Comparative transcriptomic and physiological analyses of weedy rice and cultivated rice to identify vital differentially expressed genes and pathways regulating the ABA response

Abstract

Weedy rice is a valuable germplasm resource characterized by its high tolerance to both abiotic and biotic stresses. Abscisic acid (ABA) serves as a regulatory signal in plant cells as part of their adaptive response to stress. However, a global understanding of the response of weedy rice to ABA remains to be elucidated. In the present study, the sensitivity to ABA of weedy rice (WR04-6) was compared with that of temperate japonica Shennong9816 (SN9816) in terms of seed germination and post-germination growth via the application of exogenous ABA and diniconazole, an inhibitor of ABA catabolism. Physiological analysis and a transcriptomic comparison allowed elucidation of the molecular and physiological mechanisms associated with continuous ABA and diniconazole treatment. WR04-6 was found to display higher ABA sensitivity than SN9816, resulting in the rapid promotion of antioxidant enzyme activity. Comparative transcriptomic analyses indicated that the number of differentially expressed genes (DEGs) in WR04-6 seedlings treated with 2 μM ABA or 10 μM diniconazole was greater than that in SN9816 seedlings. Genes involved in stress defense, hormone signal transduction, and glycolytic and citrate cycle pathways were highly expressed in WR04-6 in response to ABA and diniconazole. These findings provide new insight into key processes mediating the ABA response between weedy and cultivated rice.

Molecular Responses and Degradation Mechanisms of the Herbicide Diuron in Rice Crops

“…Herein, we found that some Phase II-associated genes with high response to DU treatments in rice were reported to be regulated by phytohormones. For example, LOC_Os06g20790 ( OsMDS5 ), a carboxyl methyltransferase gene, which is highly responsive to DU stress, was shown to be regulated by auxin and determined rice inflorescence architecture . Furthermore, the KEGG pathway of DU-induced DEGs gathered the “α-linolenic acid metabolism″ pathway, which is mainly responsible for the synthesis of JA (Figure E).…”

“…For example, LO-C_Os06g20790 (OsMDS5), a carboxyl methyltransferase gene, which is highly responsive to DU stress, was shown to be regulated by auxin and determined rice inflorescence architecture. 46 Furthermore, the KEGG pathway of DUinduced DEGs gathered the "α-linolenic acid metabolism″ pathway, which is mainly responsible for the synthesis of JA (Figure 4E). Likewise, DU-responsive LOC_Os01g52260 (OsSAT1;1) encoding serine acetyltransferase could be induced by JA and ABA.…”

OsNAC15 Regulates Tolerance to Zinc Deficiency and Cadmium by Binding to OsZIP7 and OsZIP10 in Rice

“…OsNAC15 was previously reported to be mainly induced by abiotic stress treatments (cold, drought, salt, submergence, laid-down submergence and ABA) and biotic stress (rice blast) [ 63 , 64 , 65 , 66 ]. OsNAC15 was also strongly induced by As (III) treatment after 12 h and 24 h in shoots of upland rice [ 67 ].…”