Comparing Levels of Metabolic Predictors of Coronary Heart Disease between Healthy Lean and Overweight Females

Abstract

Screening for the metabolomic signature of coronary heart disease (CHD) before disease onset could help in early diagnosis and potentially disease prevention. In this study, the levels of 17 CHD metabolic biomarkers in apparently healthy overweight females were compared to lean counterparts, and their associations with conventional clinical risk factors were determined. Clinical and metabolic data from 200 apparently healthy non-obese Qatari females were collected from Qatar Biobank (discovery cohort). Logistic regression was used to assess the association between body mass index (BMI) groups and 17 CHD metabolic biomarkers, and receiver operating characteristic (ROC) analysis was used to evaluate the prognostic value of CHD metabolic biomarkers in overweight. Stepwise linear regression was performed to identify the classical risk factors associated with CHD metabolites differentiating the two BMI groups. Validation of the association of CHD metabolic biomarkers with BMI groups was performed in 107 subjects (replication cohort). Out of the tested CHD metabolic biomarkers, five were significantly different between lean and overweight females in the discovery cohort (AUC = 0.73). Among these, the association of mannose, asparagine, and linoleate with BMI groups was confirmed in the replication cohort (AUC = 0.97). Significant correlations between predictors of CHD in overweight healthy women and classical risk factors were observed, including serum levels of cholesterol, testosterone, triiodothyronine, thyroxine, creatinine, albumin, bilirubin, glucose, c-peptide, uric acid, calcium and chloride. Apparently, healthy overweight females exhibit significantly different levels of specific CHD metabolites compared to their lean counterparts, offering a prognostic potential with preventative value.

Metabolomic profiling reveals key metabolites associated with hypertension progression

“…Alpha-linolenic acid is an essential omega-3 fatty acid highly present in edible and flaxseed oils ( 29 ), while gamma-linolenic acid is an omega-6 fatty acid, abundant in green leafy vegetables and nuts ( 45 ). Similar to our findings, a study comparing levels of metabolic predictors of coronary heart disease indicated that linolenate (18:3n3 or 6) was significantly different between lean and overweight females and was associated with elevated c-peptide and diastolic blood pressure ( 46 ). Zhe Wang et al also reported that linoleante is associated with increased risk of coronary heart disease ( 47 ).…”

Correlation Between Plasma Proteomics and Adverse Outcomes Among Older Men With Chronic Coronary Syndrome

“…On the other hand, several studies are designed to provide the genomic or metabolomic background of CHD ( 35 – 37 ). All these studies, once combined together, might provide essential evidence for the efficient risk-stratification of patients with CHD.…”