Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress

Li, Xiang; Mao, Xiuhong; Xu, Yujin; Li, Yan; Zhao, Nan; Yao, Junxiu; Dong, Yanjun; Tigabu, Mulualem; Zhao, Xiyang; Li, Shanwen

doi:10.1016/j.ecoenv.2021.112179

Cited by 30 publications

(15 citation statements)

References 107 publications

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“…The antioxidant DEGs from this study can be compared with a number of other transcriptomic studies on metal stress in trees where glutathione S ‐transferase, peroxidases, and thioredoxins were in common. For example, cadmium accumulators Salix integra and Populus × canadensis ‘Neva’ both expressed superoxide dismutase with glutathione pathway genes and peroxidases, respectively (Li, Mao, et al., 2021b ; Shi et al., 2016 ). Nickel stress in resistant versus susceptible genotypes of Betula papyrifera found glutathione S ‐transferase and thioredoxins upregulated in resistant trees (Theriault et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Strategies of tolerance reflected in two North American maple genomes

et al. 2022

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Maples (the genus Acer) represent important and beloved forest, urban, and ornamental trees distributed throughout the Northern hemisphere. They exist in a diverse array of native ranges and distributions, across spectrums of tolerance or decline, and have varying levels of susceptibility to biotic and abiotic stress. Among Acer species, several stand out in their importance to economic interest. Here we report the first two chromosome-scale genomes for North American species, Acer negundo and Acer saccharum. Both assembled genomes contain scaffolds corresponding to 13 chromosomes, with A. negundo at a length of 442 Mb, an N50 of 32 Mb, and 30 491 genes, and A. saccharum at a length of 626 Mb, an N50 of 46 Mb, and 40 074 genes. No recent whole genome duplications were detected, though A. saccharum has local gene duplication and more recent bursts of transposable elements, as well as a large-scale translocation between two chromosomes. Genomic comparison revealed that A. negundo has a smaller genome with recent gene family evolution that is predominantly contracted and expansions that are potentially related to invasive tendencies and tolerance to abiotic stress. Examination of RNA sequencing data obtained from A. saccharum given long-term aluminum and calcium soil treatments at the Hubbard Brook Experimental Forest provided insights into genes involved in the aluminum stress response at the systemic level, as well as signs of compromised processes upon calcium deficiency, a condition contributing to maple decline.

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Section: Discussionmentioning

confidence: 99%

Strategies of tolerance reflected in two North American maple genomes

et al. 2022

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“…TRXs, which play a role in cellular redox systems by facilitating the reduction of other proteins via their dithiol-disulfide active site, reduce oxidative stress and other environmental stresses, protect proteins from oxidative aggregation and inactivation to promote protein folding, regulate apoptosis via denitrosylation, and modulate inflammation [ 47 , 56 ]. Like TRXs, GRXs are a kind of thioldisulfide oxidoreductases involved in the protection and repair of protein and nonprotein thiols under oxidative stress [ 57 , 58 ]. In the present study, one TRXs encoding genes (Asia04G000080), and two GRX encoding genes (Asia10G001567 and Asia07G000485) were upregulated in leaves ( Supplementary Table S5 ), suggesting that they contribute to Cd tolerance in C. gigantea plants.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L.

Yang

Zhang

et al. 2022

IJMS

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Calotropis gigantea is often found in mining areas with heavy metal pollution. However, little is known about the physiological and molecular response mechanism of C. gigantea to Cd stress. In the present study, Cd tolerance characteristic of C. gigantea and the potential mechanisms were explored. Seed germination test results showed that C. gigantea had a certain Cd tolerance capacity. Biochemical and transcriptomic analysis indicated that the roots and leaves of C. gigantea had different responses to early Cd stress. A total of 176 and 1618 DEGs were identified in the roots and leaves of C. gigantea treated with Cd compared to the control samples, respectively. Results indicated that oxidative stress was mainly initiated in the roots of C. gigantea, whereas the leaves activated several Cd detoxification processes to cope with Cd, including the upregulation of genes involved in Cd transport (i.e., absorption, efflux, or compartmentalization), cell wall remodeling, antioxidant system, and chelation. This study provides preliminary information to understand how C. gigantea respond to Cd stress, which is useful for evaluating the potential of C. gigantea in the remediation of Cd-contaminated soils.

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“…The response to high-dose Cd exposure could be ascribed to the plant signal perception and transduction [75,76] and reduction of photosynthetic carbon assimilation [77]. The calcium ion, ABA signal conduction pathway, and mitogen-activated protein kinase (MAPK) pathway were identified as the main signal perception pathway in response to stress tolerance of plants [28,75]. In this study, the member PYR/PYL and PP2Cs of the ABA signal transduction pathway were activated under Cd stress in T. repens.…”

Section: Discussionmentioning

confidence: 99%

“…The most important enzymes include catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and glutathione reductase (GR) [25], and ascorbic acid (ASA), GSH is a non-enzyme and reduced GSH the main non-enzymatic antioxidant can be oxidized to glutathione disulfide (GSSG) to scavenge ROS [22,26]. Furthermore, the transporter family also has an important role under Cd stress, such as heavy metal ATPase (HMA), ATP-binding cassette (ABC), natural resistance-associated macrophage protein (Nramp), and cation diffusion facilitator (CDF) [27,28].…”

Section: Introductionmentioning

confidence: 99%

Unraveling Cadmium Toxicity in Trifolium repens L. Seedling: Insight into Regulatory Mechanisms Using Comparative Transcriptomics Combined with Physiological Analyses

Fan

et al. 2022

IJMS

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Trifolium repens (T. repens) can accumulate significant amounts of heavy metal ions, and has strong adaptability to wide environmental conditions, and relatively large biomass, which is considered a potential plant for phytoremediation. However, the molecular mechanisms of T. repens involved in Cd tolerance have not yet been studied in detail. This study was conducted to examine the integrative responses of T. repens exposed to a high-level CdCl2 by investigating the physiological and transcriptomic analyses. The results suggested that T. repens seedlings had a high degree of tolerance to Cd treatment. The roots accumulated higher Cd concentration than leaves and were mainly distributed in the cell wall. The content of MDA, soluble protein, the relative electrolyte leakage, and three antioxidant enzymes (POD, SOD, and APX) was increased with the Cd treatment time increasing, but the CAT enzymes contents were decreased in roots. Furthermore, the transcriptome analysis demonstrated that the differentially expressed genes (DEGs) mainly enriched in the glutathione (GSH) metabolism pathway and the phenylpropanoid biosynthesis in the roots. Overexpressed genes in the lignin biosynthesis in the roots might improve Cd accumulation in cell walls. Moreover, the DEGs were also enriched in photosynthesis in the leaves, transferase activity, oxidoreductase activity, and ABA signal transduction, which might also play roles in reducing Cd toxicity in the plants. All the above, clearly suggest that T. repens employ several different mechanisms to protect itself against Cd stress, while the cell wall biosynthesis and GSH metabolism could be considered the most important specific mechanisms for Cd retention in the roots of T. repens.

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Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress

Cited by 30 publications

References 107 publications

Strategies of tolerance reflected in two North American maple genomes

Strategies of tolerance reflected in two North American maple genomes

Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L.

Unraveling Cadmium Toxicity in Trifolium repens L. Seedling: Insight into Regulatory Mechanisms Using Comparative Transcriptomics Combined with Physiological Analyses

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