Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Zhang, Kai; Wu, Zhengdan; Tang, Daobin; Luo, Kai; Lu, Huixiang; Liu, Yingying; Dong, Jie; Wang, Xin; Lv, Changwen; Wang, Jichun; Lü, Kun

doi:10.3389/fpls.2017.00914

Cited by 61 publications

(56 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Elsewhere, the close association of carbohydrate metabolism with storage root development was also revealed on Lycoris sprengeri [20], sweet potato (Ipomoea batatas.) [32], and Sagittaria sagittifolia [33]. Altogether, these ndings con rmed the important role of starch and sucrose metabolism in bulb formation and development of bulbous plants via transcriptome pro ling method.…”

Section: Discussionsupporting

confidence: 59%

See 1 more Smart Citation

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Su-Qin

Yan

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Starch and sucrose metabolism plays a crucial role in the formation and development of bulbs in bulbous plants, which, however, remains unclear and unexplored in the corms of Freesia hybrida, one kind of famous bulbous flower. Herein, we investigated the dynamics of the major form of carbohydrates and related enzyme activities and profiled the transcriptome of freesia corms at four developmental stages with the aim to reveal the relation of starch and sucrose metabolism to corm development and the transcriptional regulation of this metabolic process.Results The content of starch, sucrose and soluble sugars followed an overall upward trend across the corm developmental stages. Activities of the adenosine diphosphoglucose pyrophosphorylase, starch branching enzyme and β-amylase generally followed the pattern of the starch and sucrose level. Activities of sucrose phosphate synthase increased from corm formation till the initial swelling stage and subsequently reached a plateau. Activities of invertase and sucrose synthase peaked at the later rapid swelling stage. Transcriptome analysis revealed a total of 100,999 unigenes, out of which 44,405 unigenes were annotated. Analysis based on Clusters of Orthologous Groups suggested that carbohydrate transport and metabolism (9.34% of the sequences) was prominent across the corm developmental process. Totally 3427 differentially expressed genes were identified and the enrichment analysis directed starch and sucrose metabolism a critical pathway in corm development especially at the rapid swelling stage. Genes encoding key carbohydrate-metabolizing enzymes were identified and their differential expression related to corm development was explored.Conclusions The comparative transcriptome analysis discloses essential roles of starch and sucrose metabolism and the genetic mechanism related to the corm development. The results construct a valuable resource pool for further molecular-level studies, which are helpful for metabolic regulation of carbohydrates and improvement in molecular breeding of Freesia hybrida.

show abstract

Section: Discussionsupporting

confidence: 59%

“…Additionally, Zhang et al [32] found that AGPase, soluble starch synthase, granule-bound starch synthase and SuSy encoding genes mostly peaked in sweet potatoes at 95 d after planting when starch accumulation peaked. Apparently, the genes mainly responsible for starch accumulation varies with plant type.…”

Section: Gene Expression During Corm Developmentmentioning

confidence: 99%

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Su-Qin

Yan

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…CL3807.Contig32_All (Fig 4j). Several enzymes including granule-bound starch synthase (GBSS), starch branching enzyme (SBE) and UDP-glucose pyrophosphorylase (UDPGP), are pivotal in sweet potato starch biosynthesis [60][61][62]. Unigene35652_All and Unigene49607_All related to GBSS were significantly upregulated in the MTRs and LTRs stages of tuberous root development in our work ( Fig.…”

Section: Down-regulated Of Lignin Biosynthesis and Up-regulated Of Camentioning

confidence: 62%

Comparative transcriptome analysis of roots at different development stages reveals the mechanism of root expansion of Callerya speciosa Champ.

Yao

Lan

Huang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Callerya speciosa Champ., a perennial shrub medicinal plant of Fabaceae, is one of the most commonly used in traditional Chinese medicine and food that has been widely cultivated in South China. The tuberous roots of C. speciosa transferred from fibrous roots but few fibrous roots can transfer into tuberous roots. To date, the underlying mechanisms for tuberous root formation and development of C. speciosa have not yet been elucidated.

show abstract

“…Invertase genes also showed differential expression between non-infected and infected plants. A cell wall invertase unigene was upregulated in infected plants, but three vacuolar invertase genes, including the previously reported sweet potato vacuolar invertase genes Ibβfruct2 and Ibβfruct3 [19,20], were downregulated. Furthermore, genes for a plastid glucose transporter (GT) showed downregulated expression in the infected plants, and several other sugar transporter genes were DEGs.…”

Section: Spvd Affects the Expression Levels Of Genes Involved In Carbmentioning

confidence: 75%

“…Based on the RPKM values observed, genes differentially expressed between S14 and H14 were screened by comparing the two libraries based on the combined criteria of q-value (false discovery rate (FDR)) of <0.01 and absolute fold change of RPKM > 2. The differential expression of target genes was validated using the qRT-PCR method described previously [20].…”

Section: Unigene Expression Analysis and Differentially Expressed Genmentioning

confidence: 99%

The Spread and Transmission of Sweet Potato Virus Disease (SPVD) and Its Effect on the Gene Expression Profile in Sweet Potato

Zhang

Wan

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

Sweet potato virus disease (SPVD) is the most devastating viral disease in sweet potato (Ipomoea batatas (L.) Lam.), causing substantial yield losses worldwide. We conducted a systemic investigation on the spread, transmission, and pathogenesis of SPVD. Field experiments conducted over two years on ten sweet potato varieties showed that SPVD symptoms first occurred in newly developed top leaves, and spread from adjacent to distant plants in the field. The SPVD incidence was mainly (but not only) determined by the resistance of the varieties planted, and each variety exhibited a characteristic subset of SPVD symptoms. SPVD was not robustly transmitted through friction inoculation, but friction of the main stem might contribute to a higher SPVD incidence rate compared to friction of the leaf and branch tissues. Furthermore, our results suggested that SPVD might be latent in the storage root. Therefore, using virus-free storage roots and cuttings, purposeful monitoring for SPVD according to variety-specific symptoms, and swiftly removing infected plants (especially during the later growth stages) would help control and prevent SPVD during sweet potato production. Comparative transcriptome analysis revealed that numerous genes involved in photosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, and carotenoid biosynthesis were downregulated following SPVD, whereas those involved in monolignol biosynthesis, zeatin biosynthesis, trehalose metabolism, and linoleic acid metabolism were upregulated. Notably, critical genes involved in pathogenesis and plant defense were significantly induced or suppressed following SPVD. These data provide insights into the molecular changes of sweet potato in response to SPVD and elucidate potential SPVD pathogenesis and defense mechanisms in sweet potato. Our study provides important information that can be used to tailor sustainable SPVD control strategies and guide the molecular breeding of SPVD-resistant sweet potato varieties.

show abstract

Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato

Cited by 61 publications

References 55 publications

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Comparative transcriptome analysis of roots at different development stages reveals the mechanism of root expansion of Callerya speciosa Champ.

The Spread and Transmission of Sweet Potato Virus Disease (SPVD) and Its Effect on the Gene Expression Profile in Sweet Potato

Contact Info

Product

Resources

About