Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes

Samal, Parshuram; Molla, Kutubuddin A.; Bal, A. R.; Ray, Soham; Swain, Harekrushna; Khandual, Ansuman; Sahoo, Pritiranjan; Behera, Monalisa; Jaiswal, Sarika; Iquebal, Mir Asif; Chakraborti, Mridul; Behera, Lambodar; Kar, Meera Kumari; Mukherjee, Arup

doi:10.1007/s00709-021-01637-x

Cited by 16 publications

(15 citation statements)

References 70 publications

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“…To decipher the differential gene expression responses during the R. solani infection, a time-series RNA-seq analysis (i.e., 0, 24, and 48 h) was performed, and a total of 7,950 and 7,080 DEGs in GD66 and Lemont were identified, respectively. Our results are consistent with those by Samal et al (2022), who detected more DEGs in the resistant genotype CR1014 than that in the susceptible genotype Swarna-Sub1. Similarly, stronger and earlier responses to pathogen invasion at the transcriptome level have been found in the resistant grapevine specie Vitis riparia infected with Plasmopara viticola (Polesani et al, 2010).…”

Section: Degs Between Sheath Blight-resistant and Sheath Blight-susce...supporting

confidence: 93%

Cytological observation and transcriptome analysis reveal dynamic changes of Rhizoctonia solani colonization on leaf sheath and different genes recruited between the resistant and susceptible genotypes in rice

et al. 2022

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Sheath blight, caused by Rhizoctonia solani, is a big threat to the global rice production. To characterize the early development of R. solani on rice leaf and leaf sheath, two genotypes, GD66 (a resistant genotype) and Lemont (a susceptible genotype), were observed using four cytological techniques: the whole-mount eosin B-staining confocal laser scanning microscopy (WE-CLSM), stereoscopy, fluorescence microscopy, and plastic semi-thin sectioning after in vitro inoculation. WE-CLSM observation showed that, at 12 h post-inoculation (hpi), the amount of hyphae increased dramatically on leaf and sheath surface, the infection cushions occurred and maintained at a huge number from about 18 to 36 hpi, and then the infection cushions disappeared gradually from about 42 to 72 hpi. Interestingly, R. solani could not only colonize on the abaxial surfaces of leaf sheath but also invade the paraxial side of the leaf sheath, which shows a different behavior from that of leaf. RNA sequencing detected 6,234 differentially expressed genes (DEGs) for Lemont and 7,784 DEGs for GD66 at 24 hpi, and 2,523 DEGs for Lemont and 2,719 DEGs for GD66 at 48 hpi, suggesting that GD66 is recruiting more genes in fighting against the pathogen. Among DEGs, resistant genes, such as OsRLCK5, Xa21, and Pid2, displayed higher expression in the resistant genotype than the susceptible genotype at both 24 and 48 hpi, which were validated by quantitative reverse transcription–PCR. Our results indicated that the resistance phenotype of GD66 was the consequence of recruiting a series of resistance genes involved in different regulatory pathways. WE-CLSM is a powerful technique for uncovering the mechanism of R. solani invading rice and for detecting rice sheath blight–resistant germplasm.

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Section: Degs Between Sheath Blight-resistant and Sheath Blight-susce...supporting

confidence: 93%

Cytological observation and transcriptome analysis reveal dynamic changes of Rhizoctonia solani colonization on leaf sheath and different genes recruited between the resistant and susceptible genotypes in rice

et al. 2022

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“…The synthesis of an important rice flavonoid, i.e., sakuranetin, is also dependent on CHS activation (Koley et al, 2022). However, Samal et al (2022) recorded a significantly higher accumulation of CHS transcripts in CR 1014 (the international tolerant variety) than in the Swarna‐Sub1 (the susceptible variety).…”

Section: Discussionmentioning

confidence: 99%

“…SA promotes the increase in lignin synthesis through the shikimic acid pathway, which might act as a barrier and offer some degree of protection against fungal penetration (Anderson et al, 2016). Samal et al (2022) discovered that R. solani infection reduced lignin production in susceptible plant material.…”

Section: Discussionmentioning

confidence: 99%

Physio‐biochemical and histological alterations in response to sheath blight disease of rice (Oryza sativaL.)

Chhabra

Sharma

Hunjan

et al. 2022

Journal of Phytopathology

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Rice (Oryza sativa L.), belonging to the family Poaceae, is the staple food of more than 50% of the world's population. Rice is one of the ancient cereal crops being grown in 117 countries, hence called "Global Grain" (Singh et al., 2019). Over two billion people in Asia derive 80% of their energy needs from rice as a staple food crop (Chaudhary & Tran, 2001). Rice cultivation accounts for 23.3% of gross cropped area in India, accounting for 46% of total cereal production (FAO, 2021). As per the statistical data available, India is

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“…It has been documented (in rice) that the resistance response mechanism against one particular pathotype may differ from the resistance response under field conditions in the presence of multiple pathotypes 37 . In this study we identified three bins of 0.86, 2.03 and 4.68 Mb on chromosome 5 that were effective against pathotype AMP-048 from Arkansas.…”

Section: Discussionmentioning

confidence: 99%

The inheritance of anthracnose (Colletotrichum sublineola) resistance in sorghum differential lines QL3 and IS18760

Cuevas

Cruet-Burgos

Prom

et al. 2021

Sci Rep

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Anthracnose caused by the fungal pathogen C. sublineola is an economically important constraint on worldwide sorghum production. The most effective strategy to safeguard yield is through the introgression of resistance alleles. This requires elucidation of the genetic basis of the different resistance sources that have been identified. In this study, 223 recombinant inbred lines (RILs) derived from crossing anthracnose-differentials QL3 (96 RILs) and IS18760 (127 RILs) with the common susceptible parent PI609251 were evaluated at four field locations in the United States (Florida, Georgia, Texas, and Puerto Rico) for their anthracnose resistance response. Both RIL populations were highly susceptible to anthracnose in Florida and Georgia, while in Puerto Rico and Texas they were segregating for anthracnose resistance response. A genome scan using a composite linkage map of 982 single nucleotide polymorphisms (SNPs) detected two genomic regions of 4.31 and 0.85 Mb on chromosomes 4 and 8, respectively, that explained 10–27% of the phenotypic variation in Texas and Puerto Rico. In parallel, a subset of 43 RILs that contained 67% of the recombination events were evaluated against anthracnose pathotypes from Arkansas (2), Puerto Rico (2) and Texas (4) in the greenhouse. A genome scan showed that the 7.57 Mb region at the distal end of the short arm of chromosome 5 is associated with the resistance response against the pathotype AMP-048 from Arkansas. Comparative analysis identified the genomic region on chromosome 4 overlaps with an anthracnose resistance locus identified in another anthracnose-differential line, SC414-12E, indicating this genomic region is of interest for introgression in susceptible sorghum germplasm. Candidate gene analysis for the resistance locus on chromosome 5 identified an R-gene cluster that has high similarity to another R-gene cluster associated with anthracnose resistance on chromosome 9.

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Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes

Cited by 16 publications

References 70 publications

Cytological observation and transcriptome analysis reveal dynamic changes of Rhizoctonia solani colonization on leaf sheath and different genes recruited between the resistant and susceptible genotypes in rice

Cytological observation and transcriptome analysis reveal dynamic changes of Rhizoctonia solani colonization on leaf sheath and different genes recruited between the resistant and susceptible genotypes in rice

Physio‐biochemical and histological alterations in response to sheath blight disease of rice (Oryza sativaL.)

The inheritance of anthracnose (Colletotrichum sublineola) resistance in sorghum differential lines QL3 and IS18760

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