Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus)

Li, Shifan; Yang, Yuhang; Wen, Jiaqi; He, Min; Hu, Qiongbo; Zhang, Ke; Weng, Qunfang

doi:10.1016/j.ecoenv.2023.115890

Cited by 1 publication

(1 citation statement)

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“…To further investigate these effects, proteomics technology and bioinformatics analyses were used to explore alterations in the gene expression levels within the male testes of Plutella xylostella following exposure to 60 Co-γ radiation [33]. In the present study, nine significantly differentially expressed genes in the testes of Plutella xylostella after irradiation treatment were verified, including nuclear factor NF-kappa-B (NFkB), cyclin-dependent-like kinase-5 (CDK5), 3-phosphoinositide-dependent protein kinase-1 (PDK1), inhibitor of nuclear factor kappa-B kinase subunit alpha (IKKα), phosphatidylinositol 4, 5bisphosphate 3-kinase (PI3K), serine/threonine-protein kinase mTOR-like (mTOR), phosphatase and tensin homolog (PTEN), RAC serine/threonine-protein kinase (AKT) and tyrosine-protein kinase hopscotch (JAK2) [34]. We employed double-stranded RNA (dsRNA) injections as a gene knockdown approach to confirm that irradiation reduces the reproductive ability of Plutella xylostella.…”

Section: Introductionmentioning

confidence: 99%

Verification of AKT and CDK5 Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in Plutella xylostella (Linnaeus)

Wen,

Wang,

Zeng

et al. 2024

IJMS

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Plutella xylostella (Linnaeus) mainly damages cruciferous crops and causes huge economic losses. Presently, chemical pesticides dominate its control, but prolonged use has led to the development of high resistance. In contrast, the sterile insect technique provides a preventive and control method to avoid the development of resistance. We discovered two genes related to the reproduction of Plutella xylostella and investigated the efficacy of combining irradiation with RNA interference for pest management. The results demonstrate that after injecting PxAKT and PxCDK5, there was a significant decrease of 28.06% and 25.64% in egg production, and a decrease of 19.09% and 15.35% in the hatching rate compared to the control. The ratio of eupyrene sperm bundles to apyrene sperm bundles also decreased. PxAKT and PxCDK5 were identified as pivotal genes influencing male reproductive processes. We established a dose-response relationship for irradiation (0–200 Gy and 200–400 Gy) and derived the irradiation dose equivalent to RNA interference targeting PxAKT and PxCDK5. Combining RNA interference with low-dose irradiation achieved a sub-sterile effect on Plutella xylostella, surpassing either irradiation or RNA interference alone. This study enhances our understanding of the genes associated with the reproduction of Plutella xylostella and proposes a novel approach for pest management by combining irradiation and RNA interference.

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Section: Introductionmentioning

confidence: 99%