Comparative virulence phenotypes and molecular genotypes of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen in China and the United States

Zhan, Gangming; Chen, Xianming; Kang, Zhensheng; Huang, Lili; Wang, Meinan; Wan, Anmin; Peng, Cheng; Cao, Shiyun; She-lin, Jin

doi:10.1016/j.funbio.2012.03.004

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…For various reasons, there have been only a few virulence studies to compare P. striiformis f. sp. tritici populations from different countries (30,40). To better characterize the P. striiformis f. sp.…”

Section: Discussionmentioning

confidence: 99%

Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010

2014

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A b s tr a c t Wan, A. M., and Chen, X. M. 2014. Virulence characterization of Puccinia striiformis f. sp. tritici using a new set of Yr single-gene line differentials in the United States in 2010. Plant Dis. 98:1534-1542.Puccinia striiformis f. sp. tritici causes stripe rust (yellow rust) of wheat and is highly variable in virulence toward wheat with racespecific resistance. During 2010, wheat stripe rust was the most widespread in the recorded history of the United States, resulting in large-scale application of fungicides and substantial yield loss. A new differential set with 18 yellow rust (Yr) single-gene lines was established and used to differentiate races of P. striiformis f. sp. tritici, which were named as race PSTv in distinction from the PST races identified in the past. An octal system was used to describe the virulence and avirulence patterns of the PSTv races. From 348 viable P. striiformis f. sp. tritici isolates recovered from a total of 381 wheat and grass stripe rust samples collected in 24 states, 41 races, named PSTv-1 to PSTv-41, were identified using the new set of 18 Yr single gene differentials, and their equivalent PST race names were determined on the previous set of 20 wheat cultivar differentials. The frequencies and distributions of the races and their virulences were determined. The five most predominant races were PSTv-37 (34.5%), PSTv-11 (17.5%), PSTv-14 (7.2%), PSTv-36 (5.2%), and PSTv-34 (4.9%). PSTv-37 was distributed throughout the country while PSTv-11 and PSTv-14 were almost restricted to states west of the Rocky Mountains. The races had virulence to 0 to 13 of the 18 Yr genes. Frequencies of virulences toward resistance genes Yr6, and YrExp2 were high (67.0 to 93.7%); those to Yrl (32.8%) and YrTye (31.3%) were moderate; and those to YrlO, Yr24, Yr32, and YrSP were low (3.4 to 5.7%). All of the isolates were avirulent to Yr5 and Yrl 5.

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Section: Discussionmentioning

confidence: 99%

Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010

2014

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“…tritici races as it provides very clear low and high responses with low probability of misclassification (Sharma-Poudyal et al 2013;Wan and Chen 2014;Wan et al 2016;Zhan et al 2012). Race groups separated by virulence and avirulence on Tyee correspond to genetic groups separated by molecular markers A.…”

Section: Discussionmentioning

confidence: 97%

“…tritici population in the United States can be separated into two major groups based on virulence or avirulence to YrTye, and that the two major virulence groups correspond to two major genetic groups determined using molecular markers Wan and Chen 2014;Wan et al 2016). In general, the frequencies of virulence to Tyee were low in other countries compared with the United States (Sharma-Poudyal et al 2013;Zhan et al 2012;A. M. Wan and X. M. Chen, unpublished data).…”

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Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

et al. 2016

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Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising 13 SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker wmc11 and proximally by wmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45 to 1.00. Because YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed high polymorphisms. The specificity of Yr76 is useful in differentiating P. striiformis f. sp. tritici races, and its tightly linked markers will be useful in developing resistant cultivars when combining the gene with other genes for resistance to stripe rust.

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“…Several research groups have developed molecular markers for population genetic studies of Pst (Chen et al, 1993;Shan et al, 1998;Justesen et al, 2002;Roose-Amsaleg et al, 2002;Enjalbert et al, 2005). In order to study Pst populations at the molecular level, simple sequence repeat (SSR) markers, as one group of robust molecular markers that are multi-allelic, co-dominant, abundant, and highly informative, were developed from complementary DNA (cDNA) and expressed sequence tag (EST) libraries (Enjalbert et al, 2002;Bahri et al, 2009;Chen et al, 2009;Cheng et al, 2012) and were used in population genetic studies (Zhan et al, 2012;Ali et al, 2014b) or in studies of origination and prediction of migratory trajectories (Ali et al, 2014a). However, these markers were limited in number and even uninformative (e.g.…”

Section: Introductionmentioning

confidence: 99%

Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

Zhan

Wang

Luo

et al. 2015

J. Zhejiang Univ. Sci. B

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Puccinia striiformis f. sp. tritici (Pst) is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatellite markers from the genomic sequence of an isolate of Chinese Pst race CY32. Polymorphism at each simple sequence repeat (SSR) locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 (mean 0.62) and 0.23 to 0.73 (mean 0.51), respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag (EST)-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.

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Comparative virulence phenotypes and molecular genotypes of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen in China and the United States

Cited by 26 publications

References 34 publications

Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010

Virulence Characterization of Puccinia striiformis f. sp. tritici Using a New Set of Yr Single-Gene Line Differentials in the United States in 2010

Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

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