Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer

Souza, Ana Luiza Ribeiro de; Marra, Kayla; Gunn, Jason R.; Samkoe, Kimberley S.; Kanick, Stephen C.; Davis, Scott C.; Chapman, Michael; Maytin, Edward V.; Hasan, Tayyaba; Pogue, Brian W.

doi:10.1038/bjc.2016.267

Cited by 43 publications

(38 citation statements)

References 45 publications

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“…Coutier et al reported for mTHPC that illumination with a fluence rate of 30 and 5 mW·cm −2 did not change the intratumor pO 2 and resulted in a significant longer tumor growth delay compared to higher fluence rate illuminations [32]. The rate and extent of photobleaching can be used for implicit dosimetry [3,[10][11][12]. In the present study we observed a slower rate of photobleaching for high fluence rate illuminations of 50 and 150 mW·cm −2 compared illumination with 20 mW·cm −2 .…”

Section: Discussionsupporting

confidence: 49%

“…Significantly less cell survival is observed using a fluence rate of 20 mW·cm −2 compared to 150 mW·c m −2 in-vitro and significant less tumors were cured after illumination with 150 mW·cm −2 or 50 mW·cm −2 compared to 20 mW·cm −2 in-vivo. It is well known that fluence rate is an important factor for therapeutic outcome of non-targeted PDT [3,4,10,12]. Illumination with a high fluence rate has shown to result in less tissue damage during 5-aminolevulinic acid based protoporphyrin IX PDT [3].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, a full understanding of light delivery, tissue optical properties, oxygenation levels and photosensitizer localization are important if PDT is to be optimized. We and others have shown that rate of fluorescence photobleaching of some photosensitizers during PDT is related to formation of singlet oxygen and can be used for implicit dosimetry [3,[10][11][12]. Most fluorescence measurements techniques however do not account for the influence of tissue optical properties on fluorescence signals that have been shown to be different for different tissues and change during PDT.…”

Section: Introductionmentioning

confidence: 99%

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In-Vivo Optical Monitoring of the Efficacy of Epidermal Growth Factor Receptor Targeted Photodynamic Therapy: The Effect of Fluence Rate

Peng

Bruijn

Hagen

et al. 2020

Cancers

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Targeted photodynamic therapy (PDT) has the potential to improve the therapeutic effect of PDT due to significantly better tumor responses and less normal tissue damage. Here we investigated if the efficacy of epidermal growth factor receptor (EGFR) targeted PDT using cetuximab-IRDye700DX is fluence rate dependent. Cell survival after treatment with different fluence rates was investigated in three cell lines. Singlet oxygen formation was investigated using the singlet oxygen quencher sodium azide and singlet oxygen sensor green (SOSG). The long-term response (to 90 days) of solid OSC-19-luc2-cGFP tumors in mice was determined after illumination with 20, 50, or 150 mW·cm−2. Reflectance and fluorescence spectroscopy were used to monitor therapy. Singlet oxygen was formed during illumination as shown by the increase in SOSG fluorescence and the decreased response in the presence of sodium azide. Significantly more cell death and more cures were observed after reducing the fluence rate from 150 mW·cm−2 to 20 mW·cm−2 both in-vitro and in-vivo. Photobleaching of IRDye700DX increased with lower fluence rates and correlated with efficacy. The response in EGFR targeted PDT is strongly dependent on fluence rate used. The effectiveness of targeted PDT is, like PDT, dependent on the generation of singlet oxygen and thus the availability of intracellular oxygen.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In-Vivo Optical Monitoring of the Efficacy of Epidermal Growth Factor Receptor Targeted Photodynamic Therapy: The Effect of Fluence Rate

Peng

Bruijn

Hagen

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…It has been reported that fractional (that is,intermittent) treatment with light might be as uperior approach to obtain more potent PDT effects as ac onsequence of reduced short term O 2 consumption during the PDT process. [67][68][69][70] To further enhance fractional PDT,T uran et al recently developed an interesting boron-dipyrromethene (BODIPY)based PS. [71] In this system, BODIPY is incorporated with 2pyrdidone into as ingle bifunctional compound (PYR6).…”

Section: Fractional Pdtmentioning

confidence: 99%

Innovative Strategies for Hypoxic‐Tumor Photodynamic Therapy

et al. 2018

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Despite its clinical promise, photodynamic therapy (PDT) suffers from a key drawback associated with its oxygen-dependent nature, which limits its effective use against hypoxic tumors. Moreover, both PDT-mediated oxygen consumption and microvascular damage further increase tumor hypoxia and, thus, impede therapeutic outcomes. In recent years, numerous investigations have focused on strategies for overcoming this drawback of PDT. These efforts, which are summarized in this review, have produced many innovative methods to avoid the limits of PDT associated with hypoxia.

