Comparative Transcriptomic Analysis Identifies Key Cellulose Synthase Genes (CESA) and Cellulose Synthase-like Genes (CSL) in Fast Growth Period of Flax Stem (<i>Linum Usitatissimum</i> L.)

Guo, Yuan; Wen, Lan; Chen, Jikang; Pan, Gen; Wu, Zhimin; Zheng, Li; Xiao, Qingmei; Yuan-chang, Wang; Qiu, Caisheng; Long, Songhua; Zhao, Xinlin; Wang, Hui; Wang, Yufu

doi:10.1080/15440478.2021.1993510

Cited by 4 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fourth gene ( Lus10008932 ) encodes a cytosolic or nuclear polyketide cyclase/dehydrase and lipid transport superfamily protein, also known as MLP-like protein 328 (Major Latex protein-). A previous study showed that MLP-like protein 328 transcripts were the most abundant in flax stem tissues above the snap point ( Guo et al., 2021 ), which is the transition zone in development process of fiber cells ( Gorshkova et al., 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of new potential molecular actors related to fiber quality in flax through Omics

et al. 2023

View full text Add to dashboard Cite

One of the biggest challenges for a more widespread utilization of plant fibers is to better understand the different molecular factors underlying the variability in fineness and mechanical properties of both elementary and scutched fibers. Accordingly, we analyzed genome-wide transcription profiling from bast fiber bearing tissues of seven different flax varieties (4 spring, 2 winter fiber varieties and 1 winter linseed) and identified 1041 differentially expressed genes between varieties, of which 97 were related to cell wall metabolism. KEGG analysis highlighted a number of different enriched pathways. Subsequent statistical analysis using Partial Least-Squares Discriminant Analysis showed that 73% of the total variance was explained by the first 3 X-variates corresponding to 56 differentially expressed genes. Calculation of Pearson correlations identified 5 genes showing a strong correlation between expression and morphometric data. Two-dimensional gel proteomic analysis on the two varieties showing the most discriminant and significant differences in morphometrics revealed 1490 protein spots of which 108 showed significant differential abundance. Mass spectrometry analysis successfully identified 46 proteins representing 32 non-redundant proteins. Statistical clusterization based on the expression level of genes corresponding to the 32 proteins showed clear discrimination into three separate clusters, reflecting the variety type (spring-/winter-fiber/oil). Four of the 32 proteins were also highly correlated with morphometric features. Examination of predicted functions for the 9 (5 + 4) identified genes highlighted lipid metabolism and senescence process. Calculation of Pearson correlation coefficients between expression data and retted fiber mechanical measurements (strength and maximum force) identified 3 significantly correlated genes. The genes were predicted to be connected to cell wall dynamics, either directly (Expansin-like protein), or indirectly (NAD(P)-binding Rossmann-fold superfamily protein). Taken together, our results have allowed the identification of molecular actors potentially associated with the determination of both in-planta fiber morphometrics, as well as ex-planta fiber mechanical properties, both of which are key parameters for elementary fiber and scutched fiber quality in flax.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of new potential molecular actors related to fiber quality in flax through Omics

et al. 2023

View full text Add to dashboard Cite

show abstract

The Mechanisms Underlying Physiological and Molecular Responses to Waterlogging in Flax

Qiu

Peng

et al. 2023

Journal of Natural Fibers

View full text Add to dashboard Cite

Genome-Wide Identification and Expression Analysis of CesA Gene Family in Corymbia citriodora

ChuBiao

Huang

et al. 2023

Forests

View full text Add to dashboard Cite

The CesA proteins are key players in cellulose synthesis. Eucalyptus is well-known for cellulose synthesis, although the mechanism of cellulose synthesis is largely unknown. In this study, 11 CcCesA genes were identified by comprehensive bioinformatics analysis in Corymbia citriodora, a eucalypt species. CcCesA genes were generally conserved in each subfamily. Among them, four genes lacked a transmembrane domain in the N-terminal. We showed that genes of CesA2, 4, 5, and 8 may regulate the synthesis of secondary cell walls (SCWs) through phylogenetic trees analysis. Two pairs of segmental duplication and one pair of tandem duplication were detected in CcCesAs. Analysis of Ka/Ks ratios revealed that the duplicated genes were under negative or purifying selection. Numerous cis-acting elements related to plant hormones and light reactions were identified in the promoters of CcCesAs. Expression analysis confirmed that genes of CesA2, 4, and 8 regulate the synthesis of SCWs, and CesA8, in particular, had the highest expression compared with other genes. The results will help us understand the complexity of the CcCesAs in different diameters at breast height and provide valuable information for future functional characterization of specific genes in C. citriodora.

show abstract

Comparative Transcriptomic Analysis Identifies Key Cellulose Synthase Genes (CESA) and Cellulose Synthase-like Genes (CSL) in Fast Growth Period of Flax Stem (Linum Usitatissimum L.)

Cited by 4 publications

References 32 publications

Identification of new potential molecular actors related to fiber quality in flax through Omics

Identification of new potential molecular actors related to fiber quality in flax through Omics

The Mechanisms Underlying Physiological and Molecular Responses to Waterlogging in Flax

Genome-Wide Identification and Expression Analysis of CesA Gene Family in Corymbia citriodora

Contact Info

Product

Resources

About