Comparative Transcriptome and WGCNA&amp;nbsp;Reveal Candidate Genes Involved in Petaloid Stamens in Paeonia Lactiflora

Fan, Yongming; Yanyi, Zheng; Silva, Jaime A. Teixeira da; Yu, Xiaonan

doi:10.21203/rs.3.rs-95599/v1

Cited by 2 publications

(1 citation statement)

References 60 publications

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“…Lin et al (2018) selected numerous genes involved in hormone signal transduction pathways and transcription factors as candidate genes for the formation of N. nucifera stamen petals through comparative transcriptome sequencing [29]. Fan et al (2021) performed transcriptomic analysis of single-petal and double-petal Paeonia lactiflora flowers and screened out 18 candidate genes involved in the formation and development of petaloid stamens [30]. The study further proposed a hypothetical model of gene expression network regulating the development of petaloid stamens.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics analyses reveal the key genes involved in stamen petaloid formation in Alcea rosea L.

Luo,

Li,

Yin

et al. 2024

BMC Plant Biol

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Alcea rosea L. is a traditional flower with a long cultivation history. It is extensively cultivated in China and is widely planted in green belt parks or used as cut flowers and potted ornamental because of its rich colors and flower shapes. Double-petal A. rosea flowers have a higher aesthetic value compared to single-petal flowers, a phenomenon determined by stamen petaloid. However, the underlying molecular mechanism of this phenomenon is still very unclear. In this study, an RNA-based comparative transcriptomic analysis was performed between the normal petal and stamen petaloid petal of A. rosea. A total of 3,212 differential expressed genes (DEGs), including 2,620 up-regulated DEGs and 592 down-regulated DEGs, were identified from 206,188 unigenes. Numerous DEGs associated with stamen petaloid were identified through GO and KEGG enrichment analysis. Notably, there were 63 DEGs involved in the plant hormone synthesis and signal transduction, including auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinosteroid, jasmonic acid, and salicylic acid signaling pathway and 56 key transcription factors (TFs), such as MADS-box, bHLH, GRAS, and HSF. The identification of these DEGs provides an important clue for studying the regulation pathway and mechanism of stamen petaloid formation in A. rosea and provides valuable information for molecular plant breeding.

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Section: Introductionmentioning

confidence: 99%

Transcriptomics analyses reveal the key genes involved in stamen petaloid formation in Alcea rosea L.

Luo,

Li,

Yin

et al. 2024

BMC Plant Biol

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Integrated SMRT Technology with UMI RNA-Seq Reveals the Hub Genes in Stamen Petalody in Camellia oleifera

Yang

Gao

et al. 2021

Forests

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Male sterility caused by stamen petalody is a key factor for a low fruit set rate and a low yield of Camellia oleifera but can serve as a useful genetic tool because it eliminates the need for artificial emasculation. However, its molecular regulation mechanism still remains unclear. In this study, transcriptome was sequenced and analyzed on two types of bud materials, stamen petalody mutants and normal materials, at six stages of stamen development based on integrated single-molecule real-time (SMRT) technology with unique molecular identifiers (UMI) and RNA-seq technology to identify the hub genes responsible for stamen petalody in C. oleifera. The results show that a large number of alternative splicing events were identified in the transcriptome. A co-expression network analysis of MADSs and all the differentially expressed genes between the mutant stamens and the normal materials showed that four MADS transcription factor genes, CoSEP3.1, CoAGL6, CoSEP3.2, and CoAP3, were predicted to be the hub genes responsible for stamen petalody. Among these four, the expression patterns of CoAGL6 and CoSEP3.2 were consistently high in the mutant samples, but relatively low in the normal samples at six stages, while the patterns of CoSEP3.1 and CoAP3 were initially low in mutants and then were upregulated during development but remained relatively high in the normal materials. Furthermore, the genes with high connectivity to the hub genes showed significantly different expression patterns between the mutant stamens and the normal materials at different stages. qRT-PCR results showed a similar expression pattern of the hub genes in the RNA-seq. These results lay a solid foundation for the directive breeding of C. oleifera varieties and provide references for the genetic breeding of ornamental Camellia varieties.

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Comparative Transcriptome and WGCNA Reveal Candidate Genes Involved in Petaloid Stamens in Paeonia Lactiflora

Cited by 2 publications

References 60 publications

Transcriptomics analyses reveal the key genes involved in stamen petaloid formation in Alcea rosea L.

Transcriptomics analyses reveal the key genes involved in stamen petaloid formation in Alcea rosea L.

Integrated SMRT Technology with UMI RNA-Seq Reveals the Hub Genes in Stamen Petalody in Camellia oleifera

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