Comparative transcriptome analysis of the Eureka lemon in response to Citrus yellow vein virus infection at different temperatures

Wang, Ying; Ping, Liao; Fa, Zhao Jin; Kai, Zhang Xing; Liu, Chuang; An, Xiao Ping; Yong, Zhou Chang; Zhou, Yan

doi:10.1016/j.pmpp.2022.101832

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…Shigyoku [64]. Wang et al found that MYBs3 was involved in regulating resistance-related pathways in Eureka lemon, including phenylpropanoid, flavone/flavonol, and isoflavonoid pathways [65].…”

Section: Transcription Factors Involved In Pigment Accumulationmentioning

confidence: 99%

The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Tea (Camellia sinensis) Pericarp

Du,

Lin,

Zhang

et al. 2023

IJMS

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To elucidate the molecular mechanisms underlying the differential metabolism of albino (white), green, and purple pericarp coloration, biochemical profiling and transcriptome sequencing analyses were performed on three different tea pericarps, Zhongbaiyihao (Camellia sinensis L. var. Zhongbai), Jinxuan (Camellia sinensis L. var. Jinxuan), and Baitangziya (Camellia sinensis L. var. Baitang). Results of biochemical analysis revealed that low chlorophyll content and low chlorophyll/carotene ratio may be the biochemical basis for albino characteristics in the ‘Zhongbaiyihao’ pericarp. The differentially expressed genes (DEGs) involved in anthocyanin biosynthesis, including DFR, F3′5′H, CCoAOMT, and 4-coumaroyl-CoA, were highly expressed in the purple ‘Baitangziya’ pericarp. In the chlorophyll synthesis of white pericarp, GUN5 (Genome Uncoupled 5) and 8-vinyl-reductase both showed high expression levels compared to the green one, which indicated that albino ‘Zhongbaiyihao’ pericarp had a higher chlorophyll synthesis capacity than ‘Jinxuan’. Meanwhile, chlorophyllase (CLH, CSS0004684) was lower in ‘Baitang’ than in ‘Jinxuan’ and ‘Zhongbaiyihao’ pericarp. Among the differentially expressed transcription factors, MYB59, WRKY41-like2 (CS ng17509), bHLH62 like1 (CS ng6804), and bHLH62-like3 (CSS0039948) were downregulated in Jinxuan pericarp, suggesting that transcription factors played a role in regulating tea pericarp coloration. These findings provide a better understanding of the molecular mechanisms and theoretical basis for utilizing functional components of tea pericarp.

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Section: Transcription Factors Involved In Pigment Accumulationmentioning

confidence: 99%

The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Tea (Camellia sinensis) Pericarp

Du,

Lin,

Zhang

et al. 2023

IJMS

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“…MYB, one of the most prominent transcription factor families in plants, regulates a variety of biological processes, including specific development, metabolism, response to abiotic stresses [ 34 , 35 ] in Arabidopsis thaliana [ 36 ], Zea mays [ 37 ], and G. max [ 38 ]. MYB1R1 is involved in gene regulation under stress in plants [ 39 , 40 ]. To validate the best selected reference genes, AeMYB1R1 expression levels under different conditions were investigated using the most stable and least stable reference genes or combinations.…”

Section: Introductionmentioning

confidence: 99%

Selection and Validation of Reference Genes in Different Tissues of Okra (Abelmoschus esculentus L.) under Different Abiotic Stresses

Zhu

Jian-xiang

Tang

et al. 2023

Genes

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Okra (Abelmoschus esculentus L.) is a particular vegetable with both edible and medicinal values. However, the expression pattern of the okra reference genes in response to abiotic stress has not been explored. In the present study, 18 potential reference genes were selected from okra in various tissues and abiotic stress conditions, and their expression levels were detected by Real-Time quantitative PCR (RT-qPCR). Their expression stabilities were calculated by four algorithms (geNorm, NormFinder, BestKeeper, and RefFinder). Under cold stress, the most stable genes included GAPC1 and CYP (leaf), CYP and ACT7 (root), HIS6 and GAPC1 (stem), and HIS6 and 60s (different tissues). Under salt stress, EF-1α and UBQ (leaf), EF-1α and UBQ (root), TUA4 and Eif (stem), and HIS6 and Eif (different tissues) were the most stable genes. Under drought stress, UBQ and Eif in the leaf, HIS6 and Eif in the root, TUA4 and HIS6 in the stem, and UBQ and Eif in different tissues were most stably expressed in okra. In addition, complete sequencing results by RefFinder showed that HIS6 and ACT7 in the leaf, HIS6 and Eif in the root, UBC5B and 60s in the stem, and HIS6 and Eif in different tissues, were most the suitable reference genes for okra. Furthermore, AeMYB1R1 transcription factor was used to verify the reliability of RT-qPCR values. In summary, this study was carried out to demonstrate the potential reference genes of okra under abiotic stress, aiming to provide a molecular basis for functional gene analysis and regulatory mechanism research of okra.

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Managing virus diseases in citrus: Leveraging high-throughput sequencing for versatile applications

Chen,

Xuan,

Yang

et al. 2024

Horticultural Plant Journal

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Comparative transcriptome analysis of the Eureka lemon in response to Citrus yellow vein virus infection at different temperatures

Cited by 6 publications

References 36 publications

The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Tea (Camellia sinensis) Pericarp

The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Tea (Camellia sinensis) Pericarp

Selection and Validation of Reference Genes in Different Tissues of Okra (Abelmoschus esculentus L.) under Different Abiotic Stresses

Managing virus diseases in citrus: Leveraging high-throughput sequencing for versatile applications

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