Comparative Transcriptome and Hormone Analysis of Mature Leaves and New Shoots in Tea Cuttings (Camellia sinensis) among Three Cultivars with Different Rooting Abilities

Fan, Kai; Shi, Yujie; Luo, Danni; Qian, Wenjun; Shen, Jiazhi; Ding, Shibo; Ding, Zhaotang; Wang, Yu

doi:10.1007/s00344-021-10478-0

Cited by 10 publications

(6 citation statements)

References 54 publications

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“…These findings indicate that the formation of ARs induced by hypocotyl shading may be related to the accumulation of JA-ILE and enhanced signaling. Meanwhile, the significant accumulation of JA-ILE and JA, accompany the upregulation of MYC2 induced by exogenous melatonin may further activate the expression of JA-responsive genes, thereby significantly promoting AR formation, in accordance with the finding that JA acts as a accelerator of AR formation in tea plants (Fan et al, 2021).…”

Section: Discussionsupporting

confidence: 83%

“…ABA 8’-hydroxylase catalyzes the oxidative degradation of ABA, while β-glucosidase (β-Glu) can hydrolyze ABA gluconate into bioactive ABA. Studies on tea plants showed that ABA content was higher in the easy-to-root cultivar, which indicate that ABA is involved in the occurrence of AR ( Fan et al., 2021 ). In our study, the expression of β-Glu (CsaV3_1G042500, CsaV3_1G042530 and CsaV3_1G042540) were significantly upregulated by hypocotyl shading and melatonin further upregulated these genes under hypocotyl shading conditions, while ABA 8′-hydroxylase was inhibited by melatonin ( Figure 6A , Supplementary Table S1 ).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls

Wang

Zhao

et al. 2022

Front. Plant Sci.

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Melatonin, a multi-regulatory molecule, stimulates root generation and regulates many aspects of plant growth and developmental processes. To gain insight into the effects of melatonin on adventitious root (AR) formation, we use cucumber seedings subjected to one of three treatments: EW (hypocotyl exposed and irrigated with water), SW (hypocotyl shaded and irrigated with water) and SM (hypocotyl shaded and irrigated with 100 µM melatonin). Under shaded conditions, melatonin induced significant AR formation in the hypocotyl. To explore the mechanism of this melatonin-induced AR formation, we used transcriptome analysis to identify 1296 significant differentially expressed genes (DEGs). Comparing SM with SW, a total of 774 genes were upregulated and 522 genes were downregulated. The DEGs were classified among different metabolic pathways, especially those connected with the synthesis of secondary metabolites, with hormone signal transduction and with plant-pathogen interactions. Analyses indicate exogenous melatonin increased contents of endogenous auxin, jasmonic acid, salicylic acid, cytokinin and abscisic acid levels during AR formation. This study indicates melatonin promotes AR formation in cucumber seedings by regulating the expressions of genes related to hormone synthesis, signaling and cell wall formation, as well as by increasing the contents of auxin, cytokinin, jasmonic acid, salicylic acid and abscisic acid. This research elucidates the molecular mechanisms of melatonin’s role in promoting AR formation in the hypocotyl of cucumber seedings under shaded conditions.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls

Wang

Zhao

et al. 2022

Front. Plant Sci.

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“…ABA was considered an inhibitor of the rooting of Eucalyptus globulus [17], but in this study, its content also increased first and then decreased, increasing during the rooting period (Figure 2). In addition, in previous studies, ABA had the highest content under blue light, which was most favorable for rooting [4] and was highest in the new shoots of the easily rooted C. sinensis 'Baihaozao' [11]. These differences may be related to the different ABA distribution in different tissues, and the ABA metabolism in mature leaves is not the same as the base of cuttings.…”

Section: Blue Light Induced Adventitious Root Development By Affectin...mentioning

confidence: 95%

“…In addition, the ratio of ICA/GA9 showed a trend of first increasing and then decreasing (Figure 2). Fan et al's (2021) study showed that the IAA/GA3 ratio in the new shoots of the best rooting C. sinensis 'Baihaozao' was significantly higher than other varieties [11], which also explains the reason for the increase in ICA/GA9 ratio in the early stage (Figure 2). JA can rapidly accumulate in the rooting induction stage of Petunia hybrida cuttings [19], which indicates that JA can promote adventitious root formation.…”

