Comparative transcriptomic analysis reveals key genes and pathways in two different cadmium tolerance kenaf (Hibiscus cannabinus L.) cultivars

Chen, Peng; Li, Zengqiang; Luo, Dengjie; Jia, Ruixing; Lu, Hai; Tang, Meiqiong; Hu, Yijie; Jiao, Yue; Huang, Zhen

doi:10.1016/j.chemosphere.2020.128211

Cited by 68 publications

(27 citation statements)

References 58 publications

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“…However, in our experiment, in Cd 2+ -treated plants, the light energy used for photochemistry (Φ PSII ) decreased (Figure 2), and at the same time, the accumulation of MDA, indicating the degree of oxidative stress, increased (Figure 5a). Insufficient supply of energy for photochemistry decreases photosynthesis efficiency, which is detrimental to plant growth [92]. Zinc application to the nutrient solution, together with Cd 2+ , resulted in a 10-fold increase in Zn 2+ in the leaves, which increased the fraction of energy used for photochemistry, compared with Cd 2+ alone (Figure 2), while it decreased oxidative stress by 17%, also compared with Cd 2+ alone (Figure 5a).…”

Section: Discussionmentioning

confidence: 99%

Excess Zinc Supply Reduces Cadmium Uptake and Mitigates Cadmium Toxicity Effects on Chloroplast Structure, Oxidative Stress, and Photosystem II Photochemical Efficiency in Salvia sclarea Plants

Sperdouli

Adamakis

Dobrikova

et al. 2022

Toxics

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Salvia sclarea L. is a Cd2+ tolerant medicinal herb with antifungal and antimicrobial properties cultivated for its pharmacological properties. However, accumulation of high Cd2+ content in its tissues increases the adverse health effects of Cd2+ in humans. Therefore, there is a serious demand to lower human Cd2+ intake. The purpose of our study was to evaluate the mitigative role of excess Zn2+ supply to Cd2+ uptake/translocation and toxicity in clary sage. Salvia plants were treated with excess Cd2+ (100 μM CdSO4) alone, and in combination with Zn2+ (900 μM ZnSO4), in modified Hoagland nutrient solution. The results demonstrate that S. sclarea plants exposed to Cd2+ toxicity accumulated a significant amount of Cd2+ in their tissues, with higher concentrations in roots than in leaves. Cadmium exposure enhanced total Zn2+ uptake but also decreased its translocation to leaves. The accumulated Cd2+ led to a substantial decrease in photosystem II (PSII) photochemistry and disrupted the chloroplast ultrastructure, which coincided with an increased lipid peroxidation. Zinc application decreased Cd2+ uptake and translocation to leaves, while it mitigated oxidative stress, restoring chloroplast ultrastructure. Excess Zn2+ ameliorated the adverse effects of Cd2+ on PSII photochemistry, increasing the fraction of energy used for photochemistry (ΦPSII) and restoring PSII redox state and maximum PSII efficiency (Fv/Fm), while decreasing excess excitation energy at PSII (EXC). We conclude that excess Zn2+ application eliminated the adverse effects of Cd2+ toxicity, reducing Cd2+ uptake and translocation and restoring chloroplast ultrastructure and PSII photochemical efficiency. Thus, excess Zn2+ application can be used as an important method for low Cd2+-accumulating crops, limiting Cd2+ entry into the food chain.

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Section: Discussionmentioning

confidence: 99%

Excess Zinc Supply Reduces Cadmium Uptake and Mitigates Cadmium Toxicity Effects on Chloroplast Structure, Oxidative Stress, and Photosystem II Photochemical Efficiency in Salvia sclarea Plants

Sperdouli

Adamakis

Dobrikova

et al. 2022

Toxics

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“…The total RNA from the anthers was extracted according to a modified cetyltrimethylammonium bromide (CTAB) RNA extraction protocol. The RNA purity and quantification were evaluated with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States), and the RNA integrity was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States) (Chen et al, 2021). cDNA libraries were then constructed with a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, United States) according to the manufacturer's instructions.…”

Section: Transcriptome Sequencing and Data Analysismentioning

confidence: 99%

“…Kenaf (Hibiscus cannabinus L.) is a valuable multipurpose natural fiber crop species. Kenaf biomass is used for paper, cordage, building materials, automotive interiors, carpet backing, animal bedding, and livestock forage (Chen et al, 2021;Li et al, 2021). Kenaf is a thermophilic and short-day plant species that cannot or rarely flowers in northern China because of the prevailing long-day conditions; this is especially true for late-maturing varieties.…”

Section: Introductionmentioning

confidence: 99%

Integrated Methylome and Transcriptome Analyses Reveal the Molecular Mechanism by Which DNA Methylation Regulates Kenaf Flowering

Tang

Luo

et al. 2021

Front. Plant Sci.

