Comparative Transcriptomics ofBuzura suppressaria(Lepidoptera: Geometridae) Assembled De Novo Yield Insights Into Response AfterBuzura suppressariaNuclear Polyhedrosis Virus Infection

Abstract

Buzura suppressaria Guenee (Lepidoptera: Geometridae) is a defoliator that seriously harms eucalyptus trees in South China. Buzura suppressaria nuclear polyhedrosis virus (BsNPV) is a baculovirus that infects B. suppressaria with high specificity and efficiency. Transcriptomes of B. suppressaria were sequenced before and after BsNPV infection using an Illumina-based platform to probe for differentially expressed genes (DEGs) of B. suppressaria after viral infection. On average, ∼57.4 million high-quality clean reads were generated and assembled de novo into 69,761 unigenes. The NCBI nonredundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene ontology (GO), and Cluster of Orthologous Groups databases were used to annotate unigenes through NCBI BLAST; 33,575 unigenes (48.1%) were then mapped to at least one of these databases, and 4,366 unigenes (6.3%) were mapped to all databases. Differential expression analysis showed that 25,212 unigenes were upregulated and 22,880 unigenes were downregulated in at least one pairwise comparison. Control versus 48 h had more DEGs than other two pairwise comparisons in either the GO or KEGG database, because the number of regulated gene would increase as BsNPV infected more tissues and would decrease as more tissues were disabled. To ascertain B. suppressaria immune response to BsNPV infection, DEGs were annotated to the GO and KEGG databases. In total, 89 GO categories are related to immune response and 1,007 DEGs are annotated to these GO categories. Furthermore, 7 downregulated DEGs and 37 upregulated were obtained simultaneously in all three groups. These DEGs were considered to possess a central role throughout viral infection.

Influence of Heat Treatment on Tea Polyphenols and Their Impact on Improving Heat Tolerance in Drosophila melanogaster

“…Sample RNA was extracted by applying the TRIzol method, and the cDNA library was analyzed by using sequencing-by-synthesis technology with the Illumina HiSeq high-throughput sequencing platform [7] (Hidehiro et al, 2017). The obtained high-quality reads were then analyzed bioinformatically using a 2100 Bioanalyzer (Agilent), and functional annotation was carried out by comparing the unigenes with the GO and KEGG databases [8] (Luo et al, 2017).…”

PI3K/Akt/CncC signaling pathway mediates the response to EPN‐Bt infection in Holotrichia parallela larvae

“…22 Recently, RNA sequencing (RNA-Seq) has been widely used in the study of insect stress response, and this technique has emerged as a reliable method for identifying insect antioxidant and detoxifying enzyme genes. [23][24][25] Through transcriptome sequencing, some oxidative stress-related genes, such as SOD, POD, and metallothionein, were identified from C. medinalis after C. medinalis granulovirus infection, and these genes were involved in the metabolic process of ROS. 26 A previous transcriptome study on Drosophila melanogaster responses to Steinernema carpocapsae infection indicated that genes associated with stress response and metabolic processes were strongly induced.…”

“…Recently, RNA sequencing (RNA‐Seq) has been widely used in the study of insect stress response, and this technique has emerged as a reliable method for identifying insect antioxidant and detoxifying enzyme genes 23–25 . Through transcriptome sequencing, some oxidative stress‐related genes, such as SOD , POD , and metallothionein , were identified from C. medinalis after C .…”

Bioinformatics analysis to identify possible mechanisms of action of curcumin against tea geometrid

“…Although chemical treatment is the most effective method to control E. obliqua , growing consumer interest in tea as a healthy beverage and concerns over environmental impact of chemical treatment (Kergunteuil et al., ; Sun et al., ; Yang et al., ) have led to widespread adoption of biological control measures in China to produce safer (contaminant‐free) and organic teas (Li et al., ; Zhang et al., ). Such control measures often involve botanical insecticides, which show low toxicity in mammals and are easily degraded; thus far, a few natural products or plant extracts, such as curcumin, allicin and alkaloids, have shown promising activities against various agricultural pests (Liu et al., ; Luo et al., ).…”