Comparative transcriptome analysis reveals the regulatory effects of acetylcholine on salt tolerance of Nicotiana benthamiana

Qin, Cheng; Ahanger, Mohammad Abass; Lin, Bo; Huang, Ziguang; Zhou, Jie; Ahmed, Nadeem; Ai, Suilong; Mustafa, N. S.; Ashraf, Muhammad; Zhang, Lixin

doi:10.1016/j.phytochem.2020.112582

Cited by 30 publications

(13 citation statements)

References 69 publications

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“…This strengthened the antioxidant system for better elimination of ROS, thereby mitigating oxidative effects on key macromolecular functions. Similarly to our findings, salinity stress-induced up-regulation of the antioxidant system has been reported [7,79,86]. Plants exhibiting higher antioxidant functioning show greater tolerance to stress [87], and upregulation of the antioxidant system due to applied MYO may have protected Chenopodium quinoa by maintaining low ROS levels and redox homeostasis, thereby protecting growth and development.…”

Section: Discussionsupporting

confidence: 89%

“…The decline in photosynthetic performance under salinity stress was attributed to altered K/Na [78]. The reduction in photosynthesis due to salinity stress could be due to the excess ROS accumulation [79] and lowered tissue water potential [80]. It has been concluded that salinity stress decreases chlorophyll biosynthesis by affecting the activity of key biosynthetic enzymes [81].…”

Section: Discussionmentioning

confidence: 99%

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Exogenous Myo-Inositol Alleviates Salt Stress by Enhancing Antioxidants and Membrane Stability via the Upregulation of Stress Responsive Genes in Chenopodium quinoa L.

Al-Mushhin

Qari

Fakhr

et al. 2021

Plants

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Myo-inositol has gained a central position in plants due to its vital role in physiology and biochemistry. This experimental work assessed the effects of salinity stress and foliar application of myo-inositol (MYO) on growth, chlorophyll content, photosynthesis, antioxidant system, osmolyte accumulation, and gene expression in quinoa (Chenopodium quinoa L. var. Giza1). Our results show that salinity stress significantly decreased growth parameters such as plant height, fresh and dry weights of shoot and root, leaf area, number of leaves, chlorophyll content, net photosynthesis, stomatal conductance, transpiration, and Fv/Fm, with a more pronounced effect at higher NaCl concentrations. However, the exogenous application of MYO increased the growth and photosynthesis traits and alleviated the stress to a considerable extent. Salinity also significantly reduced the water potential and water use efficiency in plants under saline regime; however, exogenous application of myo-inositol coped with this issue. MYO significantly reduced the accumulation of hydrogen peroxide, superoxide, reduced lipid peroxidation, and electrolyte leakage concomitant with an increase in the membrane stability index. Exogenous application of MYO up-regulated the antioxidant enzymes’ activities and the contents of ascorbate and glutathione, contributing to membrane stability and reduced oxidative damage. The damaging effects of salinity stress on quinoa were further mitigated by increased accumulation of osmolytes such as proline, glycine betaine, free amino acids, and soluble sugars in MYO-treated seedlings. The expression pattern of OSM34, NHX1, SOS1A, SOS1B, BADH, TIP2, NSY, and SDR genes increased significantly due to the application of MYO under both stressed and non-stressed conditions. Our results support the conclusion that exogenous MYO alleviates salt stress by involving antioxidants, enhancing plant growth attributes and membrane stability, and reducing oxidative damage to plants.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Exogenous Myo-Inositol Alleviates Salt Stress by Enhancing Antioxidants and Membrane Stability via the Upregulation of Stress Responsive Genes in Chenopodium quinoa L.

Al-Mushhin

Qari

Fakhr

et al. 2021

Plants

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“…Moreover, MdBZR1 and MdBZR1-like TFs enhance GA biosynthesis genes and improve salt tolerance in apple [161]. In addition, this trend is confirmed in Scots pine, in which acetylcholine upregulates GAs biosynthetic genes under salt stress conditions [133].…”

Section: Saline Stressmentioning

confidence: 76%

“…DA can also boosts GA 3 action in lettuce hypocotyls [131]. Finally, Acetylcholine (ACh), usually characterized as an animal hormone, plays a role in plant development, raising the content of free GAs in Scots pine seedlings [132] and upregulating the GAs signaling pathway of tobacco plants under salt stress [133]. Future research on the activity of these neurotransmitters in plants will probably increase the number of interactions with GAs.…”

