Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic<i>Escherichia coli</i>in a<i>Galleria mellonella</i>model

Ciesielczuk, Holly; Betts, Jonathan W.; Phee, Lynnette; Doumith, Michel; Hope, Russell; Woodford, Neil; Wareham, David W.

doi:10.4161/21505594.2014.988095

Cited by 53 publications

(47 citation statements)

References 38 publications

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“…MICs were determined specifically for this study using the BSAC methodology published previously (BSAC, 2011;Ciesielczuk et al, 2015) and EUCAST breakpoints (www.eucast.org/clinical_breakpoints; version 2.0, 2012). Both urinary (32 mg ml 21 ) and systemic (8 mg ml 21 ) breakpoints were used for …”

Section: Methodsmentioning

confidence: 99%

Characterization of the extra-intestinal pathogenic Escherichia coli ST131 clone among isolates recovered from urinary and bloodstream infections in the United Kingdom

Ciesielczuk

Doumith

Hope

et al. 2015

Journal of Medical Microbiology

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The multidrug-resistant ST131-O25b clone of Escherichia coli is well established as a significant cause of extra-intestinal infections worldwide. However, there have been only two small regional studies comparing ST131 isolates from the UK. Therefore, we characterized 143 ST131 E. coli (38 urinary, 105 bloodstream) collected between January 2011 and March 2012 from 38 centres located across the UK and Republic of Ireland. Phenotypic and genotypic characterization of clonal isolates revealed high rates of resistance to amoxicillin-clavulanate (56 %), cefotaxime (32 %), ciprofloxacin (79 %), temocillin (69 %, bloodstream isolates only), gentamicin (67 %) and trimethoprim-sulfamethoxazole (59 %). The most frequently detected extended-spectrum beta-lactamase was CTX-M-15 (87 %), predominantly encoded on IncF plasmids, although it was also associated with IncU plasmids in two isolates. The majority of UK ST131 clonal isolates possessed the O25b antigen (97 %) and the H30 fimH allele (92 %), but three serogroups (O19a, O136 and O153) novel to ST131 were identified among our strains. Contrary to previous reports, UK ST131-O16 isolates were typically susceptible to ciprofloxacin and lacked beta-lactamase genes (n512/12). In summary, ST131 strains of E. coli circulating in the UK possess characteristic clonal features, but are becoming more diverse than other international ST131 populations.

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Section: Methodsmentioning

confidence: 99%

Characterization of the extra-intestinal pathogenic Escherichia coli ST131 clone among isolates recovered from urinary and bloodstream infections in the United Kingdom

Ciesielczuk

Doumith

Hope

et al. 2015

Journal of Medical Microbiology

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“…Such conservation of function means that information gleaned by analysis of UPEC-associated fitness and virulence factors within one model system may be relevant in other environments. Consequently, the use of surrogate hosts like C. elegans and zebrafish, or even larvae of the wax moth Galleria mellonella (250)(251)(252), is not so tangential to UTI research and has the potential to drive important discoveries in human patients. Findings made by using animal model systems can be complemented and extended in broth culture or on agar plates using genetic and biochemical assays that are designed to examine how UPEC mutants and wild-type strains handle specific stresses such as reactive oxygen species or serum components like complement (197,243).…”

Section: Discussionmentioning

confidence: 99%

Strengths and Limitations of Model Systems for the Study of Urinary Tract Infections and Related Pathologies

Barber

Norton

Wiles

et al. 2016

Microbiol Mol Biol Rev

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SUMMARYUrinary tract infections (UTIs) are some of the most common bacterial infections worldwide and are a source of substantial morbidity among otherwise healthy women. UTIs can be caused by a variety of microbes, but the predominant etiologic agent of these infections is uropathogenicEscherichia coli(UPEC). An especially troubling feature of UPEC-associated UTIs is their high rate of recurrence. This problem is compounded by the drastic increase in the global incidence of antibiotic-resistant UPEC strains over the past 15 years. The need for more-effective treatments for UTIs is driving research aimed at bettering our understanding of the virulence mechanisms and host-pathogen interactions that occur during the course of these infections. Surrogate models of human infection, including cell culture systems and the use of murine, porcine, avian, teleost (zebrafish), and nematode hosts, are being employed to define host and bacterial factors that modulate the pathogenesis of UTIs. These model systems are revealing how UPEC strains can avoid or overcome host defenses and acquire scarce nutrients while also providing insight into the virulence mechanisms used by UPEC within compromised individuals, such as catheterized patients. Here, we summarize our current understanding of UTI pathogenesis while also giving an overview of the model systems used to study the initiation, persistence, and recurrence of UTIs and life-threatening sequelae like urosepsis. Although we focus on UPEC, the experimental systems described here can also provide valuable insight into the disease processes associated with other bacterial pathogens both within the urinary tract and elsewhere within the host.

