Comparative transcriptomic analysis of crab hemocytes in response to white spot syndrome virus or Vibrio alginolyticus infection

Zhu, Faming; Qian, Xiyi; Ma, Xiongchao

doi:10.1016/j.fsi.2018.06.003

Cited by 25 publications

(6 citation statements)

References 33 publications

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“…We have been devoted to studying the genetic breeding and aquaculture industry for many years, keeping ahead of the artificial breeding of this crab. Currently, there are no genome assembly datasets for this crab, and only several transcriptome libraries have been reported 15–19 . The only genetic linkage map for the mud crab was constructed by our team using 212 microsatellite and amplified fragment length polymorphism (AFLP) markers, and the mean spacing was 18.68 cM 20 .…”

Section: Introductionmentioning

confidence: 99%

Genome survey, high-resolution genetic linkage map construction, growth-related quantitative trait locus (QTL) identification and gene location in Scylla paramamosain

Zhao

Wang

Chen

et al. 2019

Sci Rep

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Scylla paramamosain is one of the most economically important crabs in China. In this study, the first genome survey sequencing of this crab was performed, and the results revealed that the estimated genome size was 1.21 Gb with high heterozygosity (1.3%). Then, RAD technology was used to construct a high-resolution linkage map for this species. A total of 24,444 single nucleotide polymorphism (SNP) makers were grouped into 47 linkage groups. The total length of the linkage groups was 3087.53 cM with a markers interval of 0.92 cM. With the aid of transcriptome and genome scaffold data, 4,271 markers were linked to genes, including several important growth-related genes such as transforming growth factor-beta regulator I, immune related-gene C-type lectin and ecdysone pathway gene broad-complex-like protein. Further, 442 markers, representing 279 QTLs, associated with 24 traits were identified, and of these markers, 78 were linked to genes. Some interesting genes, such as dedicator of cytokinesis protein 3, tenascin-X and DNA helicase MCM8, were believed to have important relationship with specific traits and merit further exploration. The results of this study will accelerate the genetic improvement and genome sequencing analysis of the mud crab.

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Section: Introductionmentioning

confidence: 99%

Genome survey, high-resolution genetic linkage map construction, growth-related quantitative trait locus (QTL) identification and gene location in Scylla paramamosain

Zhao

Wang

Chen

et al. 2019

Sci Rep

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“…Bacterial infection is an important biological factor restricting the development of shrimp culture. V. alginolyticus is one of the major pathogens causing mass mortality of shrimps worldwide, affecting energy metabolism, immune response and development of shrimps (52)(53)(54). Our previous studies have shown that the infection of V. alginolyticus can affect the survival, growth and metabolism of shrimp, induce the production of ROS, apoptosis and autophagy, reduce immune function, and cause DNA damage (22,(55)(56)(57).…”

Section: Discussionmentioning

confidence: 99%

Andrographolide promote the growth and immunity of Litopenaeus vannamei, and protects shrimps against Vibrio alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway

et al. 2022

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Vibrio alginolyticus (V. alginolyticus) is one of the major pathogens causing mass mortality of shrimps worldwide, affecting energy metabolism, immune response and development of shrimps. In the context of the prohibition of antibiotics, it is necessary to develop a drug that can protect shrimp from V. alginolyticus. Andrographolide (hereinafter called Andr), a traditional drug used in Chinese medicine, which possesses diverse biological effects including anti-bacteria, antioxidant, immune regulation. In this study, we investigated the effect of Andr on growth, immunity, and resistance to V. alginolyticus infection of Litopenaeus vannamei (L. vannamei) and elucidate the underlying molecular mechanisms. Four diets were formulated by adding Andr at the dosage of 0 g/kg (Control), 0.5 g/kg, 1 g/kg, and 2 g/kg in the basal diet, respectively. Each diet was randomly fed to one group with three replicates of shrimps in a 4-week feeding trial. The results showed that dietary Andr improved the growth performance and non-specific immune function of shrimps. L. vannamei fed with Andr diets showed lower mortality after being challenged by V. alginolyticus. After 6 h of V. alginolyticus infection, reactive oxygen species (ROS) production, tissue injury, apoptosis, expression of inflammatory factors (IL-1 β and TNFα) and apoptosis-related genes (Bax, caspase3 and p53) were increased in hemocytes and hepatopancreas, while feeding diet with 0.5 g/kg Andr could inhibit the increase. Considering that JNK are important mediators of apoptosis, we examined the influence of Andr on JNK activity during V. alginolyticus infection. We found that Andr inhibited JNK activation induced by V. alginolyticus infection on L. vannamei. The ROS scavenger N-acetyl-l-cysteine (NAC) suppressed V. alginolyticus-induced inflammation and apoptosis, suggesting that ROS play an important role in V. alginolyticus-induced inflammation and apoptosis. Treated cells with JNK specific activator anisomycin, the inflammation and apoptosis inhibited by Andr were counteracted. Collectively, Andr promote the growth and immunity of L. vannamei, and protects shrimps against V. alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway. These results improve the understanding of the pathogenesis of V. alginolyticus infection and provide clues to the development of effective drugs against V. alginolyticus.

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“…paramamosain infected with V . alginolyticus based on the upregulation of Relish, an NF‐κB protein regulated by the IMD pathway, which subsequently controls the expression of antimicrobial peptides 149 …”

Section: Functional Categories Of Transcriptomics In Portunid Crabsmentioning

confidence: 99%

“…Similar involvement of the IMD pathway was also reported in the haemolymph transcriptome profiles of S. paramamosain infected with V. alginolyticus based on the upregulation of Relish, an NF-κB protein regulated by the IMD pathway, which subsequently controls the expression of antimicrobial peptides. 149…”

Section: Bacteriamentioning

confidence: 99%

“…153 Based on their similar expression patterns, several genes have been implicated in the immune response of portunid crabs against both viral (WSSV) and bacterial (V. alginolyticus) infections, including glutaminyl-peptide cyclotransferase, troponin T and carboxypeptidase A. 149 Further validation studies of these genes could facilitate the discovery of a functional immune target against both viral and bacterial infections in the near future.…”

Section: Virusesmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomics in advancing portunid aquaculture: A systematic review

Waiho

Ikhwanuddin

Afiqah‐Aleng

et al. 2022

Reviews in Aquaculture

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The rapid advancement of next-generation sequencing technologies has promoted the use of transcriptomics in non-model organisms, including species in the aquaculture sector. Compared to that of other crustacean species, portunid crab aquaculture is impeded by the insufficient knowledge of their biological and physiological processes. This systematic review summarised the advances in transcriptomics in cultured portunid crabs using a systematic literature review methodology. The filtered transcriptome dataset comprised 66 articles from four genera: Scylla, Portunus, Cal-

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Comparative transcriptomic analysis of crab hemocytes in response to white spot syndrome virus or Vibrio alginolyticus infection

Cited by 25 publications

References 33 publications

Genome survey, high-resolution genetic linkage map construction, growth-related quantitative trait locus (QTL) identification and gene location in Scylla paramamosain

Genome survey, high-resolution genetic linkage map construction, growth-related quantitative trait locus (QTL) identification and gene location in Scylla paramamosain

Andrographolide promote the growth and immunity of Litopenaeus vannamei, and protects shrimps against Vibrio alginolyticus by regulating inflammation and apoptosis via a ROS-JNK dependent pathway

Transcriptomics in advancing portunid aquaculture: A systematic review

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