Section: Interactions With Potential Pgrs or Relevant Compoundsmentioning

confidence: 99%

Interactions of Gibberellins with Phytohormones and Their Role in Stress Responses

Castro-Camba¹,

Sánchez²,

Vidal³

et al. 2022

Horticulturae

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Gibberellins are amongst the main plant growth regulators. Discovered over a century ago, the interest in gibberellins research is growing due to their current and potential applications in crop production and their role in the responses to environmental stresses. In the present review, the current knowledge on gibberellins’ homeostasis and modes of action is outlined. Besides this, the complex interrelations between gibberellins and other plant growth regulators are also described, providing an intricate network of interactions that ultimately drives towards precise and specific gene expression. Thus, genes and proteins identified as being involved in gibberellin responses in model and non-model species are highlighted. Furthermore, the molecular mechanisms governing the gibberellins’ relation to stress responses are also depicted. This review aims to provide a comprehensive picture of the state-of-the-art of the current perceptions of the interactions of gibberellins with other phytohormones, and their responses to plant stresses, thus allowing for the identification of the specific mechanisms involved. This knowledge will help us to improve our understanding of gibberellins’ biology, and might help increase the biotechnological toolbox needed to refine plant resilience, particularly under a climate change scenario.

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“…Plants upregulate their tolerance mechanisms to alleviate the damaging effects of ROS, and included in these key tolerance mechanisms are the antioxidant system, glyoxalase system, and osmolyte accumulation ( Ahmad et al., 2020 ). The enzymatic and non-enzymatic components of the antioxidant system work in close coordination to maintain the cellular ROS concentrations, and they also contribute to redox homeostasis, maintenance of enzyme activity, and photosynthesis ( Begum et al., 2020 ; Qin et al., 2021 ). The glyoxalase system consists of two key enzymes, i.e., glyoxalase I (Gly I) and glyoxalase II (Gly II), that act on MG to prevent its cytotoxic effects ( Ahmad et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Supplementation of nitric oxide and spermidine alleviates the nickel stress-induced damage to growth, chlorophyll metabolism, and photosynthesis by upregulating ascorbate–glutathione and glyoxalase cycle functioning in tomato

2022

Self Cite

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Experiments were conducted to evaluate the role of exogenously applied nitric oxide (NO; 50 µM) and spermidine (Spd; 100 µM) in alleviating the damaging effects of Ni (1 mM NiSO46H2O) toxicity on the growth, chlorophyll metabolism, photosynthesis, and mineral content in tomato. Ni treatment significantly reduced the plant height, dry mass, and the contents of glutamate 1-semialdehyde, δ-amino levulinic acid, prototoporphyrin IX, Mg–prototoporphyrin IX, total chlorophyll, and carotenoids; however, the application of NO and Spd alleviated the decline considerably. Supplementation of NO and Spd mitigated the Ni-induced decline in photosynthesis, gas exchange, and chlorophyll fluorescence parameters. Ni caused oxidative damage, while the application of NO, Spd, and NO+Spd significantly reduced the oxidative stress parameters under normal and Ni toxicity. The application of NO and Spd enhanced the function of the antioxidant system and upregulated the activity of glyoxalase enzymes, reflecting significant reduction of the oxidative effects and methylglyoxal accumulation. Tolerance against Ni was further strengthened by the accumulation of proline and glycine betaine due to NO and Spd application. The decrease in the uptake of essential mineral elements such as N, P, K, and Mg was alleviated by NO and Spd. Hence, individual and combined supplementation of NO and Spd effectively alleviates the damaging effects of Ni on tomato.

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Comparative transcriptome analysis reveals the regulatory effects of acetylcholine on salt tolerance of Nicotiana benthamiana

Cited by 30 publications

References 69 publications

Exogenous Myo-Inositol Alleviates Salt Stress by Enhancing Antioxidants and Membrane Stability via the Upregulation of Stress Responsive Genes in Chenopodium quinoa L.

Exogenous Myo-Inositol Alleviates Salt Stress by Enhancing Antioxidants and Membrane Stability via the Upregulation of Stress Responsive Genes in Chenopodium quinoa L.

Interactions of Gibberellins with Phytohormones and Their Role in Stress Responses

Supplementation of nitric oxide and spermidine alleviates the nickel stress-induced damage to growth, chlorophyll metabolism, and photosynthesis by upregulating ascorbate–glutathione and glyoxalase cycle functioning in tomato

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