Comparative transcriptome profiling analysis provides insight into the mechanisms for sugar change in Chinese jujube (<i>Ziziphus jujuba</i> Mill.) under rain‐proof cultivation

Ji, Qing; Wang, Ruifang; Chen, Kai; Xie, Zhengwan; Sun-yang, LI; Wang, Dawei; Zhang, Ao; Xu, Yuan; Li, Shenghui; Cui, J. F.; Li, Sha; Zhou, Jun; Li-hu, Wang

doi:10.1002/tpg2.20341

Cited by 2 publications

(1 citation statement)

References 72 publications

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“…Hexokinase-like (HKL) is an isoenzyme of HXK , and although the function of HLK is unknown, defective phosphorylation of AtHKL1 can negatively regulate plant growth ( Karve et al., 2008 ). Recent studies also indicate that HXK effect root development ( Granot et al., 2014 ), leaf senescence ( Swartzberg et al., 2011 ), regulates photosynthesis ( Roth et al., 2019 ), pollen germination ( Karni and Aloni, 2002 ), and sugar content during fruit development ( Ji et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill.

Tong,

Liao,

et al. 2024

Front. Plant Sci.

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Hexokinase (HXK) plays a crucial role in plants, catalyzing the phosphorylation of hexose substances, which is one of the key steps in sugar metabolism and energy production. While HXK genes have been well-studied in model plants, the evolutionary and functional characteristics of HXK gene family in jujube is unknow. In this study, the HXK gene family members were identified by bioinformatics methods, the key members regulating glucose metabolism were identified by transcriptome data, and finally the function of the key genes was verified by instantaneous and stable genetic transformation. Our results showed that seven HXK genes were identified in the jujube genome, all of which were predict located in the chloroplast and contain Hexokinase-1 (PF00349) and Hexokinase-2 (PF03727) conserved domains. Most of HXK proteins were transmembrane protein with stable, lipid-soluble, hydrophilic. The secondary structure of ZjHXK proteins main α-helix, and contains two distinct tertiary structure. All ZjHXK genes contain nine exons and eight introns. Predictions of cis-regulatory elements indicate that the promoter region of ZjHXK contains a large number of MeJA responsive elements. Finally, combined with the analysis of the relationship between the expression and glucose metabolism, found that ZjHXK5 and ZjHXK6 may the key genes regulating sugar metabolism. Transient overexpression of ZjHXK5 and ZjHXK6 on jujube, or allogeneic overexpression of ZjHXK5 and ZjHXK6 on tomato would significantly reduce the content of total sugar and various sugar components. Transient silencing of ZjHXK5 and ZjHXK6 genes results in a significant increase in sucrose and total sugar content. Interestingly, the expression of ZjHXK5 and ZjHXK6 were also affected by methyl jasmonate.

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Section: Introductionmentioning

confidence: 99%

ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill.

Tong,

Liao,

et al. 2024

Front. Plant Sci.

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Genome-wide identification and expression analysis of C2H2 zinc finger proteins in Chinese jujube (Ziziphus jujuba Mill.) in different fruit development stages and under different levels of water stress

Zhengwan,

Qing,

Wang

et al. 2024

PeerJ

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The C2H2 type zinc finger proteins (C2H2-ZFPs) are prevalent motifs found widely across the eukaryotic kingdom, playing crucial roles in various biological processes, including stress responses and plant growth and development. To date, C2H2-ZFPs have been identified in many plant systems, but there have been no reports in Chinese jujube (Ziziphus jujuba Mill.). In this study, a total of 77 ZjC2H2-ZFPs were identified in Chinese jujube and classified into three groups, with set A containing 29 genes, set B containing two genes, and set C containing 46 genes. The set A group genes were further divided into three groups: A1, A2, and A4 (with no member of the A3 subgroup found in jujube). The set C C2H2-ZFPs genes were also further divided into three groups: C1 containing 24 genes, C2 with two genes, and C3 with three genes. These ZjC2H2-ZFPs were distributed on 12 chromosomes and one tandem duplicated pair of ZjC2H2-ZFPs was found on chromosome 4 (ZjC2H2-21 with ZjC2H2-22). Notably, the 77 ZjC2H2-ZFPs identified in this study lacked finger clusters comprising 10 or more repeats. The structure and protein motif analysis of ZjC2H2-ZFPs showed that most C1 subgroup members were enriched with ‘QALGGH’ motif zinc finger helices and the A1a ZjC2H2-ZFPs contained highly conserved ‘SATALLQKAAQMGS’ residues in jujube. A unique finding was the discovery of a conserved non-finger domain (PCYCC motif) in A1 group members, absent in other ZjC2H2-ZFPs and unreported in other species. The enzyme activity of jujube leaves under different water stress treatments were measured, and the results showed that as the degree of water stress increased, the activity of SOD enzymes and H2O2 content also increased. The POD enzyme activity levels of different treatment groups were CK>MS>SS>LS. The levels of malondialdehyde (MDA) content observed under various treatments were notably higher and the proline content was lower in comparison to the control group (CK). Differential expression of ZjC2H2-ZFPs and specific responses were analyzed under water stress and different fruit development stages of jujube using RNA-Seq data. The correlation between expression patterns and protective enzyme activities under water stress was also examined. The results indicated that the expression levels of different ZjC2H2-ZFPs varied. A further protein interaction analysis indicated that ZjC2H2-ZFPs serve as pivotal transcriptional regulators with diverse functions, encompassing DNA or RNA binding and participation in protein interactions, with ZjC2H2-20, ZjC2H2-36, and ZjC2H2-57 being potential key players in these regulatory processes. Their roles appear particularly crucial in responding to abiotic stresses like water stress and regulating plant hormones. This study provides valuable insights into understanding stress responses and enhancing the quality of Chinese jujube during breeding.

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Comparative transcriptome profiling analysis provides insight into the mechanisms for sugar change in Chinese jujube (Ziziphus jujuba Mill.) under rain‐proof cultivation

Cited by 2 publications

References 72 publications

ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill.

ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill.

Genome-wide identification and expression analysis of C2H2 zinc finger proteins in Chinese jujube (Ziziphus jujuba Mill.) in different fruit development stages and under different levels of water stress

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