Comparative Ultrasonographic, Anatomotopographic and Macromorphometric Study of the Spleen and Pancreas in Rabbit (Oryctolagus cuniculus)

Dimitrov, R.

doi:10.15835/nsb437852

Cited by 6 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are no significant variations macroscopic structures or microarchitecture between the rabbit and rodent spleens 72 . Duplication of the spleen has been observed sporadically 73 (Rinke, pers.…”

Section: Section 6: Hematopoietic and Lymphoid Systemmentioning

confidence: 85%

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Rabbit

et al. 2021

View full text Add to dashboard Cite

The INHAND (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions Project (www.toxpath.org/inhand. asp) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP) and North America (STP) to develop an internationally accepted nomenclature for proliferative and non-proliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature for classifying microscopic lesions observed in most tissues and organs from the laboratory rabbit used in nonclinical safety studies. Some of the lesions are illustrated by color photomicrographs. The standardized nomenclature presented in this document is also available electronically on the internet (http://www.goreni.org/). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous lesions as well as lesions induced by exposure to test materials. Relevant infectious and parasitic lesions are included as well. A widely accepted and utilized international harmonization of nomenclature for lesions in laboratory animals will provide a common language among regulatory and scientific research organizations in different countries and increase and enrich international exchanges of information among toxicologists and pathologists.

show abstract

Section: Section 6: Hematopoietic and Lymphoid Systemmentioning

confidence: 85%

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Rabbit

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The morphological measurements of the spleen in hamsters differed from the authors' observations, attributing those differences to the animals’ size, relative weight, and shape of the spleen. The spleen of hamster is shorter compared to the spleen of rabbits and rats, Dimitrov (2012) and Voloshin et al . (2014) mentioned that the length of the spleen in rabbits and adult albino rats was 33.3 and 49.8 mm, respectively, while it was longer than mice and guinea pig spleen.…”

Section: Discussionmentioning

confidence: 96%

“…The width of hamsters’ spleen is narrower in contrast to other animals, the authors ( Dimitrov, 2012 ; Voloshin et al , 2014 ; Qasem et al , 2015 ; Uwah et al , 2021 ) reported that spleen widths in rats, guinea pigs, rabbits, and mice were 14, 12.2, 5.44, and 8,1 mm respectively.…”

Section: Discussionmentioning

confidence: 99%

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Hasan¹,

Altaey²,

Sultan³

2023

Open Vet J

View full text Add to dashboard Cite

Background: The golden hamster is a choice model for investigating many visceral and splenic infections and neoplastic and retrospective lesions. Aim: To study hamsters' spleen's morphological, histological, and histochemical structure. Methods: Samples were collected from eight healthy adult golden hamsters and then fixed with 10% buffered formalin. Later, samples were processed, sectioned, and stained with Hematoxylin and Eosin as well as Masson’s Trichrome stain. Other slides were further stained with Periodic Acid Schiff and Alcian blue 2.5 stain (PAS) for histochemical evolution; the gross measurement was performed for the splenic length, width, and thickness, while the histological measures included the splenic capsular and trabecula thickness, diameter of white pulp follicles, splenic sinusoids and central arteries and proportion of white and red pulps. Results: The macroscopic findings revealed that the spleen was red-brown lanciform on the left side of the dorsolateral abdominal wall. The morphological measurements for splenic length, width, and thickness were 26.6 ± 7.67, 4.17 ± 1.65, and 1.70 ± 0.01 mm, respectively. The histological observations showed that the splenic capsule was composed of two layers (serosal and subserosal). The inner layer sends trabeculae dividing the splenic parenchyma irregularly, and the splenic parenchyma comprises the white and red pulp. The white pulp follicles included the mantle, marginal zones, and the PALS (periarterial lymphatic sheath), while the red pulp constituted splenic cords and sinuses. The histomorphological findings showed that white pulp follicles and the central artery mean diameter were 252.62 ± 8.07 µm and 54.45 ± 0.36 µm respectively, the proportion of white to a red pulp was 0.49 ± 0.01, the splenic capsule, trabecula and the wall of splenic arteries showed an intense positive activity to PAS stain and negative or weak in other splenic structures. Conclusion: The similarities and differences in the spleen between the laboratory animals and hamsters were apparent in this article, so understanding the morphological and histological structure of the spleen presents significant assistance with species identification to select the appropriate experimental animal model in future medical research

show abstract

“…The rabbit spleen has been described as an elongated organ with a 3-5 cm length and a width of 0.6-1.2 cm, with the dorsal extremity adjacent to the last two ribs and cranial to the left kidney and the remainder of the spleen being located at the level of the costal arch under the abdominal wall, between the greater curvature of the stomach, the jejunum, and the caecum. 7,11,13,16,17 The spleen is attached to the greater curvature of the stomach via the gastrosplenic ligament. The phrenicosplenic ligament is only rudimentarily developed.…”

Section: Discussionmentioning

confidence: 99%

Computed tomographic features of the normal spleen in rabbits (Oryctolagus cuniculus domesticus)

Chernev,

Procter,

Isaac

et al. 2023

Vet Radiology Ultrasound

View full text Add to dashboard Cite

Computed tomography is commonly utilized for investigation of rabbits, and more recently, for evaluating abdominal pathology. The spleen, however, is an often‐overlooked organ, with limited information published. The aims of this retrospective, observational, study were to document the visibility, size, and shape of the normal rabbit spleen and potential correlations with signalment. Institutional imaging archives were reviewed for diagnostic‐image‐quality abdominal CT studies of rabbits. . In 115 cases, the inclusion criteria were met. Pre‐ and postcontrast CT studies were evaluated by two reviewers for visibility of the spleen. For precontrast CT images, the interrater agreement for identification of the spleen was fair. For postcontrast CT images, interrater agreement was moderate . There were significantly more spleens clearly identified on postcontrast studies compared with precontrast CT . Splenic location, volume, shape, X‐ray attenuation, and length were measured, and the splenic‐volume‐to‐body‐weight ratio was calculated. The mean splenic volume was 1 mL (range 0.2–3.9 mL), mean length 40 mm (range 20–61 mm), mean attenuation (precontrast CT 80 HU and postcontrast CT 320 HU), and mean splenic volume/body weight ratio was 0.5 mL/kg (range 0.17–1.2 mL/kg). There was a significant relationship between splenic volume and body weight , which was weakly positively correlated . There was no correlation between splenic volume, age, and sex. The most commonly identified splenic shapes were “banana”, “tongue”, and “elephant trunk”. The rabbit spleen can be identified on CT images, but more reliably on postcontrast CT images, which underlines the usefulness of contrast‐enhanced CT in this species.

show abstract

Comparative Ultrasonographic, Anatomotopographic and Macromorphometric Study of the Spleen and Pancreas in Rabbit (Oryctolagus cuniculus)

Cited by 6 publications

References 19 publications

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Rabbit

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Rabbit

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Computed tomographic features of the normal spleen in rabbits (Oryctolagus cuniculus domesticus)

Contact Info

Product

Resources

About