Section: Blue Light Induced Adventitious Root Development By Affectin...mentioning

confidence: 97%

Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (Camellia sinensis (L.) Kuntze)

et al. 2023

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C. sinensis is an economically important crop for tea production that experiences increasing demand and good export potential. Therefore, crops need to be expanded, and high-quality planting material is required. Vegetative propagation by cuttings is the prevalent method; therefore, this paper explored its optimization potential modeled on cultivar ‘Jiukengzao’. This study wanted to deeply explore blue-light-induced adventitious root formation and development of tea cuttings, so we conducted short-term (0 h, 8 h, and 16 h) and long-term (30 d, 60 d, and 90 d) time-course analyses on tea cutting seedlings. Short-term, full-length transcriptome analysis showed that the expression of genes related to plant hormone signal transduction and auxin transport was highest at 16 h. Sixteen hours of light was considered as suitable for adventitious root growth and development of tea cuttings. Long-term phytohormone analysis showed that the trend of indole-3-carboxylic acid (ICA) change was: 60 d > 90 d > 30 d. Long-term, full-length transcriptome analysis showed that the gene expression trends in K2, K5, K6, and K8 clusters were: 90 d > 60 d > 30 d, and the opposite was observed in K1, K4, and K11 clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the genes in these seven clusters are involved in “plant hormone signal transduction (ko04075)”. This includes auxin early responsive protein AUX/IAA, auxin response factor ARF, auxin-responsive protein SAUR, etc. In addition, genes related to auxin transport and synthesis were identified as PIN1, 3, 4, PILS2, 6, 7, flavin-containing monooxygenase YUC9, and YUC10, and the expression trend of these genes was mostly consistent with the change trend of ICA content. This study further explained the molecular mechanism of blue-light-induced adventitious root formation and development of tea cuttings. It is recommended that blue light can be used to promote the adventitious root growth and development of tea cuttings in practical production.

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“…On the other hand, LAX3 is also active during adventitious root formation ( Rovere et al, 2016 ). In addition, Fan et al (2021) showed that the AUX1/LAX family was considered to be the key gene for adventitious root formation by studying the cuttings of three varieties of tea with different rooting abilities. Meanwhile, Yousef et al (2021) investigated the effect of different light qualities on graft union formation in grafted tomatoes and showed that the expression levels of both AUX1 and ARF were higher in blue LEDs than in red LEDs and white fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Red and Blue Light Affect the Formation of Adventitious Roots of Tea Cuttings (Camellia sinensis) by Regulating Hormone Synthesis and Signal Transduction Pathways of Mature Leaves

et al. 2022

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Light is an important environmental factor which affects plant growth, through changes of intensity and quality. In this study, monochromatic white (control), red (660 nm), and blue (430 nm) light-emitting diodes (LEDs) were used to treat tea short cuttings. The results showed the most adventitious roots in blue light treated tea cuttings, but the lowest roots in that treated by red light. In order to explore the molecular mechanism of light quality affecting adventitious root formation, we performed full-length transcriptome and metabolome analyses of mature leaves under three light qualities, and then conducted weighted gene co-expression network analysis (WGCNA). Phytohormone analysis showed that Indole-3-carboxylic acid (ICA), Abscisic acid (ABA), ABA-glucosyl ester (ABA-GE), trans-Zeatin (tZ), and Jasmonic acid (JA) contents in mature leaves under blue light were significantly higher than those under white and red light. A crosstalk regulatory network comprising 23 co-expression modules was successfully constructed. Among them, the “MEblue” module which had a highly positive correlation with ICA (R = 0.92, P = 4e-04). KEGG analysis showed that related genes were significantly enriched in the “Plant hormone signal transduction (ko04075)” pathway. YUC (a flavin-containing monooxygenase), AUX1, AUX/IAA, and ARF were identified as hub genes, and gene expression analysis showed that the expression levels of these hub genes under blue light were higher than those under white and red light. In addition, we also identified 6 auxin transport-related genes, including PIN1, PIN3, PIN4, PILS5, PILS6, and PILS7. Except PILS5, all of these genes showed the highest expression level under blue light. In conclusion, this study elucidated the molecular mechanism of light quality regulating adventitious root formation of tea short cutting through WGCNA analysis, which provided an innovation for “rapid seedling” of tea plants.

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Comparative Transcriptome and Hormone Analysis of Mature Leaves and New Shoots in Tea Cuttings (Camellia sinensis) among Three Cultivars with Different Rooting Abilities

Cited by 10 publications

References 54 publications

Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls

Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls

Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (Camellia sinensis (L.) Kuntze)

Red and Blue Light Affect the Formation of Adventitious Roots of Tea Cuttings (Camellia sinensis) by Regulating Hormone Synthesis and Signal Transduction Pathways of Mature Leaves

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