz et al, 2014; Richardson & Sherman, 2015; Riesgo et al, 2012). The transcriptomic literature investigating decapod stressors has increased in recent years, from response to pathogens (He et al, 2021; Jin & Zhu, 2019), hypoxia (Sun et al, 2014, 2020), aquaculture waste products (Liu et al, 2020; Yu et al, 2019), and heavy metals (Liu et al, 2021; Zhang et al, 2019), to adaptation to extreme deep‐sea environments (J. Cheng, Hui, et al, 2019; Hui et al, 2018). Following the Deepwater Horizon oil spill in the Gulf of Mexico in 2010, several studies used transcriptomic profiling in field and laboratory settings to assess the impacts of crude oil exposure on native marine fish species (Pilcher et al, 2014; Whitehead et al, 2012).…”