Comparative transcriptome analysis provides insight into regulation pathways and temporal and spatial expression characteristics of grapevine (Vitis vinifera) dormant buds in different nodes

Abstract

Background: Bud dormancy is a strategic mechanism plants developed as an adaptation to unfavorable environments. The grapevine (Vitis vinifera) is one of the most ancient fruit vine species and vines are planted all over the world due to their great economic benefits. To better understand the molecular mechanisms underlying bud dormancy between adjacent months, the transcriptomes of 'Rosario Bianco' grape buds of 6 months and three nodes were analyzed using RNA-sequencing technology and pair-wise comparison. From November to April of the following year, pairwise comparisons were conducted between adjacent months. Results: A total of 11,647 differentially expressed genes (DEGs) were obtained from five comparisons. According to the results of cluster analysis of the DEG profiles and the climatic status of the sampling period, the 6 months were divided into three key processes (November to January, January to March, and March to April). Pair-wise comparisons of DEG profiles of adjacent months and three main dormancy processes showed that the whole grapevine bud dormancy period was mainly regulated by the antioxidant system, secondary metabolism, cell cycle and division, cell wall metabolism, and carbohydrates metabolism. Additionally, several DEGs, such as VvGA2OX6 and VvSS3, showed temporally and spatially differential expression patterns, which normalized to a similar trend during or before April. Conclusion: Considering these results, the molecular mechanisms underlying bud dormancy in the grapevine can be hypothesized, which lays the foundation for further research.

Comparative Transcriptomic Analysis Provides Insight into the Key Regulatory Pathways and Differentially Expressed Genes in Blueberry Flower Bud Endo- and Ecodormancy Release

“…The expression levels of the gene were consistent with the changes in JA content. It retained low expression levels during the process of dormancy release and was upregulated from ecodormancy to bloom; these results are similar to those of previous research [42]. It has been speculated that JA may play an important role in the process of dormancy release.…”

“…Although the transcriptomes of flower buds in endo-and ecodormancy have been widely reported in other species [40][41][42], few studies have been conducted on the process of floral bud break. Recently, in tea [22] and grape [42], transcriptome studies were performed on different states of flower buds from dormancy to bloom, covering paradormancy, endodormancy, ecodormancy, and bud break with flower buds sampled by month, reflecting the gene expression of different states of buds. Due to the lack of detailed sampling at key dormancy breaking times, the detailed process of gene expression regulation by temperature during bud dormancy release cannot be determined.…”

Transcriptomic and metabolomic integration as a resource in grapevine to study fruit metabolite quality traits

“…Subsequent studies also focused on berry development, ripening, and post-harvest of different cultivars ( Pilati et al., 2007 ; Lijavetzky et al., 2012 ; Sweetman et al., 2012 ; Cramer et al., 2014 ; Gouthu et al., 2014 ; Pilati et al., 2014 ; Guo et al., 2016 ; Zenoni et al., 2016 ; Ghan et al., 2017 ; Massonnet et al., 2017 ; Shangguan et al., 2017 ; Balic et al., 2018 ; Fasoli et al., 2018 ; Savoi et al., 2019 ; Cramer et al., 2020 ; Guo et al., 2020 ; Savoi et al., 2021b ; Theine et al., 2021 ). At the same time, other studies considered the development of tendrils and inflorescences ( Díaz-Riquelme et al., 2014 ), buds ( Díaz-Riquelme et al., 2012 ; Pucker et al., 2020 ; Shangguan et al., 2020 ), flower ( Sreekantan et al., 2010 ; Grimplet et al., 2017 ; Vannozzi et al., 2021 ), leaf ( Pervaiz et al., 2016 ), fruits of seeded/seedless cultivars ( Nwafor et al., 2014 ; Royo et al., 2016 ) and roots from V. vinifera and Vitis rootstocks ( Cookson et al., 2013 ; Corso et al., 2015 ; Cochetel et al., 2017 ; Livigni et al., 2019 ). Further studies investigated the plant responses to ozonated water applications ( Campayo et al., 2021 ), the circadian cycle ( Carbonell-Bejerano et al., 2014b ; Rienth et al., 2014a ), the interaction with abiotic stresses such as temperature ( Liu et al., 2012 ; Carbonell-Bejerano et al., 2013 ; Xin et al., 2013 ; Rienth et al., 2014b ; Rienth et al., 2016 ), light ( Pontin et al., 2010 ; Carbonell-Bejerano et al., 2014a ) and water availability ( Perrone et al., 2012 ; Dal Santo et al., 2016b ).…”

Evolution of Aroma Profiles and Potential in Shine Muscat Grape Berry during Ripening

“…The global production of grapes for 2019/2020 was estimated at 23.4 million tons (USDA Foreign Agricultural Service, 2020). According to their utilisation, grape fruits are divided into table, wine and raisin grapes (Shangguan et al, 2020). In Asia, grape cultivation is based mainly on table grapes, and seedless and aromatic grapes are currently favoured by consumers.…”