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DNA methylation regulates key biological processes in plants. In this study, kenaf seedlings were pretreated with the DNA methylation inhibitor 5-azacytidine (5-azaC) (at concentrations of 0, 100, 200, 400, and 600 μM), and the results showed that pretreatment with 200 μM 5-azaC promoted flowering most effectively. To elucidate the underlying mechanism, phytohormone, adenosine triphosphate (ATP), and starch contents were determined, and genome-wide DNA methylation and transcriptome analyses were performed on anthers pretreated with 200 μM 5-azaC (5-azaC200) or with no 5-azaC (control conditions; 5-azaC0). Biochemical analysis revealed that 5-azaC pretreatment significantly reduced indoleacetic acid (IAA) and gibberellic acid (GA) contents and significantly increased abscisic acid (ABA) and ATP contents. The starch contents significantly increased in response to 200 and 600 μM 5-azaC. Further genome-wide DNA methylation analysis revealed 451 differentially methylated genes (DMGs) with 209 up- and 242 downregulated genes. Transcriptome analysis showed 3,986 differentially expressed genes (DEGs), with 2,171 up- and 1,815 downregulated genes. Integrated genome-wide DNA methylation and transcriptome analyses revealed 72 genes that were both differentially methylated and differentially expressed. These genes, which included ARFs, PP2C, starch synthase, FLC, PIF1, AGL80, and WRKY32, are involved mainly in plant hormone signal transduction, starch and sucrose metabolism, and flowering regulation and may be involved in early flowering. This study serves as a reference and theoretical basis for kenaf production and provides insights into the effects of DNA methylation on plant growth and development.

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“…The results show that JB231AC had some tolerance to Cd toxicity. Chen et al (2021) showed that after 10 days of Cd treatment, plant height and biomass of kenaf seedlings were significantly inhibited under 10 mg L –1 Cd stress compared to the control. Saidi et al (2021) also showed that plant growth was significantly impacted by an increasing Cd dose, and was severely suppressed at the highest Cd concentrations (50 and 100 μM) after Cd treatment for 4 days.…”

Section: Discussionmentioning

confidence: 96%

Physiological and Transcriptomic Comparison of Two Sunflower (Helianthus annuus L.) Cultivars With High/Low Cadmium Accumulation

Zhatova

et al. 2022

Front. Plant Sci.

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The toxic heavy metal cadmium (Cd) is easily absorbed and accumulated in crops and affects human health through the food chains. Sunflower (Helianthus annuus L.) is a globally important oil crop. In this study, two sunflower cultivars 62\3 (high Cd) and JB231AC (low Cd), were chosen to compare physiological and transcriptomic responses at different Cd concentrations (0, 25, 50, and 100 μM). The results showed that JB231AC had better Cd tolerance than 62\3. The contents of H2O2 and MDA (malondialdehyde) in 62\3 were lower than that in JB231AC under Cd stress, but the activities of SOD (superoxide dismutase) and POD (peroxidase) in JB231AC were higher than in 62\3, which indicated that JB231AC had a strong ability to remove reactive oxygen species (ROS)-induced toxic substances. Many deferentially expressed ABC (ATP-binding cassette) and ZIP (Zn-regulated transporter, Iron-regulated transporter-like protein) genes indicated that the two gene families might play important roles in different levels of Cd accumulation in the two cultivars. One up-regulated NRAMP (Natural resistance-associated macrophage protein) gene was identified and had a higher expression level in 62\3. These results provide valuable information to further understand the mechanism of Cd accumulation and provide insights into breeding new low Cd sunflower cultivars.

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Comparative transcriptomic analysis reveals key genes and pathways in two different cadmium tolerance kenaf (Hibiscus cannabinus L.) cultivars

Cited by 68 publications

References 58 publications

Excess Zinc Supply Reduces Cadmium Uptake and Mitigates Cadmium Toxicity Effects on Chloroplast Structure, Oxidative Stress, and Photosystem II Photochemical Efficiency in Salvia sclarea Plants

Excess Zinc Supply Reduces Cadmium Uptake and Mitigates Cadmium Toxicity Effects on Chloroplast Structure, Oxidative Stress, and Photosystem II Photochemical Efficiency in Salvia sclarea Plants

Integrated Methylome and Transcriptome Analyses Reveal the Molecular Mechanism by Which DNA Methylation Regulates Kenaf Flowering

Physiological and Transcriptomic Comparison of Two Sunflower (Helianthus annuus L.) Cultivars With High/Low Cadmium Accumulation

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