Comparative transcriptome profiling reveals that brassinosteroid-mediated lignification plays an important role in garlic adaption to salt stress

Kong, Qiusheng; Mostafa, Hassan H. A.; Yang, Wenlong; Wang, Jinglei; Nuerawuti, Maierdan; Wang, Yang; Song, Jiangping; Zhang, Xiaohui; Ma, Longchuan; Wang, Haiping; Li, Xixiang

doi:10.1016/j.plaphy.2020.11.033

Cited by 40 publications

(24 citation statements)

References 29 publications

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“…Phenylpropanoid biosynthesis is closely associated with plant responses to abiotic stresses, especially excessive salinity. Several studies revealed that the phenylpropanoid biosynthesis pathway is important for the salt tolerance of diverse plant species such as bread wheat [71], garlic [72], strawberry [73] and date palm (Phoenix dactylifera L.) [74]. In this study, 'phenylpropanoid biosynthesis' was an enriched KEGG pathway among the DEGs, including those associated with cinnamyl-alcohol dehydrogenase, coniferyl-aldehyde dehydrogenase, and POD, all of which affect lignin synthesis.…”

Section: Discussionmentioning

confidence: 76%

Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Zhang

Xia

et al. 2021

Biotechnology & Biotechnological Equipment

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a cereals germplasm Resources innovation laboratory, college of agriculture, national coarse cereals engineering Research center, heilongjiang Bayi agricultural university, Daqing, heilongjiang, pR china; b Bean crops laboratory, crop Resources institute of heilongjiang academy of agricultural Sciences, harbin, heilongjiang, pR china; c cereals germplasm Resources innovation laboratory, college of agriculture, national coarse cereals engineering Research center, Daqing, heilongjiang, pR china ABSTRACT Salt stress is an important factor restricting crop growth. Thus, genes related to plant responses to high salinity at the sprout stage of common bean (Phaseolus vulgaris) should be identified and characterized. In this study, on the basis of the common bean germination index, 0.4% NaCl and day 5 of the incubation period were selected as the optimal salt concentration and sampling time, respectively. Salt-tolerant and salt-sensitive plant materials suitable for the local region were selected as representative common bean materials with contrasting salt-related phenotypes. A total of 441 differentially expressed genes (DEGs) were identified by analyzing the generated RNA-seq data. The enriched Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways indicated the DEGs were associated with oxidoreductase activities and phenylpropanoid biosynthesis. Furthermore, the superoxide dismutase, peroxidase, and catalase activities and the lignin content, which are related to oxidoreductase activities and phenylpropanoid biosynthesis, were revealed to be significantly associated with common bean salt tolerance. The six DEGs with salt tolerance-related Arabidopsis thaliana homologs may be important candidate genes mediating the salt stress responses of common bean during the sprout stage.

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Section: Discussionmentioning

confidence: 76%

Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Zhang

Xia

et al. 2021

Biotechnology & Biotechnological Equipment

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“…Gene-specific primers were designed using Primer Premier 5.0 software ( Supplementary Table 1 ). The qRT-PCR experiment was performed using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific) Kit and was completed on the Applied Biosystems 7500 fast real-time PCR system using actin as a reference gene ( 14 ). The 2 −ΔΔCt method was used to calculate the expression of differential genes, and each treatment was repeated 3 times ( 31 ).…”

Section: Methodsmentioning

confidence: 99%

“…At present, the transcriptome of garlic has been sequenced and reassembled ( 13 ). Kong et al ( 14 ) studied the long-term adaptation response to salt stress during garlic growth and found that brassinosteroid might regulate lignin biosynthesis at the transcriptional level. Rukmankesh et al ( 15 ) studied the transcriptomics of snow mountain garlic, screened out candidate genes related to organic sulfur metabolism in garlic bulbs, and analyzed the highly expressed genes involved in photosynthesis in garlic leaves.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome in Combination Proteome Unveils the Phenylpropane Pathway Involved in Garlic (Allium sativum) Greening

Niu

et al. 2021

Front. Nutr.

