Comparative zone electrophoresis of catalase of Staphylococcus species isolated from mammalian skin

Abstract

Vertical polyacrylamide slab gel electrophoresis was conducted for the catalase enzymes of representative strains of 18 proposed species and subspecies of the genus Staphylococcus. The catalase bands which resulted were predominantly monomorphic within each of the species and differences in catalase mobilities were observed between many of the species. The electrophoretic mobilities of the catalases were supportive to the scheme of classification used. Many strains of certain species demonstrated multiple catalase bands which are suggestive of multimolecular forms of the enzyme. Horizontal starch gel electrophoresis of representative strains of S. capitis produced catalase bands with relative mobilities that were different from those obtained with polyacrylamide electrophoresis, presumably due to a difference in molecular sieving between the gels.

Plasmid-encoded catalase of Staphylococcus simulans biovar staphylolyticus

“…1, lanes 1, 2, and 3). This catalase-banding pattern agrees with that observed by Zimmerman [4] for strains of S. simulans, including the type strain.…”

“…In each, the catalases are the products of two different genes that are regulated separately [2,3]. Many staphylococci also have been shown by electrophoretic analysis to have multiple catalase activities [4]. However, the basis for these multiple activities has not been reported.…”

A procedure for urease and protein extraction from staphylococci

“…1980). Enzymatic lysis by lysostaphin is possible but expensive, and such laborious and hazardous techniques as the French Press (Zimmerman 1976;Zimmerman & Kloos 1976) are methods of last resort. It is now evident that in addition to Staph.…”