Comparative transcriptomics reveals candidate transcription factors involved in costunolide biosynthesis in medicinal plant-Saussurea lappa

Thakur, Vasundhara; Bains, Savita; Pathania, Shivalika; Sharma, Shailesh; Kaur, Rajveer; Singh, Kashmir

doi:10.1016/j.ijbiomac.2020.01.312

Cited by 17 publications

(10 citation statements)

References 83 publications

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“…The transcriptional analysis suggested that EsMYBF1 and EsMYBA1, together with EsTTG1 (a WD40 TF) and EsGL3 (a bHLH TF), were probably involved in the coordinated regulation of synthesis of the flavonol-derived bioactive components and anthocyanins [81]. Thakur et al (2020) demonstrated that the MYB family of TFs was an interacting partner of SlCOS1 (costunolide synthase gene), which catalyzed the final key step of costunolide (sesquiterpene lactone) biosynthesis in Saussurea lappa [94]. In Antirrhinum majus, AmMYB305 activates the gene encoding phenylalanine ammonia-lyase (PAL) when it is co-expressed in tobacco protoplasts [95].…”

Section: The Role Of Myb Tfs In the Biosynthesis Of Flavonoids Secondmentioning

confidence: 99%

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

Cao

et al. 2020

Biology

176

103

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MYB transcription factors (TFs), as one of the largest gene families in plants, play important roles in multiple biological processes, such as plant growth and development, cell morphology and pattern building, physiological activity metabolism, primary and secondary metabolic reactions, and responses to environmental stresses. The function of MYB TFs in crops has been widely studied, but few studies have been done on medicinal plants. In this review, we summarized the MYB TFs that play important roles in secondary metabolism and emphasized the possible mechanisms underlying how MYB TFs are regulated at the protein, posttranscriptional, and transcriptional levels, as well as how they regulate the downstream target gene networks related to secondary metabolism in plants, especially in medicinal plants.

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Section: The Role Of Myb Tfs In the Biosynthesis Of Flavonoids Secondmentioning

confidence: 99%

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

Cao

et al. 2020

Biology

176

103

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“…Beyond that, hexaploid D. sinicus aggravated this difficulty, so the number of TF members obtained in this study may be higher than the actual numbers. Similarly, in the medicinal plant Saussurea lappa , 24,610 unigenes from 58 TF families were obtained by analysing comparative transcriptomics [ 39 ]. Even so, the results of this study, combined with GO and KEGG functional classifications, WGCNA co-expression networks, and protein interaction networks, can still provide valuable information for revealing the transcriptional regulation network on the growth and development of woody bamboos.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide analysis reveals core transcriptional regulators associated with culm development and variation in Dendrocalamus sinicus, the strongest woody bamboo in the world

Chen

Dou

et al. 2022

Heliyon

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“…Besides citral, the lemongrass essential oil also contains minor amounts of other monoterpenes such as geraniol, citronellal, and myrcene (Mukarram et al ., 2021). Our previous study in lemongrass and a recent study by other researchers in different Cymbopogon spp, involving transcriptomics, gene expression analyses, and molecular modelling, showed evidence for the presence of ADH and AKR encoding genes, indicating the potential involvement of any of these enzymes in citral biosynthesis (Meena et al ., 2016; Bhat et al ., 2023). Here, we have explored the biosynthesis of citral and its origin in lemongrass using a combination of biochemical, reverse and forward genetics, and molecular approaches.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetically distant enzymes localized in cytosol and plastids drive citral biosynthesis in lemongrass

Gupta,

Sharma,

Kiran

et al. 2024

Preprint

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Except for the genetic basis of citral-forming alcohol dehydrogenases (ADHs) in Litsea cubeba tree, and biochemical studies on citral-forming enzymes from select plants, knowledge regarding in-planta biosynthesis of citral and its metabolic origin remains limited. Here, we have elucidated the functions of an ADH (CfADH1) and an aldoketo-reductase (CfAKR2b) in citral biosynthesis in lemongrass (Cymbopogon flexuosus), one of the most cultivated aromatic crops for its citral-rich essential oil. Expression of both CfADH1 and CfAKR2b showed correlation with citral accumulation in different developmental stages. Recombinant CfADH1 and CfAKR2b, despite their sequence unrelatedness, exhibited similar kinetic properties and formed citral from geraniol with NADP cofactor. Virus-induced gene silencing in lemongrass, and transient expression in lemon balm (Melissa officinalis), demonstrated the in-planta involvement of CfADH1 and CfAKR2b in citral biosynthesis. While CfADH1 exhibited a dual cytosolic/plastidial localization, CfAKR2b was localized to cytosol. Moreover, feeding lemongrass seedlings with mevalonate- and methylerythritol-phosphate-pathway specific inhibitors combined with volatile profiling supported the role of both pathways in citral formation. Our results demonstrate phylogenetically distant enzymes localized in cytosol and plastids drive citral biosynthesis in lemongrass, indicating an evolutionary scenario aimed at maximizing the utilization of precursor pools from both cytosolic and plastidial pathways for high citral production.

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Comparative transcriptomics reveals candidate transcription factors involved in costunolide biosynthesis in medicinal plant-Saussurea lappa

Cited by 17 publications

References 83 publications

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

Transcriptome-wide analysis reveals core transcriptional regulators associated with culm development and variation in Dendrocalamus sinicus, the strongest woody bamboo in the world

Phylogenetically distant enzymes localized in cytosol and plastids drive citral biosynthesis in lemongrass

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