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Garlic (Allium sativum) is an important vegetable crop that is widely used in cooking and medicine. The greening phenomenon of garlic severely decreases the quality of garlic and hinders garlic processing. To study the mechanism of garlic greening, comprehensive full-length transcript sets were constructed. We detected the differences in greening between Pizhou (PZ) garlic and Laiwu (LW) garlic that were both stored at −2.5°C and protected from light at the same time. The results showed that 60,087 unigenes were respectively annotated to the NR, KEGG, GO, Pfam, eggNOG and Swiss Prot databases, and a total of 30,082 unigenes were annotated. The analysis of differential genes and differential proteins showed that PZ garlic and LW garlic had 923 differentially expressed genes (DEGs), of which 529 genes were up regulated and 394 genes were downregulated. Through KEGG and GO enrichment analysis, it was found that the most significant way of enriching DEGs was the phenylpropane metabolic pathway. Proteomics analysis found that there were 188 differentially expressed proteins (DAPs), 162 up-regulated proteins, and 26 down-regulated proteins between PZ garlic and LW garlic. The content of 10 proteins related to phenylpropanoid biosynthesis in PZ garlic was significantly higher than that of LW garlic. This study explored the mechanisms of garlic greening at a molecular level and further discovered that the formation of garlic green pigment was affected significantly by the phenylpropanoid metabolic pathway. This work provided a theoretical basis for the maintenance of garlic quality during garlic processing and the future development of the garlic processing industries.

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“…However, the differentiation switch of xylem observed in drought conditions mediated by ABA is not replicated during the NaCl treatment (Bloch, Puli et al 2019), showing other unknown elements must play a part in the salt response. At the same time, it has been shown, that high salinity stress induces an increased lignification of the xylem tissues in Tomato plants (Sánchez-Aguayo, Rodríguez-Galán et al 2004) and other plant species (Neves, Marchiosi et al 2010, FERNANDEZ-GARCIA, HERNANDEZ et al 2011, Oliveira, Mota et al 2020, Kong, Mostafa et al 2021). However, not much is known on the molecular mechanism of salt-induced over-lignification.…”

Section: Salt Stressmentioning

confidence: 98%

Understanding the Root Xylem Plasticity for Designing Resilient Crops

Cornelis

Hazak

2021

Preprint

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Xylem is a main road in plant long-distance communication. Through xylem plants transport water, minerals and myriad of signaling molecules. With the onset during early embryogenesis, the development of xylem tissues relays on hormone gradients, activity of unique transcription factors, distribution of mobile miRNAs and receptor-ligand pathways. These regulatory mechanisms are often interconnected and all together contribute to the plasticity of water conducting tissue. Remarkably, root xylem carries water to all above-ground organs and therefore influences all aspects of plant growth. Because of the global warming and increasing water deficit, we need to come up with solutions for the crops of the future. It is clear that structure of water conducting elements directly impacts water transport within the plant. Among plant pathogens- vascular wilts attacking xylem -are the most harmful. Our knowledge about xylem anatomy and rewiring ability could bring the solutions against these diseases. In this review we summarize the recent findings on the molecular mechanisms of xylem formation with a special attention to the cellular changes, and cell wall rearrangements that are necessary to create functional capillaries. We emphasize the impact of abiotic factors and pathogens on xylem plasticity and discuss multidisciplinary approach to model xylem in crops.

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Comparative transcriptome profiling reveals that brassinosteroid-mediated lignification plays an important role in garlic adaption to salt stress

Cited by 40 publications

References 29 publications

Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Transcriptome in Combination Proteome Unveils the Phenylpropane Pathway Involved in Garlic (Allium sativum) Greening

Understanding the Root Xylem Plasticity for Designing Resilient Crops

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