Comparative transcriptomics and metabolomics reveal specialized metabolite drought stress responses in switchgrass (<i>Panicum virgatum</i>)

Tiedge, Kira; Li, Xingxing; Merrill, Amy T.; Davisson, Danielle; Chen, Yuxuan; Yu, Ping; Tantillo, Dean J.; Last, Robert L.; Zerbe, Philipp

doi:10.1111/nph.18443

Cited by 38 publications

(28 citation statements)

References 60 publications

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“…We purified and elucidated the structure for a novel abietane type diterpenoid -Di287 -from switchgrass roots. In contrast to the previously identified Panicoloid diterpenoids (Di301 and Di317, Tiedge et al, 2022), the NMR evidence indicated lack of a furan moiety in the diterpene scaffold of Di287 (Fig S 4 -12). We then explored SAR of three different types of switchgrass diterpenes -Di301, Di317 and Di287 -with and without a furan moiety.…”

Section: B -D and Fig S2)contrasting

confidence: 91%

“…(A) The structures of HPLC purified switchgrass saponins and diterpenoids used in the bioassays. The structures of the saponins D415-SCG, D415, D431 and D457, and the diterpenoids, Di301 and Di317, were previously elucidated by LC-MS/MS and NMR (Li et al, 2022; Tiedge et al, 2022). NMR data supporting the structure of diterpenoid Di287 can be accessed in the supplementary files ( Fig S 4 – 12, Table S7) .…”

Section: Resultsmentioning

confidence: 99%

“…These observations indicate that a furan ring increases the switchgrass diterpenoid antifungal activities, while oxidation of this moiety results in lower potency. Regardless of the structural characteristics, significantly increased accumulations of all three diterpenoid types were observed in the roots of drought stressed switchgrass (Tiedge et al, 2022), suggesting potential functions of these structurally diverse terpenoids beyond their antifungal activities.…”

Section: B -D and Fig S2)mentioning

confidence: 98%

“…n = 3 (cultivars) x 6 (replicates per cultivar) = 18. (Li et al, 2022;Tiedge et al, 2022). NMR data supporting the structure of diterpenoid Di287 can be accessed in the supplementary files (Fig S 4 -12, Table S7).…”

Section: Statistical Analysis For the Untargeted Metabolomics Analysismentioning

confidence: 99%

“…As part of a larger effort to develop switchgrass varieties with high yield and low environmental impacts, we are engaged in studies of switchgrass metabolome screening by liquid chromatography–mass spectrometry (LC–MS) and other analytical chemistry methods. We previously identified close to 100 terpenoid metabolites, including saponins, diterpenes and sesquiterpenes, in the northern upland and southern lowland switchgrass ecotypes (Li et al, 2022; Tiedge et al, 2022). We demonstrated specific differences in metabolites across the terpenoid classes driven by ecotype, tissue/developmental stage and soil moisture, raising questions about their possible biological functions.…”

Section: Introductionmentioning

confidence: 99%

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Identification of anti-fungal bioactive terpenoids from the bioenergy crop switchgrass (Panicum virgatum)

Chou

Bonito

et al. 2023

Preprint

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Plant derived bioactive small molecules have attracted attention of scientists across fundamental and applied scientific disciplines. We seek to understand the influence of these phytochemicals on functional phytobiomes. Increased knowledge of specialized metabolite bioactivities could inform strategies for sustainable crop production. Our recent investigations of metabolomes of the upland and lowland ecotypes of the bioenergy crop switchgrass revealed large differences in types and abundances of terpenoid specialized metabolites. We hypothesized that - consistent with accumulating evidence that switchgrass genotype impacts microbiome assembly - differential terpenoid accumulation contributes to switchgrass ecotype-specific microbiome composition. An initial plate-based disc diffusion screen of 18 switchgrass root derived fungal isolates revealed differential responses to upland- and lowland-isolated metabolites. To identify specific fungal growth-modulating metabolites, we tested fractions from root extracts on three ecologically important fungal isolates - Linnemania elongata, Trichoderma sp. and Fusarium sp. Saponins and diterpenoids were identified as the most prominent antifungal metabolites. Finally, analysis of liquid chromatography-purified terpenoids revealed fungal inhibition structure - activity relationships (SAR). Saponin antifungal activity was primarily determined by the number of sugar moieties - saponins glycosylated at a single core position were inhibitory whereas saponins glycosylated at two core positions were inactive. Saponin core hydroxylation and acetylation were also associated with reduced activity. Diterpenoid activity required the presence of an intact furan ring for strong fungal growth inhibition. This study demonstrates that switchgrass genotype-specific metabolites differentially inhibit fungal isolates from the root and rhizosphere, supporting the hypothesis that these molecules contribute to microbiome assembly and function.

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Section: B -D and Fig S2)contrasting

confidence: 91%