Comparative transcriptome expression analysis in susceptible and resistant potato (Solanum tuberosum) cultivars to common scab (Streptomyces scabies) revealed immune priming responses in the incompatible interaction

Abstract

Common scab disease in potato has become a widespread issue in major potato production areas, leading to increasing economic losses. Varietal resistance is seen as a viable and long-term scab management strategy. However, the genes and mechanisms of varietal resistance are unknown. In the current study, a comparative RNA transcriptome sequencing and differential gene signaling and priming sensitization studies were conducted in two potato cultivars that differ by their response to common scab ( Streptomyces scabies) , for unraveling the genes and pathways potentially involved in resistance within this pathosystem. We report on a consistent and contrasted gene expression pattern from 1,064 annotated genes differentiating a resistant (Hindenburg) and a susceptible (Green Mountain) cultivars, and identified a set of 273 co-regulated differentially expressed genes in 34 pathways that more likely reflect the genetic differences of the cultivars and metabolic mechanisms involved in the scab pathogenesis and resistance. The data suggest that comparative transcriptomic phenotyping can be used to predict scab lesion phenotype in breeding lines using mature potato tuber. The study also showed that the resistant cultivar, Hindenburg, has developed and maintained a capacity to sense and prime itself for persistent response to scab disease over time, and suggests an immune priming reaction as a mechanism for induced-resistance in scab resistant potato cultivars. The set of genes identified, described, and discussed in the study paves the foundation for detailed characterizations towards tailoring and designing procedures for targeted gene knockout through gene editing and phenotypic evaluation.

Habituation to thaxtomin A increases resistance to common scab in ‘Russet Burbank’ potato

“…For instance, increased resistance to common scab could be due to enhanced or constitutive activation of defense- or stress-related pathways especially in tubers. Recent work using transcriptomic analysis that compared gene expression between common scab resistant and susceptible potato cultivars has revealed that immune priming may indeed be involved in the induced resistance in the common scab resistant cultivar Hindenburg [ 37 ]. However, there is wide evidence that the activation of defense mechanisms is a high energy demanding process that can lead to reduction in plant growth (rev.…”

Quantitative Trait Locus Mapping for Common Scab Resistance in a Tetraploid Potato Full-Sib Population

“…Although the list of candidate genes is still hypothetical, several transcripts encoding related protein isoforms (e.g. MYB, WRKY, LRR receptor-like serine/threonine-protein kinase) were found to be differentially expressed between the resistant 'Hindenburg' and susceptible 'Green Mountain' cultivars inoculated with S. scabies (Fofana et al, 2020). Here, we detected a single QTL consistently in two out of three environments, that explains up to 30% of the variation for lesion index.…”

Assessment of Soil Contamination by Gas Cloud Generated from Chemical Fire Using Metabolic Profiling and Associated Bacterial Communities

“…It was expected that Arthrobacter would break down the aromatic compound when contaminated with toluene. The Streptomyces scabiei attend the steroid degradation pathway [39]. Bradyrhizobium japonicum was reported as toluene degrader [40].…”