Comparative Transcriptome Analysis to Reveal Genes Involved in Wheat Hybrid Necrosis

Abstract

Wheat hybrid necrosis is an interesting genetic phenomenon that is found frequently and results in gradual death or loss of productivity of wheat. However, the molecular basis and mechanisms of this genetic phenomenon are still not well understood. In this study, the transcriptomes of wheat hybrid necrosis F1 and its parents (Neimai 8 and II469) were investigated using digital gene expression (DGE). A total of 1300 differentially expressed genes were identified, indicating that the response to hybrid necrosis in wheat is complicated. The assignments of the annotated genes based on Gene Ontology (GO) revealed that most of the up-regulated genes belong to “universal stress related”, “DNA/RNA binding”, “protein degradation” functional groups, while the down-regulated genes belong to “carbohydrate metabolism” and “translation regulation” functional groups. These findings suggest that these pathways were affected by hybrid necrosis. Our results provide preliminarily new insight into the underlying molecular mechanisms of hybrid necrosis and will help to identify important candidate genes involved in wheat hybrid necrosis.

A CC-NBS-LRR gene induces hybrid lethality in cotton

“…NBS-LRR activation results in an elevated immune response, characterized by the activation of defense-associated genes and the generation of ROS and hypersensitive responses (Spoel and Dong, 2012). In our present study, we found that autoimmunity was associated with hybrid lethality, as has been reported in a number of previous studies (Hatano et al , 2012; Chen et al , 2014; Zhang et al , 2014). Based on our experimental and transcriptome data, the mechanism for induction of autoimmunity and plant death by Le 4 in the interspecific F 1 hybrid between R4-4 and upland cotton is illustrated in Fig.…”

Exploring the genes involved in biosynthesis of dihydroquercetin and dihydromyricetin in Ampelopsis grossedentata

“…Total RNA purity and concentration of each sample were measured using the NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA), and RNA integrity then assessed with the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Only those samples with an RNA integrity number (RIN) ≥ 7 were retained for subsequent analysis 36 .…”

ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

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