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“…Our results show that DFO enhances PpIX fluorescence in cell monolayers, but it does not when the same cells of adherent monolayer nature are forced into suspension. Different mechanisms, such as uptake rates, enzymatic activities or cell-to-cell interaction, have been identified as playing a role in the 5-ALA-induced PpIX photoactivation following exogenous DFO administration [24,35,36]. Yet, our attempt at enhancing the metabolic activities and maintaining membrane integrity did not increase the PPIX fluorescence of suspended cells any further.…”

Section: Discussionmentioning

confidence: 78%

Probing Hexaminolevulinate Mediated PpIX Fluorescence in Cancer Cell Suspensions in the Presence of Chemical Adjuvants

Chan

Gleadle

Vasilev

et al. 2020

IJMS

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Exogenous administration of hexaminolevulinate (HAL) induces fluorescent protoporphyrin IX (PpIX) accumulation preferentially in cancer cells. However, the PpIX fluorescence intensities between noncancer and cancer cells are highly variable. The contrast between cancer and noncancer cells may be insufficient to reliably discriminate, especially at the single cell level in cancer diagnostics. This study examines the use of the chemical adjuvants dimethylsulphoxide (DMSO) or deferoxamine (DFO) to enhance the HAL induced PpIX accumulation in cancer cells. Our results showed that in some of the incubation conditions tested, the addition of DFO with HAL significantly increased PpIX 21 fluorescence of adherent monolayer cancer cells, but this was never the case for cells in suspension. Permeabilisation with DMSO did not increase PpIX fluorescence. Cell-to-cell interaction may well play an important role in the PpIX accumulation when suspended cells are treated in HAL and adjuvant chemicals.presence of intracellular PpIX. 5-ALA based PDD is used in the detection of a wide range of cancers, such as brain [5], ovarian [6], colorectal [7], breast [8] and bladder [9]. In urology, blue light cystoscopy PPD is now recommended by several guidelines around the world. Yet the underlying mechanisms responsible for the specificity of PpIX accumulation in tumour cells remains poorly understood [10]. To date, it has been attributed to the distinctive nature of cancer cells, primarily, to reduced FECH activity [11] and to the limited availability of iron in cancer cells [12,13] or to the reduced availability of nicotinamide adenine dinucleotide phosphate (NADPH) provision. However, the difference in PpIX fluorescence intensity between normal and cancer cells is highly variable from one cell type to another [14]. In fact, the contrast is, in some cases, insufficient to reliably discriminate between the two, especially when applying PDD principles ex vivo, at the single cell level [15,16]. To enable the development of noninvasive diagnostic methods using PDD on body fluid sediments, such as urine [17][18][19][20], practical ways to enhance the PpIX fluorescence contrast ex vivo are required [21]. Strategies to do so may well be different from those that are successful in vivo, simply because the cell microenvironments are drastically different in those two scenarios.

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Comparing desferrioxamine and light fractionation enhancement of ALA-PpIX photodynamic therapy in skin cancer

Cited by 43 publications

References 45 publications

In-Vivo Optical Monitoring of the Efficacy of Epidermal Growth Factor Receptor Targeted Photodynamic Therapy: The Effect of Fluence Rate

In-Vivo Optical Monitoring of the Efficacy of Epidermal Growth Factor Receptor Targeted Photodynamic Therapy: The Effect of Fluence Rate

Innovative Strategies for Hypoxic‐Tumor Photodynamic Therapy

Probing Hexaminolevulinate Mediated PpIX Fluorescence in Cancer Cell Suspensions in the Presence of Chemical Adjuvants

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