Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Jiang, Chunhao; Yao, Xiefeng; Mi, Dandan; Li, Zi-Jie; Yang, Bingye; Zheng, Ying; Qi, Yongwen; Guo, Jianhua

doi:10.3389/fmicb.2019.00652

Cited by 86 publications

(58 citation statements)

References 46 publications

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“…B. amyloliquefaciens L3 can control Fon by producing diffusible and volatile organic compounds (Wu et al ., 2019). B. velezensis F21 can induce systemic resistance to Fon in watermelon through related genes and phytohormone signalling factors (Jiang et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01

Wang

Liu

et al. 2020

Microbial Biotechnology

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Myriocin, which is produced by Bacillus amyloliquefaciens LZN01, can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon). In the present study, the antifungal mechanism of myriocin against Fon was investigated with a focus on the effects of myriocin on the cell membrane. Myriocin decreased the membrane fluidity and destroyed the membrane integrity of Fon. Significant microscopic morphological changes, including conidial shrinkage, the appearance of larger vacuoles and inhomogeneity of electron density, were observed in myriocin-treated cells. A membrane-targeted mechanism of action was also supported by transcriptomic and proteomic analyses; a total of 560 common differentially expressed genes (DEGs) and 285 common differentially expressed proteins (DEPs) were identified. The DEGs were further verified by using RT-qPCR. The combined analysis between the transcriptome and proteome revealed that the expression of some membrane-related genes and proteins, mainly those related to sphingolipid metabolism, glycerophospholipid metabolism, steroid biosynthesis, ABC transporters and protein processing in the endoplasmic reticulum, was disordered. Myriocin affected the serine palmitoyl transferase (SPT) activity as evidenced through molecular docking. Our results indicate that myriocin has significant antifungal activity owing to its ability to induce membrane damage in Fon.

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Section: Introductionmentioning

confidence: 99%

Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01

Wang

Liu

et al. 2020

Microbial Biotechnology

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“…(dominant microbes). The relative abundance of Trichoderma and Bacillus that have been widely used as a biological control agent against various pathogens were no difference between control samples and S-FOM samples [15,16,17,18]. But, the relative abundance of T. asperellum, T. atroviride, B. anthracis, B. kochii, and so on showed an increased tendency in response to the invasion of F. oxysporum.…”

Section: Discussionmentioning

confidence: 89%

“…and Bacillus spp. [15,16,17,18]. Several studies of biocontrol agents have been reported combination treatment methods using two or more of these agents are more effective in managing the disease than treatment using a single biocontrol agent.…”

Section: Introductionmentioning

confidence: 99%

Responses of Soil Microbial Communities to the Invasion of Fusarium Oxysporum and Screening for Fusarium Antagonistic Microorganisms From Pathogenic Soil

Tian¹,

Gao²

2021

Preprint

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BackgroundFusarium oxysporum is known as a biological pollutant in the farmland soil, that causes vascular wilt on a wide range of cash crops, which is the most destructive disease of plant worldwide. F. oxysporum is a fungus that can colonize and grow saprophytically in plant debris and increase pathogen viability in soil. It is almost impossible to remove once successful colonization. How to effectively reduce the pathogen population and restore the beneficial microbial community in soil is an important subject to control the Fusarium wilt. The main objective of this study is to explore influences of F. oxysporum invasion on the microbial community and to screen efficient biocontrol agents from microorganisms with changing relative abundance.ResultsIn this study, we evaluated that responses of the soil microbial communities to invasion of F. oxysporum. We found that F. oxysporum invasion could modify soil physical-chemical properties, soil enzymes activities, microbial community structure and diversity of the soil microbes. We screened and identified an efficient biocontrol strain, Talaromyces purpurogenus Q2, which could be used for defense and suppression of F. oxysporum in bitter gourd and efficiently decreasing incidence of the Fusarium wilt of bitter gourd in the greenhouse and in the field. The research results of biocontrol mechanisms showed that T. purpureogenus strain Q2 involved in the resistance of soil microbial communities to invasion of F. oxysporum, and the density of F. oxysporum was inhibited by exogenous T. purpureogenus and the resident microbes that strain Q2 promoted their growth. In the co-culture of strain Q2 and F. oxysporum, we further observed that T. purpurogenus Q2 could significantly suppress the mycelia growth and development of F. oxysporum by secreting cell wall degrading enzymes, and hinder cell wall formation by suppressing the gene expression of cell wall biosynthesis, such as FomCFEM that is essential for full virulence and stress tolerance in F. oxysporum.ConclusionTogether we screened a efficient biocontrol agents from microorganisms with resistance to the invasion of F. oxysporum. The mechanisms of Talaromyces purpurogenus strain Q2 protected bitter gourd from F. oxysporum is to reduce the relative abundance of rhizospheric F. oxysporum by inhibiting the growth and development of F. oxysporum. This work can provide a new biocontrol agent for biocontrol of Fusarium wilt and soil-born diseases of other horticultural crops.

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“…In a previous study, a resistant cultivar of banana had an inherently higher capacity to stimulate POD production than a susceptible cultivar ( Aguilar et al, 2000 ). Moreover, B. velezensis can trigger basal immunity in plants ( Jiang et al, 2018 ) by increasing the expression of plant defense-related genes and the activities of some defense enzymes, such as catalase (CAT) and POD ( Jiang et al, 2019 ). In this study, banana seedlings treated with HN03 had a higher POD content than those in the treatment without HN03, and the highest POD content was detected in the treatment with HN03 combined with wormcast.…”

Section: Discussionmentioning

confidence: 99%

The Soil Nutrient Environment Determines the Strategy by Which Bacillus velezensis HN03 Suppresses Fusarium wilt in Banana Plants

Ying

et al. 2020

Front. Plant Sci.

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Biological control agents (BCAs) are considered as one of the most important strategies for controlling Fusarium wilt, and bioorganic fertilizer, in particular, has been extensively investigated. However, little is known regarding how a biocontrol microorganism affects the suppression mechanisms when combined with different amendments. In this study, a pot experiment was performed using banana plants to investigate the different mechanisms by which the biocontrol bacterium Bacillus velezensis HN03 (isolated from our laboratory) and amendments suppress Fusarium wilt. The incidence of banana wilt was decreased under HN03 and was reduced further when HN03 was combined with compost, particularly wormcast. In the suppression of Fusarium wilt, HN03 was found to influence the soil environment in various ways. HN03 increased the peroxidase level, which improves plant defense, and was highest when combined with wormcast, being 69 times higher than when combined with cow dung compost. The high accumulation of Mg and P in the “HN03 + wormcast” and Zn and Mn in the “HN03 + cow dung” treatments was negatively correlated with disease incidence. Furthermore, HN03 re-established the microbial community destroyed by the pathogen and further increased the level of suppression in the wormcast. HN03 also enhanced the functional traits of the soil, including defensive mechanism-related traits, and these traits were further enhanced by the combination of HN03 + wormcast.

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Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Cited by 86 publications

References 46 publications

Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01

Membrane disruption of Fusarium oxysporum f. sp. niveum induced by myriocin from Bacillus amyloliquefaciens LZN01

Responses of Soil Microbial Communities to the Invasion of Fusarium Oxysporum and Screening for Fusarium Antagonistic Microorganisms From Pathogenic Soil

The Soil Nutrient Environment Determines the Strategy by Which Bacillus velezensis HN03 Suppresses Fusarium wilt in Banana Plants

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