Comparative ultrasound evaluation of human trabecular bone graft properties after treatment with different sterilization procedures

Abstract

New sterilization methods for human bone are likely to affect the mechanical properties of human cancellous grafts. These mechanical properties dictate the short- and mid-term results of the orthopedic procedure. The aim of this study was to compare the effects on bone mechanical properties, as assessed by ultrasound velocity, of different sterilization methods used under similar conditions: bleach and sublimation, humid heat, successive baths of physiological saline with osmotic detersion, and CO(2) in the supercritical phase. Alterations in mechanical properties were small with CO(2) (velocity change: -2%) and humid heat (-2.5%). Osmotic detersion had a significant but moderate effect (-4.7%). The -9% change with the protocol involving bleach suggested a greater than 30% decrease in load to failure, based on earlier studies. Gamma irradiation of defatted trabecular allografts, in a dose of 10 or 25 KGy, produced no significant changes in ultrasound velocity. Powerful protein denaturants used in sterilization protocols substantially alter the mechanical resistance of the grafts, which may jeopardize the orthopedic procedure.

Long-Term Clinical and Radiographic Outcome of Extraction Socket Grafting with a Supercritical CO2Viral-Inactivated Allogeneic Bone Graft

“…Preclinical studies have shown that this process used on bone has neutral effects on the bone tissue composition; preserves its architecture and mechanical properties, particularly its high wettability, and thus improves performance [15][16][17].…”

One-Stage ACL Revision Using a Bone Allograft Plug for a Semianatomic Tibial Tunnel That Is Too Anterior

“…Our technique has several advantages ( Table 2 ): The existing tibial tunnel is filled completely with allograft bone, whose mechanical properties and architecture are identical to those of fresh bone. 23 , 24 , 25 This allows us to drill a new tibial tunnel without having to worry about overlap. Since the existing tibial tunnel is filled, the revision can be carried out like a primary ACL reconstruction procedure, with no need to worry about the previous tunnel.…”

Vreden Russian Research Institute of Traumatology and Orthopedics

“…Необходимость очистки кости от органиче-ских компонентов обусловлена двумя аспек-тами: снижением риска передачи инфекции и снижением антигенного и воспалительного потенциала [28]. по мнению R. Lomas с соавто-рами, очистка повышает клиническую эффек-тивность и безопасность материалов [3].…”