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“…Bu böceği laboratuvar ortamında yetiştirirken gerek besinlerdeki mikrobiyal kontaminasyonu önlemek gerekse biyolojik, fizyolojik, biyokimyasal ve ekolojik araştırmalar için mikroorganizmadan arındırılmış böcekler yetiştirmek amacıyla bazı antibakteriyel antibiyotikler kullanılmıştır [13][14][15] . Diğer taraftan, G. mellonella larvaları yeni antimikrobiyal ilaçların etkinliği ile hastalık etkeni mikroorganizmaların (bakteriyel ve fungal) patojenitelerini belirlemede memeli model konaklara alternatif model olarak kullanılmaktadır [16,17] .…”

Section: Introductionunclassified

The Effect of Gemifloxacin on Some Biological Traits of Galleria mellonella (Lepidoptera: Pyralidae) Adults

Hiz¹,

Erdem²,

Büyükgüzel³

et al. 2016

Kafkas Univ Vet Fak Derg

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ÖzetBüyük bal mumu güvesi Galleria mellonella L. mikrobiyal enfeksiyonların ve klinik ilaçların çalışılmasında memeli modellerine alternatif bir model olarak bilindiği gibi insektisit etkinliği çalışmalarında da model böcek olarak yaygın kullanıma sahiptir. Bu böceğin larvaları yapay besin ortamında beslenerek fluorokinolon sınıfından bir antibiyotik olan gemifloksasinin böceğin eşey oranı, dişi ve erkek ömür uzunluğu, yumurta verimi, açılma oranı gibi ergin biyolojik özellikleri üzerine etkisi laboratuvar şartlarında incelendi. Böceğin birinci evre larvaları %0.001, 0.01, 0.1 ve 1.0 oranında gemifloksasin içeren yapay besinler ile ergin evreye kadar beslendi. Gemifloksasinin denenen konsantrasyonlarını içeren besinler erkek ve dişi eşey oranı ile erginlerin ömür uzunluğu üzerinde etkili olmadığı halde yumurta verimini önemli derecede düşürdüğü belirlendi. Artan besinsel gemifloksasin konsantrasyonları ile ters orantılı olarak yumurta verimi ve açılma oranı azaldı. Kontrol besini ile yetiştirilen dişiler günde 134.46 adet yumurta üretirken gemifloksasinin yüksek miktarlarında dişiler 26.75 ve 53.5 yumurta bıraktılar. Yumurtaların açılma oranı da gemifloksasinin %0.01'lik konsantrasyonundan itibaren önemli derecede azalmış olup, en yüksek konsantrasyon bu oranı %53.71'e düşürdü. Bu çalışma erginlerin biyolojik özelliklerinin gemifloksasin tarafından önemli derecede etkilendiğini ve bu etkilerin antibiyotiğin konsantrasyonlarına bağımlı değişimler olduğunu gösterdi. Anahtar sözcükler: Galleria mellonella, Gemifloksasin, Yumurta verimi, Ömür uzunluğuThe Effect of Gemifloxacin on Some Biological Traits of Galleria mellonella (Lepidoptera: Pyralidae) Adults AbstractGreater wax moth, Galleria mellonella L. is most commonly used as model insects for studying insecticidal efficiency of chemicals as well as it has been known as an alternative model to mamalian model for studying microbial infections and clinical drugs. The effects of a fluoroquinolone antibacterial agent, gemifloxacin, on sex ratio, male and female adult longevity, fecundity and hatchability of this insect were investigated by rearing the first instar larvae on artificial diets in the laboratory condition. The insect was reared from first-instar larvae to adult stage on an artificial diets containing gemifloxacin at 0.001, 0.01, 0.1 or 1.0%. Gemifloxacin did not affect male and female sex ratio and adult longevity while it significantly decreased egg number. Fecundity and hatchability were significantly and inversely decreased by increasing gemifloxacin concentrations. The females reared from control diet produced 134.46 per day, whereas high concentrations of gemifloxacin decreased the egg number to 26.75 and 53.5. Hatchability was also significantly decreased by 0.01% and above concentrations of gemifloxacin whereas, the highest concentration of antibiotic lowered the hatchability to 53.71%. This study indicated sublethal effects of gemifloxacin are likely to have a significant impact on adult biological traits and these effects show concentration-depe...

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Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenicEscherichia coliin aGalleria mellonellamodel

Cited by 53 publications

References 38 publications

Characterization of the extra-intestinal pathogenic Escherichia coli ST131 clone among isolates recovered from urinary and bloodstream infections in the United Kingdom

Characterization of the extra-intestinal pathogenic Escherichia coli ST131 clone among isolates recovered from urinary and bloodstream infections in the United Kingdom

Strengths and Limitations of Model Systems for the Study of Urinary Tract Infections and Related Pathologies

The Effect of Gemifloxacin on Some Biological Traits of Galleria mellonella (Lepidoptera: Pyralidae) Adults

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