Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in <i>Ananas comosus</i> var. <i>bracteatus</i>

Xue, Ying; Ma, Jun; He, Yehua; Yu, Shuang; Lin, Zhen Yuan; Xiong, Yingyuan; Rafique, Fatima; Jiang, Fuxing; Sun, Lina; Ma, Min; Zhou, Yujue; Li, Xi; Huang, Zhuo

doi:10.7717/peerj.7261

Cited by 12 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggested that the function of photosynthesis and energy metabolism might be suppressed in the Wh parts of chimeric leaves. This is accordance with our comparative proteomic data studied previously [13].…”

Section: The Proteome Profile Was Altered In the Wh Parts Of Chimericsupporting

confidence: 94%

“…Whereas upregulated proteins in the Wh parts were mainly enriched in spliceosome and ribosome through previous study [13]. Proteins with downregulated Ksu sites in the Wh parts were enriched in pathways involving the CAC, carbon metabolism, glyoxylate metabolism, dicarboxylate metabolism, pyruvate metabolism, and 1oxocarboxylic acid metabolism.…”

Section: The Succinylome Profile Was Changed In the Wh Parts Of Chimementioning

confidence: 58%

“…Proteins with downregulated Ksu sites in the Wh parts were enriched in pathways involving the CAC, carbon metabolism, glyoxylate metabolism, dicarboxylate metabolism, pyruvate metabolism, and 1oxocarboxylic acid metabolism. And previous study has shown that lots of downregulated proteins in the Wh parts were enriched in photosynthesis and respiration [13], which indicated that protein abundant and succinylation level may work together to regulate photosynthesis and respiration in chimeric leaves. These results suggested that the changed succinylation level may be a candidate regulator to metabolism-and energy-related processes of chimeric leaves in Ananas comosus var.…”

Section: The Succinylome Profile Was Changed In the Wh Parts Of Chimementioning

confidence: 91%

“…A great many of genes have been studied to analyze the mechanism of chimeric leaves formation and growth in Ananas comosus var. bracteatus [10][11][12][13]. However, the PTM-mediated regulatory mechanism in chimeric leaves of Ananas comosus var.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus

Mao

Xue

et al. 2020

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background: Lysine succinylation, an important protein posttranslational modification (PTM), is widespread and conservative. The regulatory functions of succinylation in leaf color has been reported. The chimeric leaves of Ananas comosus var. bracteatus are composed of normal green parts and albino white parts. However, the extent and function of lysine succinylation in chimeric leaves of Ananas comosus var. bracteatus has yet to be investigated. Results: Compared to the green (Gr) parts, the global succinylation level was increased in the white (Wh) parts of chimeric leaves according to the Western blot and immunohistochemistry analysis. Furthermore, we quantitated the change in the succinylation profiles between the Wh and Gr parts of chimeric leaves using label-free LFQ intensity. In total, 855 succinylated sites in 335 proteins were identified, and 593 succinylated sites in 237 proteins were quantified. Compared to the Gr parts, 232 (61.1%) sites in 128 proteins were quantified as upregulated targets, and 148 (38.9%) sites in 70 proteins were quantified as downregulated targets in the Wh parts of chimeric leaves using a 1.5-fold threshold (P < 0.05). These proteins with altered succinylation level were mainly involved in crassulacean acid metabolism (CAM) photosynthesis, photorespiration, glycolysis, the citric acid cycle (CAC) and pyruvate metabolism. Conclusions: Our results suggested that the changed succinylation level in proteins might function in the main energy metabolism pathways-photosynthesis and respiration. Succinylation might provide a significant effect in the growth of chimeric leaves and the relationship between the Wh and Gr parts of chimeric leaves. This study not only provided a basis for further characterization on the function of succinylated proteins in chimeric leaves of Ananas comosus var. bracteatus but also provided a new insight into molecular breeding for leaf color chimera.

show abstract

Section: The Proteome Profile Was Altered In the Wh Parts Of Chimericsupporting

confidence: 94%

Section: The Succinylome Profile Was Changed In the Wh Parts Of Chimementioning

confidence: 58%

Section: The Succinylome Profile Was Changed In the Wh Parts Of Chimementioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus

Mao

Xue

et al. 2020

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…During spring and autumn, the leaves will appear bright red, which improved a lot in ornamental value of Ananas comosus var. bracteatus [19]. However, in summer and winter, the redness of the leaves gradually fades, returning to green/white.…”

Section: Introductionmentioning

confidence: 99%

Light intensity affects the coloration and structure of chimeric leaves ofAnanas comosus var. bracteatus

Yang

Lin

Xue

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Ananas comosus var. bracteatus is an important tropical ornamental plant because of its green/white chimeric leaves. The accumulation of anthocyanin makes the leaf red especially the white margin. However, the leaves lost red color in summer and winter. Light intensity is one of the most important factors affecting leaf color along the season. In order to understand the effects of light intensity on the growth and coloration of the chimeric leaves, Ananas comosus var. bracteatus was grown under full sunlight, 50% shade and 75% shade for 75 days to evaluate the content of pigments, the color parameters (value L*, a*, b*) and structural histocytology characteristics of chimeric leaves. The results showed that high irradiance was beneficial to keep the chimeric leaves red. However, prolonged exposure to high irradiance caused light damage, some of the leaves wrinkled and even burned. Shading decreased the content of anthocyanin and increased the content of chlorophyll especially in the white margin of the leaves. Numerous chloroplasts were found in the mesophyll cells of the white margin part of chimeric leaves under shading for 75 days. The increase of chlorophyll content resulted in better growth of plants. In order to balance the growth and ornamental value of the leaves, approximately 50% shade is suggested to be the optimum light irradiance condition for Ananas comosus var. bracteatus in summer.

show abstract

State of the Art of Omics Technologies in Ornamental Plant Research

Naresh,

Tomar,

Singh

2024

Ornamental Horticulture: Latest Cultivation Practices and Breeding Technologies

View full text Add to dashboard Cite

Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in Ananas comosus var. bracteatus

Cited by 12 publications

References 34 publications

Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus

Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus

Light intensity affects the coloration and structure of chimeric leaves ofAnanas comosus var. bracteatus

State of the Art of Omics Technologies in Ornamental Plant Research

Contact Info

Product

Resources

About